CAX2_ORYSJ
ID CAX2_ORYSJ Reviewed; 437 AA.
AC Q5KQN0; A0A0P0VZA2; Q10K42; Q5KQN1; Q5KTQ8; Q84MW3;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 30-AUG-2005, sequence version 2.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Vacuolar cation/proton exchanger 2;
DE AltName: Full=Ca(2+)/H(+) exchanger 2;
DE AltName: Full=OsCAX2;
GN Name=CAX2; OrderedLocusNames=Os03g0397400, LOC_Os03g27960;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 23-437, FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=16043430; DOI=10.1093/pcp/pci173;
RA Kamiya T., Akahori T., Maeshima M.;
RT "Expression profile of the genes for rice cation/h+ exchanger family and
RT functional analysis in yeast.";
RL Plant Cell Physiol. 46:1735-1740(2005).
CC -!- FUNCTION: Vacuolar cation/proton exchanger (CAX). Translocates Ca(2+)
CC and other metal ions into vacuoles using the proton gradient formed by
CC H(+)-ATPase and H(+)-pyrophosphatase. {ECO:0000269|PubMed:16043430}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}. Note=Tonoplast.
CC -!- TISSUE SPECIFICITY: Expressed in roots and shoots.
CC {ECO:0000269|PubMed:16043430}.
CC -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19)
CC family. Cation/proton exchanger (CAX) subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAP12929.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAW39030.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAD83662.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC091787; AAP12929.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC091787; AAW39029.1; -; Genomic_DNA.
DR EMBL; AC091787; AAW39030.1; ALT_SEQ; Genomic_DNA.
DR EMBL; DP000009; ABF96432.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF12215.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS84573.1; -; Genomic_DNA.
DR EMBL; AK069928; BAG91683.1; -; mRNA.
DR EMBL; AB112772; BAD83662.1; ALT_INIT; mRNA.
DR RefSeq; XP_015627891.1; XM_015772405.1.
DR RefSeq; XP_015627892.1; XM_015772406.1.
DR AlphaFoldDB; Q5KQN0; -.
DR SMR; Q5KQN0; -.
DR STRING; 4530.OS03T0397400-01; -.
DR PaxDb; Q5KQN0; -.
DR PRIDE; Q5KQN0; -.
DR EnsemblPlants; Os03t0397400-01; Os03t0397400-01; Os03g0397400.
DR GeneID; 4333044; -.
DR Gramene; Os03t0397400-01; Os03t0397400-01; Os03g0397400.
DR KEGG; osa:4333044; -.
DR eggNOG; KOG1397; Eukaryota.
DR HOGENOM; CLU_008721_2_0_1; -.
DR InParanoid; Q5KQN0; -.
DR OMA; KMQALDF; -.
DR OrthoDB; 727723at2759; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; Q5KQN0; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009705; C:plant-type vacuole membrane; IBA:GO_Central.
DR GO; GO:0015369; F:calcium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IBA:GO_Central.
DR Gene3D; 1.20.1420.30; -; 2.
DR InterPro; IPR004713; CaH_exchang.
DR InterPro; IPR004798; CAX-like.
DR InterPro; IPR004837; NaCa_Exmemb.
DR InterPro; IPR044880; NCX_ion-bd_dom_sf.
DR PANTHER; PTHR31503; PTHR31503; 1.
DR Pfam; PF01699; Na_Ca_ex; 2.
DR TIGRFAMs; TIGR00378; cax; 1.
PE 2: Evidence at transcript level;
KW Antiport; Calcium; Calcium transport; Ion transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..437
FT /note="Vacuolar cation/proton exchanger 2"
FT /id="PRO_0000209500"
FT TOPO_DOM 1..65
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 66..86
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 87..88
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 110..126
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 148..161
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 162..182
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 183..194
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 216..228
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 229..249
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 250..281
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 303..310
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 332..352
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 353..373
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 374..379
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 380..400
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 401..408
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 409..429
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 430..437
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 136..171
FT /note="Cation selection"
FT /evidence="ECO:0000255"
FT REGION 328..363
FT /note="Cation selection"
FT /evidence="ECO:0000255"
SQ SEQUENCE 437 AA; 47404 MW; 2953D8B70B09A14D CRC64;
MMGAEKAEGM EELELEEGGG SPSPSPMTAA GKMQALDFEH IGSLAAVAES LSTGSKWRRA
LTSVRVVILQ AKINVLLPFG PLAVMLHYLS ANHQGWVFLF SLIGITPLAE RLGYATEQLA
LYTGPTIGGL LNATFGNATE MIISLYALKN GMIRVVQQSL LGSILSNMLL VLGCAFFAGG
LVHPSRDQVF NKASAVVNSG LLLMAVLGLM FPAVLHFTHS EVQYGKSEVS LSRFSSCIML
VAYASYLFFQ LKSQRSLYSP IGEQEEEVTE DEEEEKEITQ GEAICWLFVL TIWISILSGY
LVDAIQGASE SLNMPVAFIS VILLPIVGNA AEHASAIMFA MKDKLDITLG VAIGSSTQIS
MFVIPFCVVI GWIMGQQMDL NFQLFETATL FITVLVVAFM LQEGTSNYFK GLMLILCYLI
VAASFFVHVD PDSSNNK
