ID   YCLE_BACSU              Reviewed;         281 AA.
AC   P94407; Q797P6;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 2.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=AB hydrolase superfamily protein YclE;
DE            EC=3.-.-.-;
GN   Name=yclE; OrderedLocusNames=BSU03660;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969502; DOI=10.1099/13500872-142-11-3047;
RA   Yamane K., Kumano M., Kurita K.;
RT   "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT   determination of the sequence of a 146 kb segment and identification of 113
RT   genes.";
RL   Microbiology 142:3047-3056(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 32 AND 91.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. {ECO:0000305}.
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DR   EMBL; D50453; BAA08999.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12160.2; -; Genomic_DNA.
DR   PIR; B69762; B69762.
DR   RefSeq; NP_388248.2; NC_000964.3.
DR   RefSeq; WP_003246662.1; NZ_JNCM01000031.1.
DR   AlphaFoldDB; P94407; -.
DR   SMR; P94407; -.
DR   STRING; 224308.BSU03660; -.
DR   ESTHER; bacsu-yclE; AlphaBeta_hydrolase.
DR   PaxDb; P94407; -.
DR   PRIDE; P94407; -.
DR   EnsemblBacteria; CAB12160; CAB12160; BSU_03660.
DR   GeneID; 938288; -.
DR   KEGG; bsu:BSU03660; -.
DR   PATRIC; fig|224308.179.peg.386; -.
DR   eggNOG; COG0596; Bacteria.
DR   InParanoid; P94407; -.
DR   OMA; YVETHGN; -.
DR   PhylomeDB; P94407; -.
DR   BioCyc; BSUB:BSU03660-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   Pfam; PF00561; Abhydrolase_1; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..281
FT                   /note="AB hydrolase superfamily protein YclE"
FT                   /id="PRO_0000360628"
FT   DOMAIN          30..268
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        95
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        232
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        261
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        32
FT                   /note="V -> A (in Ref. 1; BAA08999)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        91
FT                   /note="I -> T (in Ref. 1; BAA08999)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   281 AA;  32756 MW;  C450A493E8C7AB78 CRC64;
     MERAGICHSD GFDLAYRIEG EGAPILVIGS AVYYPRLFSS DIKQKYQWVF VDHRGFAKPK
     RELRAEDSRL DAVLADIERM RTFLQLEDVT ILGHSGHAFM ALEYARTYPK QVRKVALFNT
     APDNSEERQR KSESFFMETA SLERKKRFEK DIENLPQDID KDPERRFVHM CIRAEAKSFY
     QERPHAAALW DGVFTNMPII DELWGNTFAR IDLLQRLADV RMPVYIGLGR YDYLVAPVSL
     WDAVDGLYPH VDKVIFEKSG HQPMLEEPEA FDQSFRKWLD Q