YCDA_BACSU
ID YCDA_BACSU Reviewed; 354 AA.
AC O34538;
DT 10-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Uncharacterized lipoprotein YcdA;
DE Flags: Precursor;
GN Name=ycdA; OrderedLocusNames=BSU02780;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9274031; DOI=10.1099/00221287-143-8-2775;
RA Kumano M., Tamakoshi A., Yamane K.;
RT "A 32 kb nucleotide sequence from the region of the lincomycin-resistance
RT gene (22 degrees-25 degrees) of the Bacillus subtilis chromosome and
RT identification of the site of the lin-2 mutation.";
RL Microbiology 143:2775-2782(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=22882210; DOI=10.1111/j.1365-2958.2012.08205.x;
RA Yepes A., Schneider J., Mielich B., Koch G., Garcia-Betancur J.C.,
RA Ramamurthi K.S., Vlamakis H., Lopez D.;
RT "The biofilm formation defect of a Bacillus subtilis flotillin-defective
RT mutant involves the protease FtsH.";
RL Mol. Microbiol. 86:457-471(2012).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC ProRule:PRU00303, ECO:0000269|PubMed:22882210}; Lipid-anchor
CC {ECO:0000255|PROSITE-ProRule:PRU00303}. Membrane raft
CC {ECO:0000269|PubMed:22882210}. Note=Present in detergent-resistant
CC membrane (DRM) fractions that may be equivalent to eukaryotic membrane
CC rafts; these rafts include proteins involved in signaling, molecule
CC trafficking and protein secretion. {ECO:0000269|PubMed:22882210}.
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DR EMBL; AB000617; BAA22239.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12072.1; -; Genomic_DNA.
DR PIR; C69755; C69755.
DR RefSeq; NP_388160.1; NC_000964.3.
DR RefSeq; WP_003234750.1; NZ_JNCM01000030.1.
DR PDB; 4R4G; X-ray; 2.62 A; A=30-354.
DR PDBsum; 4R4G; -.
DR AlphaFoldDB; O34538; -.
DR SMR; O34538; -.
DR STRING; 224308.BSU02780; -.
DR PaxDb; O34538; -.
DR EnsemblBacteria; CAB12072; CAB12072; BSU_02780.
DR GeneID; 938380; -.
DR KEGG; bsu:BSU02780; -.
DR PATRIC; fig|224308.179.peg.288; -.
DR eggNOG; ENOG5032ESZ; Bacteria.
DR OMA; DDAYGAN; -.
DR BioCyc; BSUB:BSU02780-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0045121; C:membrane raft; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR Gene3D; 2.60.40.1240; -; 1.
DR InterPro; IPR029051; DUF4352.
DR InterPro; IPR031343; DUF5105.
DR InterPro; IPR029050; Immunoprotect_excell_Ig-like.
DR Pfam; PF11611; DUF4352; 1.
DR Pfam; PF17118; DUF5105; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Lipoprotein; Membrane; Palmitate;
KW Reference proteome; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 22..354
FT /note="Uncharacterized lipoprotein YcdA"
FT /id="PRO_0000013691"
FT LIPID 22
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 22
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT STRAND 35..39
FT /evidence="ECO:0007829|PDB:4R4G"
FT STRAND 44..53
FT /evidence="ECO:0007829|PDB:4R4G"
FT STRAND 67..76
FT /evidence="ECO:0007829|PDB:4R4G"
FT STRAND 79..81
FT /evidence="ECO:0007829|PDB:4R4G"
FT STRAND 83..85
FT /evidence="ECO:0007829|PDB:4R4G"
FT HELIX 87..89
FT /evidence="ECO:0007829|PDB:4R4G"
FT STRAND 90..94
FT /evidence="ECO:0007829|PDB:4R4G"
FT STRAND 97..99
FT /evidence="ECO:0007829|PDB:4R4G"
FT HELIX 107..109
FT /evidence="ECO:0007829|PDB:4R4G"
FT STRAND 114..116
FT /evidence="ECO:0007829|PDB:4R4G"
FT STRAND 121..130
FT /evidence="ECO:0007829|PDB:4R4G"
FT STRAND 135..140
FT /evidence="ECO:0007829|PDB:4R4G"
FT HELIX 148..151
FT /evidence="ECO:0007829|PDB:4R4G"
FT STRAND 153..158
FT /evidence="ECO:0007829|PDB:4R4G"
FT HELIX 162..169
FT /evidence="ECO:0007829|PDB:4R4G"
FT HELIX 170..172
FT /evidence="ECO:0007829|PDB:4R4G"
FT HELIX 173..185
FT /evidence="ECO:0007829|PDB:4R4G"
FT HELIX 193..198
FT /evidence="ECO:0007829|PDB:4R4G"
FT HELIX 202..220
FT /evidence="ECO:0007829|PDB:4R4G"
FT HELIX 225..229
FT /evidence="ECO:0007829|PDB:4R4G"
FT HELIX 231..247
FT /evidence="ECO:0007829|PDB:4R4G"
FT STRAND 249..258
FT /evidence="ECO:0007829|PDB:4R4G"
FT STRAND 261..269
FT /evidence="ECO:0007829|PDB:4R4G"
FT HELIX 276..288
FT /evidence="ECO:0007829|PDB:4R4G"
FT HELIX 296..311
FT /evidence="ECO:0007829|PDB:4R4G"
FT STRAND 321..329
FT /evidence="ECO:0007829|PDB:4R4G"
FT HELIX 342..349
FT /evidence="ECO:0007829|PDB:4R4G"
SQ SEQUENCE 354 AA; 39180 MW; 90A2B4F2C6F7531A CRC64;
MFQKKTYAVF LILLLMMFTA ACSGSKTSAE KKESETEKSS DIAQVKIKDV SYTLPSKYDK
STSDDQLVLK VNVAVKNTGK DPLNVDSMDF TLYQGDTKMS DTDPEDYSEK LQGSTINADK
SVEGNLFFVV DKGKQYELNY TPESYGDKKP KSVTFKIDGK DKKILATADK LQDSAKALSA
YVDVLLFGKD NADFEKITGA NKNEIVNDFN ESAKDGYLSA SGLSSTYADS KALDNIVNGI
KEGLSKNSSI QAKTTSISKD EAIVEATVKP VDASSLSDRI EDKVKDYYSK NSSASYEEAV
KYALQVYPEE FKKLGPASSE KTVEVKMKKN DIDQWQLDMD DYRAAELVEA FIKE
