ID   YC06_KLEPN              Reviewed;         722 AA.
AC   Q48452;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Putative tyrosine-protein kinase in cps region;
DE            EC=2.7.10.-;
DE   AltName: Full=ORF6;
OS   Klebsiella pneumoniae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Chedid;
RX   PubMed=7896702; DOI=10.1128/jb.177.7.1788-1796.1995;
RA   Arakawa Y., Wacharotayankun R., Nagatsuka T., Ito H., Kato N., Ohta M.;
RT   "Genomic organization of the Klebsiella pneumoniae cps region responsible
RT   for serotype K2 capsular polysaccharide synthesis in the virulent strain
RT   Chedid.";
RL   J. Bacteriol. 177:1788-1796(1995).
RN   [2]
RP   PHOSPHORYLATION.
RX   PubMed=11742763; DOI=10.1016/s1096-4959(01)00490-0;
RA   Preneta R., Jarraud S., Vincent C., Doublet P., Duclos B., Etienne J.,
RA   Cozzone A.J.;
RT   "Isolation and characterization of a protein-tyrosine kinase and a
RT   phosphotyrosine-protein phosphatase from Klebsiella pneumoniae.";
RL   Comp. Biochem. Physiol. 131B:103-112(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-
CC         [protein]; Xref=Rhea:RHEA:10596, Rhea:RHEA-COMP:10136, Rhea:RHEA-
CC         COMP:10137, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:46858,
CC         ChEBI:CHEBI:82620, ChEBI:CHEBI:456216;
CC   -!- PATHWAY: Glycan metabolism; exopolysaccharide biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- PTM: Autophosphorylated on tyrosine residue(s).
CC       {ECO:0000269|PubMed:11742763}.
CC   -!- SIMILARITY: Belongs to the etk/wzc family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; D21242; BAA04777.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q48452; -.
DR   SMR; Q48452; -.
DR   UniPathway; UPA00631; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004713; F:protein tyrosine kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0045226; P:extracellular polysaccharide biosynthetic process; IEA:InterPro.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR025669; AAA_dom.
DR   InterPro; IPR005702; EPS_synthesis.
DR   InterPro; IPR032807; GNVR.
DR   InterPro; IPR003856; LPS_length_determ_N_term.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF13614; AAA_31; 1.
DR   Pfam; PF13807; GNVR; 1.
DR   Pfam; PF02706; Wzz; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01007; eps_fam; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Cell inner membrane; Cell membrane;
KW   Exopolysaccharide synthesis; Kinase; Membrane; Nucleotide-binding;
KW   Phosphoprotein; Transferase; Transmembrane; Transmembrane helix;
KW   Tyrosine-protein kinase.
FT   CHAIN           1..722
FT                   /note="Putative tyrosine-protein kinase in cps region"
FT                   /id="PRO_0000212360"
FT   TRANSMEM        31..53
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        427..449
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   722 AA;  80400 MW;  3CAD6910AE81C3D7 CRC64;
     MTSISKKKQP ETVDDLDFGR MVGELIDHRK IIIALTSFAT LIALLYAFFA TPIYKADALI
     QVEQKQANAI LSNLSQMLPD SQPQSAPEIA LIQSRMILGK TVDDLNLQAV VSPKYFPIFG
     RGWARLSGEH QGNIQLSRLY VSSSIGDEEN PPEFTLKVKD SNRYVIEFGG EEINGKVGEL
     IEKDGITLKI DEINAKPGAE FTIKYVSKLK AIADLQENLS VADQGKDTGI LILSYLGDDP
     LKIKNIVDSI SENYLAQNIS RQAAQDEKSL EFLNKQLPMV RSDLDSAEDK LNDFRKRNDS
     VDLSLEAKSV LDQIVNVDNQ LNELTFRESE ISQLYTKEHP TYKALMEKRK TLQDERGKLN
     KRVATMPETQ QEILRLSRDV ESGRAVYMQL LNRQQELNIA KSSAIGNVRI IDSAVTQHKP
     VKPKKIIVVL AGLFIGLVIS VSLVLVRILL RKGIETPEQL EELGINVYAS IPVSESNPKN
     VIAKRLNKRD DSRPKVLLAT ENPADLAIEA IRGLRTSLHF AMLEARNNLL MISGASPNAG
     KTFVSSNLSS VISQTGKKVI FIDADLRKGY THKLFNIKNT NGLSDYLSGR VALDKIINNL
     QTEGFDYISR GSVPPNPAEL LMHNRLAELL EWANKSYDIV ILDTPPILAV ADAAIIGNYV
     GTTLLVARFE ENTPKEIDIS VKRFQNSGVN IKGCILNGVV KKATNKYGYG YNYYDYSYSD
     KK