ID   YCF78_CHLRE             Reviewed;        1995 AA.
AC   P36495; B7U1J9; Q37303;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 2.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Uncharacterized membrane protein ycf78;
DE   AltName: Full=ORF-S;
DE   AltName: Full=ORF1995;
DE   AltName: Full=ORFA;
GN   Name=ycf78;
OS   Chlamydomonas reinhardtii (Chlamydomonas smithii).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=3055;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9065699; DOI=10.1007/s004380050368;
RA   Boudreau E., Turmel M., Goldschmidt-Clermont M., Rochaix J.-D., Sivan S.,
RA   Michaels A., Leu S.;
RT   "A large open reading frame (orf1995) in the chloroplast DNA of
RT   Chlamydomonas reinhardtii encodes an essential protein.";
RL   Mol. Gen. Genet. 253:649-653(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CC-503;
RX   PubMed=19473533; DOI=10.1186/1471-2148-9-120;
RA   Smith D.R., Lee R.W.;
RT   "Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome:
RT   addressing the mutational-hazard hypothesis.";
RL   BMC Evol. Biol. 9:120-120(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1573-1995.
RC   STRAIN=cw15;
RA   Sivan S., Michaels A.;
RL   Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1897-1995.
RX   PubMed=2876928; DOI=10.1016/0378-1119(86)90038-7;
RA   Woessner J.P., Gillham N.W., Boynton J.E.;
RT   "The sequence of the chloroplast atpB gene and its flanking regions in
RT   Chlamydomonas reinhardtii.";
RL   Gene 44:17-28(1986).
RN   [5]
RP   PARTIAL NUCLEOTIDE SEQUENCE.
RX   PubMed=8155879; DOI=10.1007/bf00023556;
RA   Boudreau E., Otis C., Turmel M.;
RT   "Conserved gene clusters in the highly rearranged chloroplast genomes of
RT   Chlamydomonas moewusii and Chlamydomonas reinhardtii.";
RL   Plant Mol. Biol. 24:585-602(1994).
RN   [6]
RP   IDENTIFICATION, AND COMPLETE PLASTID GENOME.
RX   PubMed=12417694; DOI=10.1105/tpc.006155;
RA   Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H.,
RA   Stern D.B.;
RT   "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a
RT   sea of repeats.";
RL   Plant Cell 14:2659-2679(2002).
CC   -!- FUNCTION: Essential for cell growth. May be involved in binding
CC       chloroplast DNA to either the chloroplast envelope or the thylakoid
CC       membrane.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC       Multi-pass membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the ycf78 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA84144.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X92726; CAA63385.1; -; Genomic_DNA.
DR   EMBL; M13704; AAA84144.1; ALT_INIT; Genomic_DNA.
DR   EMBL; FJ423446; ACJ50146.1; -; Genomic_DNA.
DR   EMBL; X76934; CAA54257.1; -; Genomic_DNA.
DR   EMBL; BK000554; DAA00959.1; -; Genomic_DNA.
DR   PIR; S41289; S41289.
DR   PIR; T08166; T08166.
DR   RefSeq; NP_958415.1; NC_005353.1.
DR   AlphaFoldDB; P36495; -.
DR   STRING; 3055.DAA00959; -.
DR   PaxDb; P36495; -.
DR   PRIDE; P36495; -.
DR   GeneID; 2717035; -.
DR   KEGG; cre:ChreCp059; -.
DR   HOGENOM; CLU_234019_0_0_1; -.
DR   InParanoid; P36495; -.
DR   Proteomes; UP000006906; Chloroplast.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Chloroplast; Membrane; Plastid; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..1995
FT                   /note="Uncharacterized membrane protein ycf78"
FT                   /id="PRO_0000217502"
FT   TRANSMEM        31..51
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        53..73
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        106..126
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        157..177
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        212..232
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        254..274
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        307..327
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1418..1441
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1848..1883
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1422..1441
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1848..1868
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         580
FT                   /note="L -> V (in strain: CC-503)"
FT   VARIANT         1588
FT                   /note="K -> R (in strain: CC-503 and cw15)"
FT   VARIANT         1610
FT                   /note="P -> A (in strain: CC-503)"
FT   VARIANT         1618
FT                   /note="P -> A (in strain: CC-503)"
SQ   SEQUENCE   1995 AA;  232195 MW;  E5A3F167EAF5FC8A CRC64;
     MITFTFMSLV TSVKDYVEIT HKLIEIEPLK NYTEFGAVFT YFIFSIGEFF KNFFSFSFLN
     NIWSIPIIIP DIASAMISEV SVLDGYFHNA FTFLETSVNT TTNPSLVIFE KFVIGIINSL
     FLILPTSTSH LITLRRFVMQ GLEAGYMAGL GTLAGNFLWL ASIILGWRFF VIPWLSLDIF
     RYLLGFVLLV KYIWDSSKER RMALEDLSKW KIFLLNFLLA LTEQSCIYPF ISNLSFGPDA
     SILEGFPVDN YPQFLLIHGA YLLGILFGSF SLLQFTCWFW ENPAFSIYLW ITTKSSLKIS
     TSSYYKILNF TFLYATMLCA IASIPYYGLD YTITNPIGLV PQDRILNQKK SQSDPDKLIT
     ETAFLNLNPT DKNSRIRDGV HARRERWKQR LIKYQAFDAS TYDQGVYDFL TIEDLNYGFD
     RFWLRRKMRN HQIRFRLFPG PWMRSLKKQL NNPANPSLET STKAASGPRV EFFRILFEQF
     YHPNFHDRAA MQTNPAEARN KFISTSPLAS TESKKALNST FSLGNINNSS TGIEGLVLTN
     TQATLLPTDL QTKRTIKPGL IYTNSALRKF VRNVNTRLNL KLLNSKETNL TTKYKSQFIY
     SKRWKSIFSK IQPLQNGTTR KSYQLFRNVA KQILVTPDAK SLKLITINQK LSLKERKLLE
     LRTQYNNNST LTTTAPLTLV RPLNVYLQKE EAFKRKLRYY GTMPMRKLTV GNQAPYFKAL
     MKRGFYYYKP TLRWRKTLYV ASLRRGFRKK SRKQRILVMP SNQQNFNNTL DNTKTNINQN
     NLANPLGGNE VPMYGADGEN SLITKPTHSY TVLGKRASRY RHQIYKDVLQ HWYYTPFNRL
     LMKFDVDAFI NRQPKSHFLT KNEERALHIR RFLLSEHYDT LRWYTYMQHY KTMKTNIGGT
     KSFANRAYNQ QFQGTFKKIR HLFAITPKQG DFYTLKFDQP LYNDNKLKDN LYFHEELLTD
     YYNGTNLQTN QTSNISVNST TTFIDNSLRT TQLPVPSSSF DIVNQSSTLI GLTTMQNALR
     KNVVESTLTS LNSDGEAATS QPKLNFVYSE LFVKLIKECK KRIHDQTFLK NYITHRIEKR
     EQLNQEQTKE LNKRLEKLKV WLNSDKGSIS KLQNTPVQDP NISSPDKVLT TAMQKAVNES
     ISLSGIMPSD KIKTTYGNLT NAYTIKTENA ILTKLNVINQ LTNNETTTQK NTLIKSIGVN
     KIQTVLQTII TNFKSSLYNQ TQLLRVKTDK DLQWWRTKQR VITKRKSARK RDRFKKQIAV
     VNKKLAALSK KVETEKSNLY QTLYGNYEIS DYLLRNVPTG SSAVIDSTVL RKKQDNQAYL
     PKETNNVQFN SFVDSNNNVW QTFFAKKLRK KISSKGRRYR SLSLARYLTA TRKPRLVGLD
     NLTKIDNITT LQGAFITKEE KQDSLNLTIQ RKQELTNSLK KSQIKKRSRH SWKKRSRHQF
     SRNHYKYRKR HTHGNGKLRV MNKKLKKFKA TNELRQWWWN SFLPRYLSNL QVNNSTLTNK
     NVSFKPLSNT NSVPSTNMAS PTTSRNLLDN LNSSNQISTS ASMNQNIVTE SVKVETNQVY
     LPEGEKSFDI TSMTTTLPFY AGWDESLKKF VVTNRLLSRR DAGLSVNNNP QEINFTNPPI
     QGLNEGSFLY WQTEMPFNSY NIDQFITTNQ SFYAPLGWRR FEFRHSILKT WVNNTKAGNN
     NIKKKTLIIS LKNLQPLKSS QQKQNQIKTK KLVARRIKKR YKLLKQMPNQ LMYSPTGPLL
     TEVLPSHYIS VFDQQYRLPR NRYLKRNPLK TLKKTTLLAL MDSSKQTNGV NKEFTLRKRV
     KPRRKYHRKR FIKKDGLIFP RRTKFNTNTT LTGNALITNN VNSIEEDDLR WRPSSRTKQK
     RKDNTRSSAA SKTKSNKRVK TNPLRLRQLR RREFQQVLKP LQRYIPQNGG FTWPGDYLRL
     EIVEMPKLKS INIKKTSLKQ KINVQPVGIM PRKYLIEKHN IKVLKKKLSQ AYSTQQLTKV
     VQEYKNLIQN SPPAI