CAX1A_ORYSJ
ID CAX1A_ORYSJ Reviewed; 451 AA.
AC Q769E5; Q0JLY3; Q8S3C7;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Vacuolar cation/proton exchanger 1a;
DE AltName: Full=Ca(2+)/H(+) exchanger 1a;
DE AltName: Full=OsCAX1a;
GN Name=CAX1a; OrderedLocusNames=Os01g0557500, LOC_Os01g37690;
GN ORFNames=B1144D11.14, OJ1014_G12.40;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ACTIVITY REGULATION,
RP BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, DOMAIN, AND
RP MUTAGENESIS OF GLY-128; ASN-129; GLU-132; LYS-144; LYS-149; GLY-154;
RP ASN-326; GLU-329; HIS-330; LYS-341; GLY-351; SER-352 AND GLN-355.
RC STRAIN=cv. Nipponbare;
RX PubMed=14561741; DOI=10.1074/jbc.m309726200;
RA Kamiya T., Maeshima M.;
RT "Residues in internal repeats of the rice cation/H+ exchanger are involved
RT in the transport and selection of cations.";
RL J. Biol. Chem. 279:812-819(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Shen G.M., Wang X.C.;
RL Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [7]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=16043430; DOI=10.1093/pcp/pci173;
RA Kamiya T., Akahori T., Maeshima M.;
RT "Expression profile of the genes for rice cation/h+ exchanger family and
RT functional analysis in yeast.";
RL Plant Cell Physiol. 46:1735-1740(2005).
CC -!- FUNCTION: Vacuolar cation/proton exchanger (CAX). Translocates Ca(2+)
CC and other metal ions into vacuoles using the proton gradient formed by
CC H(+)-ATPase and H(+)-pyrophosphatase. {ECO:0000269|PubMed:14561741,
CC ECO:0000269|PubMed:16043430}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=9.5 uM for Ca(2+) (at pH 7.2 and 22 degrees Celsius)
CC {ECO:0000269|PubMed:14561741};
CC Vmax=3.8 nmol/min/mg enzyme with Ca(2+) as ion
CC {ECO:0000269|PubMed:14561741};
CC Note=Measured in vacuolar yeast membrane vesicles.;
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000305|PubMed:14561741};
CC Multi-pass membrane protein {ECO:0000305|PubMed:14561741}.
CC Note=Tonoplast.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q769E5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q769E5-2; Sequence=VSP_015366, VSP_015367, VSP_015368;
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:16043430}.
CC -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19)
CC family. Cation/proton exchanger (CAX) subfamily. {ECO:0000305}.
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DR EMBL; AB112656; BAD06218.1; -; mRNA.
DR EMBL; AF489111; AAM03123.1; -; mRNA.
DR EMBL; AP003372; BAD87498.1; -; Genomic_DNA.
DR EMBL; AP003443; BAD87649.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF05245.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS72688.1; -; Genomic_DNA.
DR RefSeq; XP_015620208.1; XM_015764722.1. [Q769E5-1]
DR AlphaFoldDB; Q769E5; -.
DR SMR; Q769E5; -.
DR STRING; 4530.OS01T0557500-01; -.
DR TCDB; 2.A.19.2.10; the ca(2+):cation antiporter (caca) family.
DR PaxDb; Q769E5; -.
DR PRIDE; Q769E5; -.
DR EnsemblPlants; Os01t0557500-01; Os01t0557500-01; Os01g0557500. [Q769E5-1]
DR GeneID; 4324573; -.
DR Gramene; Os01t0557500-01; Os01t0557500-01; Os01g0557500. [Q769E5-1]
DR KEGG; osa:4324573; -.
DR eggNOG; KOG1397; Eukaryota.
DR HOGENOM; CLU_008721_2_0_1; -.
DR InParanoid; Q769E5; -.
DR OMA; VLLMMMY; -.
DR OrthoDB; 727723at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR Genevisible; Q769E5; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009705; C:plant-type vacuole membrane; IBA:GO_Central.
DR GO; GO:0015369; F:calcium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IBA:GO_Central.
DR Gene3D; 1.20.1420.30; -; 1.
DR InterPro; IPR004713; CaH_exchang.
DR InterPro; IPR004798; CAX-like.
DR InterPro; IPR004837; NaCa_Exmemb.
DR InterPro; IPR044880; NCX_ion-bd_dom_sf.
DR PANTHER; PTHR31503; PTHR31503; 1.
DR Pfam; PF01699; Na_Ca_ex; 2.
DR TIGRFAMs; TIGR00378; cax; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Antiport; Calcium; Calcium transport; Ion transport;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport; Vacuole.
FT CHAIN 1..451
FT /note="Vacuolar cation/proton exchanger 1a"
FT /id="PRO_0000209497"
FT TOPO_DOM 1..58
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 80..86
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 108..120
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 142..153
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 154..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 175..191
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 192..212
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 213..228
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 229..249
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 250..273
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 295..317
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 318..338
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 339..352
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 353..373
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 374..378
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 379..399
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 400..404
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 405..425
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 426..451
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT REGION 9..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 128..163
FT /note="Cation selection"
FT /evidence="ECO:0000255"
FT REGION 325..360
FT /note="Cation selection"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..110
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_015366"
FT VAR_SEQ 111..118
FT /note="IALYTGPT -> MMVYAWGA (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_015367"
FT VAR_SEQ 430..451
FT /note="GHANSNGALLDVPTGSMSVQAA -> ASGTAVSLYVDYHIFVMISSIWCDRV
FT ANLSDSWNA (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_015368"
FT MUTAGEN 128
FT /note="G->A: Reduced transport of Ca(2+) and loss of
FT transport of Mn(2+)."
FT /evidence="ECO:0000269|PubMed:14561741"
FT MUTAGEN 129
FT /note="N->A: Loss of transport of Ca(2+) and Mn(2+)."
FT /evidence="ECO:0000269|PubMed:14561741"
FT MUTAGEN 132
FT /note="E->D: Reduced transport of Ca(2+) and loss of
FT transport of Mn(2+)."
FT /evidence="ECO:0000269|PubMed:14561741"
FT MUTAGEN 132
FT /note="E->Q: Loss of transport of Ca(2+) and Mn(2+)."
FT /evidence="ECO:0000269|PubMed:14561741"
FT MUTAGEN 144
FT /note="K->A: Reduced transport of Ca(2+) and Mn(2+)."
FT /evidence="ECO:0000269|PubMed:14561741"
FT MUTAGEN 149
FT /note="K->A: Reduced transport of Ca(2+) and Mn(2+)."
FT /evidence="ECO:0000269|PubMed:14561741"
FT MUTAGEN 154
FT /note="G->A: Loss of transport of Ca(2+) and Mn(2+)."
FT /evidence="ECO:0000269|PubMed:14561741"
FT MUTAGEN 326
FT /note="N->A: Reduced transport of Ca(2+) and loss of
FT transport of Mn(2+)."
FT /evidence="ECO:0000269|PubMed:14561741"
FT MUTAGEN 329
FT /note="E->D,Q: Loss of transport of Ca(2+) and Mn(2+)."
FT /evidence="ECO:0000269|PubMed:14561741"
FT MUTAGEN 330
FT /note="H->A,R: Loss of transport of Ca(2+) and Mn(2+)."
FT /evidence="ECO:0000269|PubMed:14561741"
FT MUTAGEN 341
FT /note="K->A: Reduced transport of Ca(2+) and Mn(2+)."
FT /evidence="ECO:0000269|PubMed:14561741"
FT MUTAGEN 351
FT /note="G->A: Reduced transport of Ca(2+) and Mn(2+)."
FT /evidence="ECO:0000269|PubMed:14561741"
FT MUTAGEN 352
FT /note="S->A: Reduced transport of Ca(2+) and loss of
FT transport of Mn(2+)."
FT /evidence="ECO:0000269|PubMed:14561741"
FT MUTAGEN 355
FT /note="Q->A: Reduced transport of Ca(2+) and loss of
FT transport of Mn(2+)."
FT /evidence="ECO:0000269|PubMed:14561741"
SQ SEQUENCE 451 AA; 47707 MW; 6CD431E3D6796662 CRC64;
MEAAAAMEAG RKLAARHPHG RSRTAHNMSS SSLRKKSDAA LVRKVPVAPL RPLLANLQEV
FLATKLAVLF PAVPLAIAAQ CFRFDQVWVF ALSLLGLIPL AERVSFLTEQ IALYTGPTVG
GLLNATCGNA TELIIALFAL LKGKIEVVKC SLLGSVLSNL LLVLGTSLFC GGVVNLGARQ
PYDRNQSDVS TALLFLAVLC HSAPLLLRYA VAAGEHSVSA TSAAASLDLS RACSFVMLAS
YVAYLFFQLK THRQLFEPQE VDGGDAGDDD EEPALGFASA LFWLALMTAV ISVLSEYVVG
TIEPTSQSWG LSVSFISIIL LPIVGNAAEH AGAIIFALKN KLDITLGVAL GSATQISMFV
VPLSVLVAWI MGVQMDLDFK LLETGSLFMA VLVTAFTLQD GTSHYLKGIL LLLCYIVIGA
CFFVARQPAG HANSNGALLD VPTGSMSVQA A
