ID   YCF2_SPIOL              Reviewed;        2131 AA.
AC   P08973; Q9M3I2; Q9THF6; Q9THF7;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   29-AUG-2001, sequence version 2.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Protein Ycf2;
GN   Name=ycf2-A;
GN   and
GN   Name=ycf2-B;
OS   Spinacia oleracea (Spinach).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX   NCBI_TaxID=3562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2841033; DOI=10.1007/bf00365665;
RA   Zhou D.X., Massenet O., Quigley F., Marion M.J., Moneger F., Huber P.,
RA   Mache R.;
RT   "Characterization of a large inversion in the spinach chloroplast genome
RT   relative to Marchantia: a possible transposon-mediated origin.";
RL   Curr. Genet. 13:433-439(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Geant d'hiver, and cv. Monatol;
RX   PubMed=11292076; DOI=10.1023/a:1006478403810;
RA   Schmitz-Linneweber C., Maier R.M., Alcaraz J.-P., Cottet A., Herrmann R.G.,
RA   Mache R.;
RT   "The plastid chromosome of spinach (Spinacia oleracea): complete nucleotide
RT   sequence and gene organization.";
RL   Plant Mol. Biol. 45:307-315(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 191-273 AND 532-672.
RA   Downie S.R., Katz-Downie D.S., Cho K.-J.;
RT   "Relationships in the Caryophyllales as suggested by phylogenetic analyses
RT   of partial chloroplast DNA ORF2280 homolog sequences.";
RL   Am. J. Bot. 84:253-273(1997).
RN   [4]
RP   DETECTION OF THE PROTEIN.
RC   TISSUE=Green leaf;
RX   PubMed=8425053; DOI=10.1007/bf00039621;
RA   Glick R.E., Sears B.B.;
RT   "Large unidentified open reading frame in plastid DNA (ORF2280) is
RT   expressed in chloroplasts.";
RL   Plant Mol. Biol. 21:99-108(1993).
CC   -!- FUNCTION: Probable ATPase of unknown function. Its presence in a non-
CC       photosynthetic plant (Epifagus virginiana) and experiments in tobacco
CC       indicate that it has an essential function which is probably not
CC       related to photosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma.
CC   -!- MISCELLANEOUS: The detected polypeptide is 170 kDa; it is not clear
CC       which parts of the protein are stably expressed.
CC   -!- SIMILARITY: Belongs to the Ycf2 family. {ECO:0000305}.
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DR   EMBL; X07908; CAA30743.1; -; Genomic_DNA.
DR   EMBL; AJ400848; CAB88802.1; -; Genomic_DNA.
DR   EMBL; AJ400848; CAB88769.1; -; Genomic_DNA.
DR   EMBL; U48521; AAB50540.1; -; Genomic_DNA.
DR   EMBL; U48557; AAB50519.1; -; Genomic_DNA.
DR   PIR; S01446; S01446.
DR   RefSeq; NP_055004.1; NC_002202.1.
DR   AlphaFoldDB; P08973; -.
DR   STRING; 3562.P08973; -.
DR   PRIDE; P08973; -.
DR   GeneID; 2715689; -.
DR   KEGG; soe:2715689; -.
DR   OrthoDB; 10010at2759; -.
DR   Proteomes; UP000054095; Chloroplast.
DR   GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01330; Ycf2; 1.
DR   InterPro; IPR003959; ATPase_AAA_core.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR008543; Uncharacterised_Ycf2.
DR   PANTHER; PTHR33078:SF22; PTHR33078:SF22; 2.
DR   Pfam; PF00004; AAA; 1.
DR   Pfam; PF05695; DUF825; 3.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chloroplast; Nucleotide-binding; Plastid; Reference proteome.
FT   CHAIN           1..2131
FT                   /note="Protein Ycf2"
FT                   /id="PRO_0000223070"
FT   BINDING         1484..1491
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        853
FT                   /note="I -> T (in Ref. 1; CAA30743)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        937
FT                   /note="K -> N (in Ref. 1; CAA30743)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        973
FT                   /note="T -> P (in Ref. 1; CAA30743)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1946
FT                   /note="S -> T (in Ref. 1; CAA30743)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   2131 AA;  249983 MW;  915CCF87D1C32E31 CRC64;
     MKGHQFKSWI FELREILREI KNSHYFLDSW TQFNSVGSFI HIFFYQERFL KLFDPRIWSI
     LLSPNSQGST SNRYFTIKGV VLFVVVVLIY RITNRNMVER KNLYLIGLFP IPMNSIGPRN
     DTLEKSFGSS NINRLIVSLL YLPKGKKISE SYFLDPKEST WFLPITKKCI MPESNRGSRW
     WRNWIGKRRD SSCKISNETV AGIEISFKEK DIQYLEFPFV YYMDDPIRKD HDWELFDCLS
     LFLRNVSREN WIWLDNVRLV NKDRFFSKVR NVSSNIQYDF TRSSFVQVTD SSQLKESSDQ
     SRDRSNSISN ADSEYHTLIN KREIQQLKER SILRDPSFLQ TEGTEIESDR FPKCLSGYSS
     MPRLFTAREK QMIIHLLPEE IEQLLENPTR SIRSFFSGRW SELHLGSNPT ERSTRDPQLL
     KKQQDVSFAP SRQSENKEMV NIFKIIKYLQ NTVSIHPISS DPGCDMVPKD ELDMDSSDKI
     SFLNKNSFFD LFHLFHDRNR GGYALHHDFE SEEKFQEMAD LFTLSITDPD LVYHRGFSFS
     IDSCGLDQKQ FLNEVFNSRD ESKKKSLLVL SPIFYEENES FYRRIRKKGV RISRNVLNRF
     FLINRSDRSF EYGIQRDQIG NDTLNHRTIR KYMINQDFSN LKKSQKKWFD PLIFLSRTER
     FMNRDPDAYR YKWFNGSKNF QEHLEHFVSE QKSRFQVVFD QLRINQYSID WSEVIDKKDL
     SKSLRFFLSK SLRFFLSKLL LFLSNSLPFF FVSFGNIPIN RSEIRIYELK GPNDQLYNPL
     VESIGLQIVH LKKWKAFLLD DHDTFQKSKF LINGGTISPF LFNKIPKWMI DSFHTRNNSG
     KSFDNTDSYF SMISHDQNNW LNPVKPFHRS SLISSFYKAN QLRFLNNPHH FCFYCNKRFP
     FYMEKARINN SDFTYRQFLN ILFIHNKLFS LCVGKKKHAF LERDTISPIE SQVSNIFLPN
     DFPIRSDLLV RRTIYSIADI SGTPLTEGQL VHFERTYCQP LSDMNLSDSE KKNLHQYLNF
     NSNMGFIYTP CSEKYLLSEK RKKRSLCLKK CVEKGQMYRT FQRDSAFSTL SKWNLFQTYM
     PWFLTSTGYK YLNFLFLDTF SDLLPILSSS QKFLSILHDI MHGSGISWRI LQKKLCLPPW
     NLISEISSKC LHNLLLPEEM IHRNNESPLI WTHLASPNVR EFFYSILFLL FVAGYLVRTH
     LLFVFRASSE LQTEFERVKS LMIPSYMIEL RKLLDRYPTS EPNSFWLKNL FLVALEQLGD
     SLEEIRGSAS GDNMLLGGGP GPAYGFKSIR SKKKYLNINL IDILDLISII PNPINRITFS
     RNTRHLSHTS KEIYSLIRKR KRVNGDWIDD KIESWVASSD SIDDEEREFL VQFSTLTTEK
     RIDQILLSLT HSDHLSKNDS GYQLIEQPGA IYLRYLVDIH KKYLMNYEFN TSCLAERRVF
     LAHYQTITYS QTSCGANSFH FPSHGKPFSL RLALSPSRGI LVIGSIGTGR SYLVKYLATN
     SYVPFITVFL NKFLDNKPKG SLIDASDDID RDLDTELELL TMMNALTMDM MPEIDQFSIT
     LQFELAKAMS PCIIWIPNIH DLDVNESNYL SLGLFVNYLS RDCERGSTRN ILVIASTHIP
     QKVDPALIAP NQLNTCIKIR RLRIPQQRKH FFTLSYTRGF HLEKKMFHTN GFGSITMGSN
     VRDLVAFINE ALSISITQKK SIIDTNTIRS ALHRQTWDLR SQVRSVQDHG ILFYQIGRAV
     AQNVLLSNCP IDPISTYMKK KSCNEGDSYL YKWYFELGTS MKKLTILLYL LSCSAGSVAQ
     DLWSLPGPDE KNGITSYGLV ENDSYLVHGL LEVEGALVGS SRIEKACSQN DRVTLFLRPE
     LRNPLDMMQN GSCSILDHRF LYEKYESELE EGEGALDPQQ IEEDLFNHIV WAPRIWNPWG
     FLFDCIERPN ELGFPYWARS FRGKRSIYDK EDELQENDSE FLQSGTMQYQ TRDRSSKEQG
     FFRISQFIWD PADPLFFLFK DQPFVSVFSH REFFADEEIS KGLLTSQMNP PISIFQRWFI
     KNTQEKHFEL LINRQRWLRT NSSLSNGSFR SNTLSESYQY LSNLFLSNGT LLDQMTKTLL
     RKRWLFPDEM KIGFMQEEKD FPFLSRKDMW P