YCAM_ECOLI
ID YCAM_ECOLI Reviewed; 476 AA.
AC P75835; Q9R7Q7;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 25-MAY-2022, entry version 145.
DE RecName: Full=Inner membrane transporter YcaM;
GN Name=ycaM; OrderedLocusNames=b0899, JW5119;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC superfamily. {ECO:0000305}.
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DR EMBL; U00096; AAC73985.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA35634.2; -; Genomic_DNA.
DR PIR; B64829; B64829.
DR RefSeq; NP_415419.2; NC_000913.3.
DR RefSeq; WP_000918506.1; NZ_SSZK01000002.1.
DR AlphaFoldDB; P75835; -.
DR SMR; P75835; -.
DR BioGRID; 4261113; 109.
DR DIP; DIP-11468N; -.
DR STRING; 511145.b0899; -.
DR TCDB; 2.A.3.7.2; the amino acid-polyamine-organocation (apc) family.
DR PaxDb; P75835; -.
DR PRIDE; P75835; -.
DR EnsemblBacteria; AAC73985; AAC73985; b0899.
DR EnsemblBacteria; BAA35634; BAA35634; BAA35634.
DR GeneID; 945518; -.
DR KEGG; ecj:JW5119; -.
DR KEGG; eco:b0899; -.
DR PATRIC; fig|1411691.4.peg.1377; -.
DR EchoBASE; EB3461; -.
DR eggNOG; COG0531; Bacteria.
DR HOGENOM; CLU_020854_0_1_6; -.
DR InParanoid; P75835; -.
DR OMA; IVMPMRY; -.
DR PhylomeDB; P75835; -.
DR BioCyc; EcoCyc:YCAM; -.
DR PRO; PR:P75835; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006865; P:amino acid transport; IBA:GO_Central.
DR InterPro; IPR002293; AA/rel_permease1.
DR Pfam; PF13520; AA_permease_2; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..476
FT /note="Inner membrane transporter YcaM"
FT /id="PRO_0000213044"
FT TOPO_DOM 1..9
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..38
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 60..80
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 102..125
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 147..154
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 176..195
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 217..240
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 262..291
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 292..312
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 313..343
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 344..364
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 365..375
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 376..396
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 397..414
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 415..435
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 436..448
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 449..469
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 470..476
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 476 AA; 52496 MW; EEE28BE119576B21 CRC64;
MAGNVQEKQL RWYNIALMSF ITVWGFGNVV NNYANQGLVV VFSWVFIFAL YFTPYALIVG
QLGSTFKDGK GGVSTWIKHT MGPGLAYLAA WTYWVVHIPY LAQKPQAILI ALGWAMKGDG
SLIKEYSVVA LQGLTLVLFI FFMWVASRGM KSLKIVGSVA GIAMFVMSLL YVAMAVTAPA
ITEVHIATTN ITWETFIPHI DFTYITTISM LVFAVGGAEK ISPYVNQTRN PGKEFPKGML
CLAVMVAVCA ILGSLAMGMM FDSRNIPDDL MTNGQYYAFQ KLGEYYNMGN TLMVIYAIAN
TLGQVAALVF SIDAPLKVLL GDADSKYIPA SLCRTNASGT PVNGYFLTLV LVAILIMLPT
LGIGDMNNLY KWLLNLNSVV MPLRYLWVFV AFIAVVRLAQ KYKPEYVFIR NKPLAMTVGI
WCFAFTAFAC LTGIFPKMEA FTAEWTFQLA LNVATPFVLV GLGLIFPLLA RKANSK
