CAX_APHHA
ID CAX_APHHA Reviewed; 373 AA.
AC Q75XW3;
DT 19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 36.
DE RecName: Full=Ca(2+)/H(+) antiporter;
DE AltName: Full=ApCAX;
OS Aphanothece halophytica.
OC Bacteria; Cyanobacteria; Oscillatoriophycideae; Chroococcales;
OC Aphanothecaceae; Aphanothece.
OX NCBI_TaxID=72020;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES,
RP SUBCELLULAR LOCATION, AND MUTAGENESIS OF GLU-74 AND GLU-324.
RX PubMed=14559898; DOI=10.1074/jbc.m310282200;
RA Waditee R., Hossain G.S., Tanaka Y., Nakamura T., Shikata M., Takano J.,
RA Takabe T., Takabe T.;
RT "Isolation and functional characterization of Ca2+/H+ antiporters from
RT cyanobacteria.";
RL J. Biol. Chem. 279:4330-4338(2004).
CC -!- FUNCTION: Ca(+)/H(+) antiporter that extrudes calcium in exchange for
CC external protons. Plays an important role in salt tolerance. Does not
CC transport sodium or lithium. {ECO:0000269|PubMed:14559898}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 8.8. {ECO:0000269|PubMed:14559898};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:14559898}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:14559898}.
CC -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19)
CC family. Cation/proton exchanger (CAX) subfamily. {ECO:0000305}.
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DR EMBL; AB120300; BAD08687.1; -; Genomic_DNA.
DR AlphaFoldDB; Q75XW3; -.
DR SMR; Q75XW3; -.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:UniProtKB.
DR GO; GO:0015369; F:calcium:proton antiporter activity; IDA:UniProtKB.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IDA:UniProtKB.
DR Gene3D; 1.20.1420.30; -; 1.
DR InterPro; IPR004713; CaH_exchang.
DR InterPro; IPR004798; CAX-like.
DR InterPro; IPR004837; NaCa_Exmemb.
DR InterPro; IPR044880; NCX_ion-bd_dom_sf.
DR PANTHER; PTHR31503; PTHR31503; 1.
DR Pfam; PF01699; Na_Ca_ex; 2.
DR TIGRFAMs; TIGR00378; cax; 1.
PE 1: Evidence at protein level;
KW Antiport; Calcium; Calcium transport; Cell inner membrane; Cell membrane;
KW Ion transport; Membrane; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..373
FT /note="Ca(2+)/H(+) antiporter"
FT /id="PRO_0000425710"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 29..49
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 61..81
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 162..182
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..240
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 249..269
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 291..311
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 318..338
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 349..369
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MUTAGEN 74
FT /note="E->D,H: Lack of activity."
FT /evidence="ECO:0000269|PubMed:14559898"
FT MUTAGEN 74
FT /note="E->Q: 2-fold decrease in activity."
FT /evidence="ECO:0000269|PubMed:14559898"
FT MUTAGEN 324
FT /note="E->D,H,Q: Lack of activity."
FT /evidence="ECO:0000269|PubMed:14559898"
SQ SEQUENCE 373 AA; 39713 MW; 7FCF5F6DBDB86663 CRC64;
MLNKNTIFFG LLLFIPISLL GHWLHWDEVS IFLTASLAII PLAAFMGEAT EEIAIVVGPT
LGGLLNATFG NATELILAFI ALKSGLISVV KATITGSIIS NLLLVMGFAM LLGGLRYKEQ
VFQSEVARVN ASSMNLAVIA ILLPTAVEHT SNGIGEETLQ TLSVAVAIVL IIVYGLTLLF
SMKTHSYLCE VGEIDQQSSS AMESGDKIPE KKPEDVNLWF WLGILLVVTI TVAIESELLV
NSLESATESL GLTALFTGVI LLPVIGNAAE HFTAVTVAMK NKMDLSLSVA VGSTLQIALF
VAPVLVIAGW IMGQPMDLDF NPFELVAVAV SVLIANSISS DGKSNWLEGS LLLATYIVIG
LAFFFHPVVP EIG
