ID   YCR2_PSEVU              Reviewed;         529 AA.
AC   Q01336;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Uncharacterized family 31 glucosidase ORF2;
DE            EC=3.2.1.-;
DE   Flags: Fragment;
OS   Pseudescherichia vulneris (Escherichia vulneris).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Pseudescherichia.
OX   NCBI_TaxID=566;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 39368 / Eho10;
RX   PubMed=7808389; DOI=10.1007/bf00302252;
RA   Hundle B., Alberti M., Nievelstein V., Beyer P., Kleinig H.,
RA   Armstrong G.A., Burke D.H., Hearst J.E.;
RT   "Functional assignment of Erwinia herbicola Eho10 carotenoid genes
RT   expressed in Escherichia coli.";
RL   Mol. Gen. Genet. 245:406-416(1994).
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 31 family. {ECO:0000305}.
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DR   EMBL; M87280; AAA64974.1; -; Genomic_DNA.
DR   PIR; S52976; S52976.
DR   AlphaFoldDB; Q01336; -.
DR   SMR; Q01336; -.
DR   CAZy; GH31; Glycoside Hydrolase Family 31.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR   InterPro; IPR000322; Glyco_hydro_31.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   Pfam; PF01055; Glyco_hydro_31; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   SUPFAM; SSF74650; SSF74650; 1.
PE   3: Inferred from homology;
KW   Glycosidase; Hydrolase.
FT   CHAIN           1..>529
FT                   /note="Uncharacterized family 31 glucosidase ORF2"
FT                   /id="PRO_0000185373"
FT   ACT_SITE        389
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        392
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        459
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   NON_TER         529
SQ   SEQUENCE   529 AA;  60812 MW;  24ECE92B09A01180 CRC64;
     MSELIEHANA IEWRFERQIL RLEPWGKNSL RVRATCAPEF TSALHALLTP ESCQAEILRD
     AETLTLRNGN ISAVLNLKGQ LAFYNQRGEL LLEEMWRQRS TVGIGASEKS QDKYVSALKL
     DGREFKPLPG GKYQLTVRFE ARQGEKLYGM GQYQQPWLDL KGCSLELAQR NSQASVPFVQ
     SSLGYGLLWN NPAIGEASFA KNHSQWTSRV TQEMDYWITA GDSVMEITRQ YAKATGTPPA
     APEFISGLWQ CKLRYRTQQE VLDVAREYHR RNLPLSVMVI DFFHWPNQGT WCFDPVDWPD
     PRAMVEELES MGIRLMVSVW PTVEARSPLF PQMKAKGWLV TSDRGVQVNL DFMGNTTFFD
     ATHPQARQFV WDTVKKNYFD YGIKLFWLDE AEPEYRAYDF DNYRYHAGPV LEVGNRYPRD
     FAQGFYDGLR AEGENEIVNL VRCSWAGSQR YGVLAWSGDV HSSFHSFRNQ LAAGLNMGLA
     GIPWWTTDIG GFQGGNIHDP AFHELLIRWF QWAVFCPVLR MHGYREPRI