ID   YCG6_SCHPO              Reviewed;         413 AA.
AC   O74451;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 2.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=Putative tRNA pseudouridine synthase C16C4.06c;
DE            EC=5.4.99.-;
GN   ORFNames=SPCC16C4.06c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in tRNA = a pseudouridine in tRNA;
CC         Xref=Rhea:RHEA:54572, Rhea:RHEA-COMP:13339, Rhea:RHEA-COMP:13934,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CU329672; CAA20745.2; -; Genomic_DNA.
DR   PIR; T41096; T41096.
DR   RefSeq; NP_587916.1; NM_001022907.2.
DR   AlphaFoldDB; O74451; -.
DR   SMR; O74451; -.
DR   BioGRID; 275925; 13.
DR   STRING; 4896.SPCC16C4.06c.1; -.
DR   MaxQB; O74451; -.
DR   PaxDb; O74451; -.
DR   PRIDE; O74451; -.
DR   EnsemblFungi; SPCC16C4.06c.1; SPCC16C4.06c.1:pep; SPCC16C4.06c.
DR   PomBase; SPCC16C4.06c; -.
DR   VEuPathDB; FungiDB:SPCC16C4.06c; -.
DR   eggNOG; KOG2553; Eukaryota.
DR   HOGENOM; CLU_021971_4_0_1; -.
DR   InParanoid; O74451; -.
DR   OMA; SAPNDAM; -.
DR   PhylomeDB; O74451; -.
DR   PRO; PR:O74451; -.
DR   Proteomes; UP000002485; Chromosome III.
DR   GO; GO:0005737; C:cytoplasm; HDA:PomBase.
DR   GO; GO:0005634; C:nucleus; ISO:PomBase.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009982; F:pseudouridine synthase activity; ISO:PomBase.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:1990481; P:mRNA pseudouridine synthesis; IBA:GO_Central.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; ISO:PomBase.
DR   CDD; cd02568; PseudoU_synth_PUS1_PUS2; 1.
DR   Gene3D; 3.30.70.580; -; 1.
DR   Gene3D; 3.30.70.660; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR001406; PsdUridine_synth_TruA.
DR   InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR   InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR   InterPro; IPR041708; PUS1/PUS2-like.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   PANTHER; PTHR11142; PTHR11142; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00071; hisT_truA; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Isomerase; Lyase; Nucleus; Reference proteome; tRNA processing.
FT   CHAIN           1..413
FT                   /note="Putative tRNA pseudouridine synthase C16C4.06c"
FT                   /id="PRO_0000316610"
FT   ACT_SITE        96
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   BINDING         154
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   413 AA;  47498 MW;  235487349F60ECF1 CRC64;
     MHSLLRPSRF SANQSVLCLL QRHRVFFSSI SVKPRKRMVY CVVSYRGTGF AGLQYNANVK
     TIQDTLFQAF ARVGAVVQVN ADSPKKIRMC SAARTDKGVH AIVNVLGLKV LDNQPLPHVV
     NLVNDILPPC IRVWKMARTF NSFSPHTVCD SRVYEYWLPV SSLLTPRPCT LEAYVIAKAS
     EKAFPINETL SHLANLSKQD CVANSLSEPF RLSKPKLDFL KHACTMFRGT HRFHSYTTEK
     GFSDASSRRF LLDVRVDNLH IDKLNRQWVK LIFHGQSFMK HQIRKMVGIL IHLTRTGWNA
     QVLLNTFNNS YRIRIPRAPA EFLLLNQPIF QAFNKKCSRF DHEPVEWSCA QKGIDQFAHS
     FLRTPMFDCF ISSESFQSFF ILQKQFQLFQ DLALLTSFDF LEPSSRRDIL GEK