ID   CAT_STRAC               Reviewed;         220 AA.
AC   P20074;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Chloramphenicol acetyltransferase;
DE            Short=CAT;
DE            EC=2.3.1.28;
GN   Name=cat;
OS   Streptomyces acrimycini.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1884;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2697637; DOI=10.1016/0378-1119(89)90420-4;
RA   Murray I.A., Gil J.A., Hopwood D.A., Shaw W.V.;
RT   "Nucleotide sequence of the chloramphenicol acetyltransferase gene of
RT   Streptomyces acrimycini.";
RL   Gene 85:283-291(1989).
CC   -!- FUNCTION: This enzyme is an effector of chloramphenicol resistance in
CC       bacteria.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + chloramphenicol = chloramphenicol 3-acetate +
CC         CoA; Xref=Rhea:RHEA:18421, ChEBI:CHEBI:16730, ChEBI:CHEBI:17698,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.28;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10021};
CC   -!- SUBUNIT: Homotrimer.
CC   -!- SIMILARITY: Belongs to the chloramphenicol acetyltransferase family.
CC       {ECO:0000305}.
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DR   PIR; JQ0375; JQ0375.
DR   AlphaFoldDB; P20074; -.
DR   SMR; P20074; -.
DR   GO; GO:0008811; F:chloramphenicol O-acetyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.559.10; -; 1.
DR   InterPro; IPR023213; CAT-like_dom_sf.
DR   InterPro; IPR018372; Chloramphenicol_AcTrfase_AS.
DR   InterPro; IPR001707; Cmp_AcTrfase.
DR   PANTHER; PTHR38474; PTHR38474; 1.
DR   Pfam; PF00302; CAT; 1.
DR   PIRSF; PIRSF000440; CAT; 1.
DR   SMART; SM01059; CAT; 1.
DR   PROSITE; PS00100; CAT; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Antibiotic resistance; Transferase.
FT   CHAIN           1..220
FT                   /note="Chloramphenicol acetyltransferase"
FT                   /id="PRO_0000165878"
FT   ACT_SITE        195
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10021"
SQ   SEQUENCE   220 AA;  24936 MW;  E99F8030DC30226B CRC64;
     MDAPIPTPAP IDLDTWPRRQ HFDHYRRRVP CTYAMTVEVD VTAFAAALRR SPRKSYLAQV
     WALATVVNRH EEFRMCLNSS GDPAVWPVVH PAFTVFNPER ETFACLWAPY DPDFGTFHDT
     AAPLLAEHSR ATDFFPQGNP PPNAFDVSSL PWVSFTGFTL DIRDGWDHLA PIFTLGRYTE
     RDTRLLLPLS VQIHHAAADG FHTARLTNEL QTLLADPAWL