ID   CAT_CLOBU               Reviewed;         219 AA.
AC   Q02736;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 2.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Chloramphenicol acetyltransferase;
DE            Short=CAT;
DE            EC=2.3.1.28;
GN   Name=catB;
OS   Clostridium butyricum.
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1492;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1489203; DOI=10.1128/aac.36.11.2548;
RA   Huggins A.S., Bannam T.L., Rood J.I.;
RT   "Comparative sequence analysis of the catB gene from Clostridium
RT   butyricum.";
RL   Antimicrob. Agents Chemother. 36:2548-2551(1992).
CC   -!- FUNCTION: This enzyme is an effector of chloramphenicol resistance in
CC       bacteria.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + chloramphenicol = chloramphenicol 3-acetate +
CC         CoA; Xref=Rhea:RHEA:18421, ChEBI:CHEBI:16730, ChEBI:CHEBI:17698,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.28;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10021};
CC   -!- SUBUNIT: Homotrimer.
CC   -!- SIMILARITY: Belongs to the chloramphenicol acetyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; M93113; AAA73865.1; -; Genomic_DNA.
DR   PIR; A48907; A48907.
DR   RefSeq; WP_063843219.1; NG_047589.1.
DR   AlphaFoldDB; Q02736; -.
DR   SMR; Q02736; -.
DR   KEGG; ag:AAA73865; -.
DR   GO; GO:0008811; F:chloramphenicol O-acetyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.559.10; -; 1.
DR   InterPro; IPR023213; CAT-like_dom_sf.
DR   InterPro; IPR018372; Chloramphenicol_AcTrfase_AS.
DR   InterPro; IPR001707; Cmp_AcTrfase.
DR   PANTHER; PTHR38474; PTHR38474; 1.
DR   Pfam; PF00302; CAT; 1.
DR   PIRSF; PIRSF000440; CAT; 1.
DR   SMART; SM01059; CAT; 1.
DR   PROSITE; PS00100; CAT; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Antibiotic resistance; Transferase.
FT   CHAIN           1..219
FT                   /note="Chloramphenicol acetyltransferase"
FT                   /id="PRO_0000165859"
FT   ACT_SITE        190
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10021"
SQ   SEQUENCE   219 AA;  26144 MW;  1D2BB096D2C4C183 CRC64;
     MNFNLIDINH WSRKPYFEHY LNNVKCTYSM TANIEITDLL YEIKLKNIKF YPTLIYMIAT
     VVNNHKEFRI CFDHKGSLGY WDSMNPSYTI FHKENETFSS IWTEYNKSFL RFYSDYLDDI
     KNYGNIMKFT PKSNEPDNTF SVSSIPWVSF TGFNLNVYNE GTYLIPIFTA GKYFKQENKI
     FIPISIQVHH AICDGYHASR FINEMQELAF SFQEWLENK