CAX6_ARATH
ID CAX6_ARATH Reviewed; 448 AA.
AC Q9LFZ8; F4I1W3;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 22-SEP-2009, sequence version 3.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Putative vacuolar cation/proton exchanger 6;
DE AltName: Full=Ca(2+)/H(+) antiporter CAX6;
DE AltName: Full=Ca(2+)/H(+) exchanger 6;
DE AltName: Full=Protein CATION EXCHANGER 6;
GN Name=CAX6; OrderedLocusNames=At1g55720; ORFNames=F20N2.13, F20N2.23;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
CC -!- FUNCTION: Vacuolar cation/proton exchanger (CAX). Translocates Ca(2+)
CC and other metal ions into vacuoles using the proton gradient formed by
CC H(+)-ATPase and H(+)-pyrophosphatase (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}. Note=Tonoplast.
CC -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19)
CC family. Cation/proton exchanger (CAX) subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79505.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AEE33289.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC002328; AAF79505.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33289.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; ANM58202.1; -; Genomic_DNA.
DR RefSeq; NP_001320656.1; NM_001333724.1.
DR RefSeq; NP_175968.4; NM_104448.4.
DR AlphaFoldDB; Q9LFZ8; -.
DR SMR; Q9LFZ8; -.
DR STRING; 3702.AT1G55720.1; -.
DR PaxDb; Q9LFZ8; -.
DR PRIDE; Q9LFZ8; -.
DR EnsemblPlants; AT1G55720.2; AT1G55720.2; AT1G55720.
DR GeneID; 842021; -.
DR Gramene; AT1G55720.2; AT1G55720.2; AT1G55720.
DR KEGG; ath:AT1G55720; -.
DR Araport; AT1G55720; -.
DR eggNOG; KOG1397; Eukaryota.
DR HOGENOM; CLU_008721_2_1_1; -.
DR InParanoid; Q9LFZ8; -.
DR OMA; HHGWVFL; -.
DR OrthoDB; 727723at2759; -.
DR PhylomeDB; Q9LFZ8; -.
DR PRO; PR:Q9LFZ8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LFZ8; baseline and differential.
DR Genevisible; Q9LFZ8; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009705; C:plant-type vacuole membrane; IBA:GO_Central.
DR GO; GO:0015369; F:calcium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IBA:GO_Central.
DR Gene3D; 1.20.1420.30; -; 2.
DR InterPro; IPR004713; CaH_exchang.
DR InterPro; IPR004798; CAX-like.
DR InterPro; IPR004837; NaCa_Exmemb.
DR InterPro; IPR044880; NCX_ion-bd_dom_sf.
DR PANTHER; PTHR31503; PTHR31503; 1.
DR Pfam; PF01699; Na_Ca_ex; 2.
DR TIGRFAMs; TIGR00378; cax; 1.
PE 3: Inferred from homology;
KW Antiport; Calcium; Calcium transport; Ion transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..448
FT /note="Putative vacuolar cation/proton exchanger 6"
FT /id="PRO_0000270155"
FT TOPO_DOM 31..81
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 103..109
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 110..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 131..141
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 163..178
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 200..209
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..230
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 231..243
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 265..295
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 317..334
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 335..355
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 356..363
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 364..384
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 385..393
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 394..414
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 415..425
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 426..446
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 447..448
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 150..185
FT /note="Cation selection"
FT /evidence="ECO:0000255"
FT REGION 341..376
FT /note="Cation selection"
FT /evidence="ECO:0000255"
SQ SEQUENCE 448 AA; 49583 MW; 2621B960A4E493DC CRC64;
MYMELYLMFS NFFKSIRWCK VPAFMQAKVE MGLVNEVELK SLLEQETDSP QTNAASLMEQ
GSLRERRAKA PRNSVVQSFK IVILSNKLNL LLPFGPLAIL VHYLTDNKGW FFLLSLVGIT
PLAERLGYAT EQLSCYTGAT VGGLLNATFG NVIELIISII ALKNGMIRVV QLTLLGSILS
NILLVLGCAF FCGGLVFPGK DQVFDKRNAV VSSGMLLMAV MGLLFPTFLH YTHSEVHAGS
SELALSRFIS CIMLVAYAAY LFFQLKSQPS FYTEKTNQNE ETSNDDEDPE ISKWEAIIWL
SIFTAWVSLL SGYLVDAIEG TSVSWKIPIS FISVILLPIV GNAAEHAGAI MFAMKDKLDL
SLGVAIGSSI QISMFAVPFC VVIGWMMGAQ MDLNLQLFET ATLLITVIVV AFFLQLEGTS
NYFKRLMLIL CYLIVAASFF VHEDPHQG
