YC22_ARATH
ID YC22_ARATH Reviewed; 484 AA.
AC O81916; Q940T7;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Uncharacterized calcium-binding protein At1g02270;
GN OrderedLocusNames=At1g02270; ORFNames=T6A9_9, T7I23.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC24386.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; U89959; AAC24386.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE27408.1; -; Genomic_DNA.
DR EMBL; AY052704; AAK96608.1; -; mRNA.
DR EMBL; AY063716; AAL36066.1; -; mRNA.
DR RefSeq; NP_563646.1; NM_100107.5.
DR AlphaFoldDB; O81916; -.
DR SMR; O81916; -.
DR STRING; 3702.AT1G02270.1; -.
DR PaxDb; O81916; -.
DR PRIDE; O81916; -.
DR ProteomicsDB; 228569; -.
DR EnsemblPlants; AT1G02270.1; AT1G02270.1; AT1G02270.
DR GeneID; 839381; -.
DR Gramene; AT1G02270.1; AT1G02270.1; AT1G02270.
DR KEGG; ath:AT1G02270; -.
DR Araport; AT1G02270; -.
DR TAIR; locus:2204863; AT1G02270.
DR eggNOG; KOG2338; Eukaryota.
DR HOGENOM; CLU_052248_0_0_1; -.
DR InParanoid; O81916; -.
DR OMA; VERDPSC; -.
DR OrthoDB; 468346at2759; -.
DR PhylomeDB; O81916; -.
DR PRO; PR:O81916; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O81916; baseline and differential.
DR Genevisible; O81916; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0000175; F:3'-5'-exoribonuclease activity; IBA:GO_Central.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR Gene3D; 3.60.10.10; -; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR036691; Endo/exonu/phosph_ase_sf.
DR InterPro; IPR005135; Endo/exonuclease/phosphatase.
DR Pfam; PF03372; Exo_endo_phos; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR SUPFAM; SSF56219; SSF56219; 1.
DR PROSITE; PS50222; EF_HAND_2; 1.
PE 2: Evidence at transcript level;
KW Calcium; Membrane; Metal-binding; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..484
FT /note="Uncharacterized calcium-binding protein At1g02270"
FT /id="PRO_0000073884"
FT TRANSMEM 25..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 384..419
FT /note="EF-hand"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 397
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000305"
FT BINDING 399
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000305"
FT BINDING 401
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000305"
FT BINDING 408
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000305"
SQ SEQUENCE 484 AA; 55474 MW; 9E99EB00A4129FF8 CRC64;
MQSHQNRSRL SLILRSRFIP SFSSPLSLFV VLAAVPLPIY FSGLLSGRNN KPLSVMRLNS
NLASSMVESN ISCTTFNILA PIYKRVDQKN HSTRESDFRT LWLARNQRIL DLLLHQRSSV
ICLQEVWVGN EELVNMYHHQ LSSSGYTIYQ LARTNSRGDG LLTAIHKDHF KVVNYRELLF
NDFGDRVAQL LHVKTVIPFP LNGKQDVQQE VIIVNTHLLF PHDSSLSIVR LHQVYKILEY
LEAYQKENKL NHMPIILCGD WNGSKRGHVY KFLRSQGFIS SYDDAHQYTD SDAHRWVSHR
NHRGNICGVD FIWLCNPSDS RKPLRTSWVE AVFSIIKYQL HKASIAEDDA FTFLGAKNHS
DSLTYSDFCL ALQKVNLTGI PHGLSFEETK ELWVRADLDG NGVFDYEELK KIWNMTMVNQ
PGNCKESVME SKKEEGEDEA IGLKVNKAIL FPQEAEKGLW PENYNISDHA CLTVQFSPVK
MLCS
