ID   YCNI_BACSU              Reviewed;         204 AA.
AC   P94431; Q797N4;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Uncharacterized protein YcnI;
DE   Flags: Precursor;
GN   Name=ycnI; OrderedLocusNames=BSU03940;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969502; DOI=10.1099/13500872-142-11-3047;
RA   Yamane K., Kumano M., Kurita K.;
RT   "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT   determination of the sequence of a 146 kb segment and identification of 113
RT   genes.";
RL   Microbiology 142:3047-3056(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   INDUCTION.
RC   STRAIN=ATCC 21332 / IAM 1213;
RX   PubMed=19168619; DOI=10.1128/jb.01616-08;
RA   Chillappagari S., Miethke M., Trip H., Kuipers O.P., Marahiel M.A.;
RT   "Copper acquisition is mediated by ycnJ and regulated by ycnK and csoR in
RT   Bacillus subtilis.";
RL   J. Bacteriol. 191:2362-2370(2009).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass type I
CC       membrane protein {ECO:0000305}.
CC   -!- INDUCTION: Significantly induced under copper-limiting conditions.
CC       {ECO:0000269|PubMed:19168619}.
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DR   EMBL; D50453; BAA09025.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12202.1; -; Genomic_DNA.
DR   PIR; E69764; E69764.
DR   RefSeq; NP_388276.1; NC_000964.3.
DR   RefSeq; WP_003246657.1; NZ_JNCM01000031.1.
DR   PDB; 7ME6; X-ray; 2.05 A; A/B=27-155.
DR   PDB; 7MEK; X-ray; 2.11 A; A/B=27-155.
DR   PDBsum; 7ME6; -.
DR   PDBsum; 7MEK; -.
DR   AlphaFoldDB; P94431; -.
DR   SMR; P94431; -.
DR   STRING; 224308.BSU03940; -.
DR   jPOST; P94431; -.
DR   PaxDb; P94431; -.
DR   PRIDE; P94431; -.
DR   EnsemblBacteria; CAB12202; CAB12202; BSU_03940.
DR   GeneID; 938266; -.
DR   KEGG; bsu:BSU03940; -.
DR   PATRIC; fig|224308.179.peg.417; -.
DR   eggNOG; COG4549; Bacteria.
DR   InParanoid; P94431; -.
DR   OMA; NKEVVRW; -.
DR   PhylomeDB; P94431; -.
DR   BioCyc; BSUB:BSU03940-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   CDD; cd08545; YcnI_like; 1.
DR   Gene3D; 2.60.40.2230; -; 1.
DR   InterPro; IPR012533; YcnI-like.
DR   InterPro; IPR038507; YcnI-like_sf.
DR   Pfam; PF07987; DUF1775; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell membrane; Membrane; Reference proteome; Signal;
KW   Transmembrane; Transmembrane helix.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..204
FT                   /note="Uncharacterized protein YcnI"
FT                   /id="PRO_0000360196"
FT   TOPO_DOM        27..178
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        179..199
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        200..204
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          146..176
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        146..160
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   STRAND          29..31
FT                   /evidence="ECO:0007829|PDB:7ME6"
FT   STRAND          33..36
FT                   /evidence="ECO:0007829|PDB:7ME6"
FT   STRAND          40..48
FT                   /evidence="ECO:0007829|PDB:7ME6"
FT   STRAND          51..53
FT                   /evidence="ECO:0007829|PDB:7ME6"
FT   STRAND          55..61
FT                   /evidence="ECO:0007829|PDB:7ME6"
FT   STRAND          67..71
FT                   /evidence="ECO:0007829|PDB:7ME6"
FT   STRAND          77..83
FT                   /evidence="ECO:0007829|PDB:7ME6"
FT   STRAND          85..87
FT                   /evidence="ECO:0007829|PDB:7ME6"
FT   STRAND          89..98
FT                   /evidence="ECO:0007829|PDB:7ME6"
FT   STRAND          105..113
FT                   /evidence="ECO:0007829|PDB:7ME6"
FT   STRAND          119..123
FT                   /evidence="ECO:0007829|PDB:7ME6"
FT   STRAND          125..129
FT                   /evidence="ECO:0007829|PDB:7ME6"
FT   STRAND          134..137
FT                   /evidence="ECO:0007829|PDB:7ME6"
FT   STRAND          143..146
FT                   /evidence="ECO:0007829|PDB:7ME6"
FT   STRAND          148..153
FT                   /evidence="ECO:0007829|PDB:7ME6"
SQ   SEQUENCE   204 AA;  22113 MW;  61757652D7A09227 CRC64;
     MLKKIALTLC PAIVGSLLFF TAPASAHVSV KPAESAAGSW ETYTMKVPSE KNLPTTKVVL
     KMPKDVEFQQ YEPIPGWKVS TQKHDDKSVS VTWEATDGGI QEGQFQQFTF VAKNPDKAEE
     AAWDAYQYYK DGSIVEWTGD EDADTPHSIT NITSAKQVTD EHGATKTEDD SENSGSSALD
     ITAMVLSAAA IILSVAALVK KKRA