YCIB_SHIFL
ID YCIB_SHIFL Reviewed; 179 AA.
AC P95745;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2003, sequence version 2.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Inner membrane-spanning protein YciB {ECO:0000255|HAMAP-Rule:MF_00189};
GN Name=yciB {ECO:0000255|HAMAP-Rule:MF_00189};
GN Synonyms=ispA {ECO:0000303|PubMed:8830250};
GN OrderedLocusNames=SF1257, S1343;
OS Shigella flexneri.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=YSH6000 / Serotype 2a;
RX PubMed=8830250; DOI=10.1046/j.1365-2958.1996.405941.x;
RA Mac Siomoin R.A., Nakata N., Murai T., Yoshikawa M., Tsuji H., Sasakawa C.;
RT "Identification and characterization of ispA, a Shigella flexneri
RT chromosomal gene essential for normal in vivo cell division and
RT intracellular spreading.";
RL Mol. Microbiol. 19:599-609(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT "Complete genome sequence and comparative genomics of Shigella flexneri
RT serotype 2a strain 2457T.";
RL Infect. Immun. 71:2775-2786(2003).
RN [4]
RP SUBCELLULAR LOCATION, AND FUNCTION IN VIRULENCE.
RX PubMed=9746567; DOI=10.1128/iai.66.10.4700-4710.1998;
RA Hong M., Gleason Y., Wyckoff E.E., Payne S.M.;
RT "Identification of two Shigella flexneri chromosomal loci involved in
RT intercellular spreading.";
RL Infect. Immun. 66:4700-4710(1998).
CC -!- FUNCTION: Plays a role in cell envelope biogenesis, maintenance of cell
CC envelope integrity and membrane homeostasis (By similarity). Essential
CC for normal cell division (PubMed:8830250). Involved in virulence
CC through intercellular spreading (PubMed:8830250, PubMed:9746567).
CC {ECO:0000255|HAMAP-Rule:MF_00189, ECO:0000269|PubMed:8830250,
CC ECO:0000269|PubMed:9746567}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_00189, ECO:0000269|PubMed:9746567}; Multi-pass membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_00189, ECO:0000269|PubMed:9746567}.
CC -!- SIMILARITY: Belongs to the YciB family. {ECO:0000255|HAMAP-
CC Rule:MF_00189}.
CC -!- CAUTION: Cannot be called ispA as this name is already used.
CC {ECO:0000305}.
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DR EMBL; D42071; BAA07668.1; -; Genomic_DNA.
DR EMBL; AE005674; AAN42870.1; -; Genomic_DNA.
DR EMBL; AE014073; AAP16755.1; -; Genomic_DNA.
DR PIR; S70898; S70898.
DR RefSeq; NP_707163.1; NC_004337.2.
DR RefSeq; WP_000808673.1; NZ_WPGW01000009.1.
DR AlphaFoldDB; P95745; -.
DR STRING; 198214.SF1257; -.
DR EnsemblBacteria; AAN42870; AAN42870; SF1257.
DR EnsemblBacteria; AAP16755; AAP16755; S1343.
DR GeneID; 1024246; -.
DR KEGG; sfl:SF1257; -.
DR KEGG; sft:NCTC1_01329; -.
DR KEGG; sfx:S1343; -.
DR PATRIC; fig|198214.7.peg.1477; -.
DR HOGENOM; CLU_089554_2_0_6; -.
DR OMA; AWVNFKL; -.
DR OrthoDB; 1886685at2; -.
DR Proteomes; UP000001006; Chromosome.
DR Proteomes; UP000002673; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00189; YciB; 1.
DR InterPro; IPR006008; YciB.
DR PANTHER; PTHR36917; PTHR36917; 1.
DR Pfam; PF04279; IspA; 1.
DR TIGRFAMs; TIGR00997; ispZ; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Virulence.
FT CHAIN 1..179
FT /note="Inner membrane-spanning protein YciB"
FT /id="PRO_0000206550"
FT TOPO_DOM 1..21
FT /note="Periplasmic"
FT /evidence="ECO:0000250|UniProtKB:P0A710"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00189"
FT TOPO_DOM 43..49
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P0A710"
FT TRANSMEM 50..70
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00189"
FT TOPO_DOM 71..75
FT /note="Periplasmic"
FT /evidence="ECO:0000250|UniProtKB:P0A710"
FT TRANSMEM 76..96
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00189"
FT TOPO_DOM 97..120
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P0A710"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00189"
FT TOPO_DOM 142..148
FT /note="Periplasmic"
FT /evidence="ECO:0000250|UniProtKB:P0A710"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00189"
FT TOPO_DOM 170..179
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P0A710"
FT CONFLICT 87
FT /note="G -> L (in Ref. 1; BAA07668)"
FT /evidence="ECO:0000305"
FT CONFLICT 124
FT /note="A -> T (in Ref. 1; BAA07668)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 179 AA; 20820 MW; 6A43E79DDBCC2AEF CRC64;
MKQFLDFLPL VVFFAFYKIY DIYAATAALI VATAIVLIYS WVRFRKVEKM ALITFVLVVV
FGGLTLFFHN DEFIKWKVTV IYALFAGALL VSQWVMKKPL IQRMLSKELT LPQPVWSKLN
LAWAVFFILC GLANIYIAFW LPQNIWVNFK VFGLTALTLI FTLLSGIYIY RHMPQEDKS
