CAX4_ARATH
ID CAX4_ARATH Reviewed; 446 AA.
AC Q945S5; F4K9E5; Q9M025;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Vacuolar cation/proton exchanger 4;
DE Short=AtCAX4;
DE AltName: Full=Ca(2+)/H(+) antiporter CAX4;
DE AltName: Full=Ca(2+)/H(+) exchanger 4;
DE AltName: Full=Protein CATION EXCHANGER 4;
GN Name=CAX4; OrderedLocusNames=At5g01490; ORFNames=F7A7.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, INDUCTION, AND MUTAGENESIS OF 88-VAL--ARG-96.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=11950973; DOI=10.1104/pp.010857;
RA Cheng N.-H., Pittman J.K., Shigaki T., Hirschi K.D.;
RT "Characterization of CAX4, an Arabidopsis H(+)/cation antiporter.";
RL Plant Physiol. 128:1245-1254(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
RN [5]
RP BIOTECHNOLOGY.
RX PubMed=16244156; DOI=10.1104/pp.105.066266;
RA Park S., Cheng N.-H., Pittman J.K., Yoo K.S., Park J., Smith R.H.,
RA Hirschi K.D.;
RT "Increased calcium levels and prolonged shelf life in tomatoes expressing
RT Arabidopsis H+/Ca2+ transporters.";
RL Plant Physiol. 139:1194-1206(2005).
RN [6]
RP FUNCTION.
RX PubMed=16845524; DOI=10.1007/s00425-006-0352-7;
RA Koren'kov V., Park S., Cheng N.-H., Sreevidya C., Lachmansingh J.,
RA Morris J., Hirschi K., Wagner G.J.;
RT "Enhanced Cd(2+)-selective root-tonoplast-transport in tobaccos expressing
RT Arabidopsis cation exchangers.";
RL Planta 225:403-411(2007).
CC -!- FUNCTION: Vacuolar cation/proton exchanger (CAX). Translocates Ca(2+)
CC and other metal ions into vacuoles using the proton gradient formed by
CC H(+)-ATPase and H(+)-pyrophosphatase. Cation selectivity transport in
CC tobacco root tonoplast vesicles is Cd(2+)>Zn(2+)>>Ca(2+)>>>Mn(2+).
CC {ECO:0000269|PubMed:16845524}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000305|PubMed:11950973};
CC Multi-pass membrane protein {ECO:0000305|PubMed:11950973}.
CC Note=Tonoplast.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q945S5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q945S5-2; Sequence=VSP_042221;
CC -!- TISSUE SPECIFICITY: Expressed at low levels in all tissues.
CC {ECO:0000269|PubMed:11950973}.
CC -!- INDUCTION: Slightly induced by Mn(2+), Na(+) and Ni(2+).
CC {ECO:0000269|PubMed:11950973}.
CC -!- BIOTECHNOLOGY: CAX4 expression in tomato increases calcium content and
CC prolonges shelf life and may serve as an alternative to the application
CC of CaCl(2) used to extend shelf life in numerous agricultural
CC commodities. {ECO:0000269|PubMed:16244156}.
CC -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19)
CC family. Cation/proton exchanger (CAX) subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB82265.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF409107; AAK97656.1; -; mRNA.
DR EMBL; AL161946; CAB82265.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED90352.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM69424.1; -; Genomic_DNA.
DR PIR; T48170; T48170.
DR RefSeq; NP_001331104.1; NM_001342594.1. [Q945S5-2]
DR RefSeq; NP_568091.2; NM_120227.4. [Q945S5-2]
DR AlphaFoldDB; Q945S5; -.
DR SMR; Q945S5; -.
DR STRING; 3702.AT5G01490.1; -.
DR TCDB; 2.A.19.2.9; the ca(2+):cation antiporter (caca) family.
DR iPTMnet; Q945S5; -.
DR PaxDb; Q945S5; -.
DR PRIDE; Q945S5; -.
DR ProteomicsDB; 222790; -. [Q945S5-1]
DR EnsemblPlants; AT5G01490.1; AT5G01490.1; AT5G01490. [Q945S5-2]
DR EnsemblPlants; AT5G01490.2; AT5G01490.2; AT5G01490. [Q945S5-2]
DR GeneID; 831754; -.
DR Gramene; AT5G01490.1; AT5G01490.1; AT5G01490. [Q945S5-2]
DR Gramene; AT5G01490.2; AT5G01490.2; AT5G01490. [Q945S5-2]
DR KEGG; ath:AT5G01490; -.
DR Araport; AT5G01490; -.
DR TAIR; locus:2149720; AT5G01490.
DR eggNOG; KOG1397; Eukaryota.
DR HOGENOM; CLU_008721_2_0_1; -.
DR InParanoid; Q945S5; -.
DR OrthoDB; 727723at2759; -.
DR PhylomeDB; Q945S5; -.
DR PRO; PR:Q945S5; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q945S5; baseline and differential.
DR Genevisible; Q945S5; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009705; C:plant-type vacuole membrane; IDA:TAIR.
DR GO; GO:0015368; F:calcium:cation antiporter activity; IDA:TAIR.
DR GO; GO:0015369; F:calcium:proton antiporter activity; IMP:TAIR.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IBA:GO_Central.
DR GO; GO:0009733; P:response to auxin; IMP:TAIR.
DR GO; GO:0048364; P:root development; IMP:TAIR.
DR Gene3D; 1.20.1420.30; -; 2.
DR InterPro; IPR004713; CaH_exchang.
DR InterPro; IPR004798; CAX-like.
DR InterPro; IPR004837; NaCa_Exmemb.
DR InterPro; IPR044880; NCX_ion-bd_dom_sf.
DR PANTHER; PTHR31503; PTHR31503; 1.
DR Pfam; PF01699; Na_Ca_ex; 2.
DR TIGRFAMs; TIGR00378; cax; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Antiport; Calcium; Calcium transport; Ion transport;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport; Vacuole.
FT CHAIN 1..446
FT /note="Vacuolar cation/proton exchanger 4"
FT /id="PRO_0000270153"
FT TOPO_DOM 1..69
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 91..96
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 97..117
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 118..128
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 129..149
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 150..162
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..183
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 184..196
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 218..238
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 239..259
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 260..285
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 286..306
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 307..319
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 341..359
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 360..380
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 381..388
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 389..409
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 410..416
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 417..437
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 438..446
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 138..173
FT /note="Cation selection"
FT /evidence="ECO:0000255"
FT REGION 337..372
FT /note="Cation selection"
FT /evidence="ECO:0000255"
FT COMPBIAS 1..17
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 442..446
FT /note="SKLLF -> TETNTTNHTITKR (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_042221"
FT MUTAGEN 88..96
FT /note="VAHRYDCPR->ICTYCGVSQ: Facilitates Ca(2+)/H(+)
FT exchange activity."
FT /evidence="ECO:0000269|PubMed:11950973"
FT CONFLICT 13
FT /note="S -> C (in Ref. 1; AAK97656)"
FT /evidence="ECO:0000305"
FT CONFLICT 273
FT /note="H -> Y (in Ref. 1; AAK97656)"
FT /evidence="ECO:0000305"
FT CONFLICT 399..404
FT /note="FALSIL -> LAMSII (in Ref. 1; AAK97656)"
FT /evidence="ECO:0000305"
FT CONFLICT 428
FT /note="V -> F (in Ref. 1; AAK97656)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 446 AA; 48699 MW; C8C69E511CC37B5B CRC64;
MSSISTESSS NLSLLENGGG GSDKPTAETS RRVRRTVSAS SLIRKRSDLK LISRVRWEFM
RRILTNLQEV LLGTKLFILF PAVPLAVVAH RYDCPRAWVF ALSLLGLTPL AERISFLTEQ
IAFHTGPTVG GLMNATCGNA TEMIIAILAV GQRKMRIVKL SLLGSILSNL LFVLGTSLFL
GGISNLRKHQ SFDPRQGDMN SMLLYLALLC QTLPMIMRFT MEAEEYDGSD VVVLSRASSF
VMLIAYLAFL IFHLFSSHLS PPPPPLPQRE DVHDDDVSDK EEEGAVIGMW SAIFWLIIMT
LLVALLSDYL VSTIQDAADS WGLSVGFIGI ILLPIVGNAA EHAGAVIFAF RNKLDITLGI
ALGSATQIAL FVVPVTVLVA WTMGIEMDLN FNLLETACFA LSILVTSLVL QDGTSNYMKG
LVLLLCYVVI AACFFVSNSP SSKLLF
