YCNK_BACSU
ID YCNK_BACSU Reviewed; 190 AA.
AC P94433; Q797N2;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=HTH-type transcriptional repressor YcnK;
GN Name=ycnK; OrderedLocusNames=BSU03960;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969502; DOI=10.1099/13500872-142-11-3047;
RA Yamane K., Kumano M., Kurita K.;
RT "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT determination of the sequence of a 146 kb segment and identification of 113
RT genes.";
RL Microbiology 142:3047-3056(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP FUNCTION AS YCNJ REGULATOR, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 21332 / IAM 1213;
RX PubMed=19168619; DOI=10.1128/jb.01616-08;
RA Chillappagari S., Miethke M., Trip H., Kuipers O.P., Marahiel M.A.;
RT "Copper acquisition is mediated by ycnJ and regulated by ycnK and csoR in
RT Bacillus subtilis.";
RL J. Bacteriol. 191:2362-2370(2009).
CC -!- FUNCTION: May act as a negative transcriptional regulator of ycnJ in
CC the presence of copper. May use copper as a corepressor.
CC {ECO:0000269|PubMed:19168619}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: Significantly induced under copper-limiting conditions.
CC {ECO:0000269|PubMed:19168619}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene show little or no
CC difference in growth under copper deprivation, whereas possessing
CC enhanced growth under copper excess conditions. Possesses a high
CC intracellular content of copper. {ECO:0000269|PubMed:19168619}.
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DR EMBL; D50453; BAA09027.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12204.1; -; Genomic_DNA.
DR PIR; G69764; G69764.
DR RefSeq; NP_388278.1; NC_000964.3.
DR RefSeq; WP_003246724.1; NZ_JNCM01000031.1.
DR AlphaFoldDB; P94433; -.
DR STRING; 224308.BSU03960; -.
DR PaxDb; P94433; -.
DR PRIDE; P94433; -.
DR EnsemblBacteria; CAB12204; CAB12204; BSU_03960.
DR GeneID; 938265; -.
DR KEGG; bsu:BSU03960; -.
DR PATRIC; fig|224308.179.peg.419; -.
DR eggNOG; COG1349; Bacteria.
DR InParanoid; P94433; -.
DR OMA; ACCEHCG; -.
DR PhylomeDB; P94433; -.
DR BioCyc; BSUB:BSU03960-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR001034; DeoR_HTH.
DR InterPro; IPR008719; N2O_reductase_NosL.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR41247; PTHR41247; 1.
DR Pfam; PF08220; HTH_DeoR; 1.
DR Pfam; PF05573; NosL; 1.
DR SMART; SM00420; HTH_DEOR; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS51000; HTH_DEOR_2; 1.
PE 1: Evidence at protein level;
KW Copper; Cytoplasm; DNA-binding; Metal-binding; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..190
FT /note="HTH-type transcriptional repressor YcnK"
FT /id="PRO_0000360705"
FT DOMAIN 3..58
FT /note="HTH deoR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00349"
FT DNA_BIND 20..39
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00349"
SQ SEQUENCE 190 AA; 21435 MW; F741317D6DF5C2B3 CRC64;
MLPINRQQHI LKWLKEEGSL RISDISARFG VSEMTVYRDV NQLVQSNQVI KTAGGITLPV
RTPQTDHMCS YCLKPVNQAH SVQLITVNQD IEQLCCAHCA FLRYADKTEE VSHLICRDFL
LQTTVSAGSA YFVVNAELNL HCCQPQAIPF ATLDHAERFQ KGFGGAVCTF DQALEDMLQD
RKKRCTCTKK
