ID   YCR2_BACTK              Reviewed;         252 AA.
AC   P21733;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   25-MAY-2022, entry version 40.
DE   RecName: Full=Uncharacterized 29.1 kDa protein in cryB1 5'region;
DE   AltName: Full=ORF2;
OS   Bacillus thuringiensis subsp. kurstaki.
OG   Plasmid unnamed.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=29339;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, OPERON, AND REPEAT.
RC   STRAIN=HD-1;
RX   PubMed=2914879; DOI=10.1128/jb.171.2.965-974.1989;
RA   Widner W.R., Whiteley H.R.;
RT   "Two highly related insecticidal crystal proteins of Bacillus thuringiensis
RT   subsp. kurstaki possess different host range specificities.";
RL   J. Bacteriol. 171:965-974(1989).
CC   -!- FUNCTION: A protein probably derived from this gene is found in
CC       cuboidal crystalline inclusions, but is not toxic even when coexpressed
CC       with upstream ORF1. The protein runs anomalously as a 50 kDa band in
CC       gels. {ECO:0000269|PubMed:2914879}.
CC   -!- INDUCTION: Transcribed starting in early sporulation and into later
CC       stages; not expressed during vegetative growth. Second gene in the
CC       orf1-orf2-cry2Aa (cryB1) operon. {ECO:0000305|PubMed:2914879}.
CC   -!- MISCELLANEOUS: Encoded on an unnamed 225 kb plasmid.
CC       {ECO:0000269|PubMed:2914879}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M23723; AAA83515.1; -; Genomic_DNA.
DR   PIR; B32053; B32053.
DR   RefSeq; WP_000922362.1; NZ_JABXXL010000001.1.
DR   AlphaFoldDB; P21733; -.
PE   2: Evidence at transcript level;
KW   Plasmid; Repeat.
FT   CHAIN           1..252
FT                   /note="Uncharacterized 29.1 kDa protein in cryB1 5'region"
FT                   /id="PRO_0000066178"
FT   REPEAT          68..82
FT                   /note="1"
FT                   /evidence="ECO:0000305|PubMed:2914879"
FT   REPEAT          83..97
FT                   /note="2"
FT                   /evidence="ECO:0000305|PubMed:2914879"
FT   REPEAT          98..112
FT                   /note="3"
FT                   /evidence="ECO:0000305|PubMed:2914879"
FT   REPEAT          113..127
FT                   /note="4"
FT                   /evidence="ECO:0000305|PubMed:2914879"
FT   REPEAT          128..142
FT                   /note="5"
FT                   /evidence="ECO:0000305|PubMed:2914879"
FT   REPEAT          143..157
FT                   /note="6"
FT                   /evidence="ECO:0000305|PubMed:2914879"
FT   REPEAT          158..172
FT                   /note="7"
FT                   /evidence="ECO:0000305|PubMed:2914879"
FT   REPEAT          173..187
FT                   /note="8"
FT                   /evidence="ECO:0000305|PubMed:2914879"
FT   REPEAT          188..202
FT                   /note="9"
FT                   /evidence="ECO:0000305|PubMed:2914879"
FT   REPEAT          203..217
FT                   /note="10"
FT                   /evidence="ECO:0000305|PubMed:2914879"
FT   REPEAT          218..230
FT                   /note="11; truncated"
FT                   /evidence="ECO:0000305|PubMed:2914879"
FT   REPEAT          231..239
FT                   /note="12; truncated"
FT                   /evidence="ECO:0000305|PubMed:2914879"
FT   REPEAT          240..246
FT                   /note="13; truncated"
FT                   /evidence="ECO:0000305|PubMed:2914879"
FT   REGION          68..246
FT                   /note="13 X 15 AA tandem repeats"
FT                   /evidence="ECO:0000305|PubMed:2914879"
SQ   SEQUENCE   252 AA;  29111 MW;  382F3BCB1EFFDEC9 CRC64;
     MLKYHFPNVC EDELINIYSY GDFKGQGKYI CLFKIENQSF LFWRNDKGNK IYTNLESISV
     EIINTNNTYN QSQNVCPQDL VDTYNQSQNV CPQDLVDTYN QSQNVCPQDL VDTYNQSQNV
     CPQDLVDTYN QSQNVCPQDL VDTYNQSQNV CPQDLVDTYN QSQNVYTQDL IDTYNQSQNV
     CPQDLVDTYN QSQNVCPQDL VDTYNQSQNV CPQDLVDTYN QSQNVCPQDL NVYTQDLIDT
     YNQSQNCDCG CK