CAZ_DROME
ID CAZ_DROME Reviewed; 399 AA.
AC Q27294; Q24445; Q8T3M2; Q9VXI4;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2004, sequence version 2.
DT 03-AUG-2022, entry version 176.
DE RecName: Full=RNA-binding protein cabeza;
DE AltName: Full=P19;
DE AltName: Full=Sarcoma-associated RNA-binding fly homolog;
GN Name=caz; Synonyms=SARFH; ORFNames=CG3606;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP STAGE, AND TISSUE SPECIFICITY.
RC STRAIN=Canton-S;
RX PubMed=7623847; DOI=10.1128/mcb.15.8.4562;
RA Immanuel D., Zinszner H., Ron D.;
RT "Association of SARFH (sarcoma-associated RNA-binding fly homolog) with
RT regions of chromatin transcribed by RNA polymerase II.";
RL Mol. Cell. Biol. 15:4562-4571(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=Canton-S; TISSUE=Pupae;
RX PubMed=7708500; DOI=10.1093/nar/23.5.835;
RA Stolow D.T., Haynes S.R.;
RT "Cabeza, a Drosophila gene encoding a novel RNA binding protein, shares
RT homology with EWS and TLS, two genes involved in human sarcoma formation.";
RL Nucleic Acids Res. 23:835-843(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 39-399.
RC STRAIN=Oregon-R; TISSUE=Pupae;
RA Haynes S.R.;
RL Submitted (APR-1988) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 80-91.
RC STRAIN=Berkeley; TISSUE=Ovary;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [8]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 212-261.
RC STRAIN=Oregon-R;
RX PubMed=3031652; DOI=10.1073/pnas.84.7.1819;
RA Haynes S.R., Rebbert M.L., Mozer B.A., Forquignon F., Dawid I.B.;
RT "Pen repeat sequences are GGN clusters and encode a glycine-rich domain in
RT a Drosophila cDNA homologous to the rat helix destabilizing protein.";
RL Proc. Natl. Acad. Sci. U.S.A. 84:1819-1823(1987).
CC -!- FUNCTION: May participate in a function common to the expression of
CC most genes transcribed by RNA polymerase II.
CC {ECO:0000269|PubMed:7623847}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:7623847}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. Enriched in the brain and central
CC nervous system during embryogenesis. Enriched in the adult head.
CC Embryos contain both isoforms A and B, whereas later in development
CC (heads and torsos) only isoform B is detected.
CC {ECO:0000269|PubMed:7623847, ECO:0000269|PubMed:7708500}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the developing embryo from the
CC earliest stages of cellularization and is subsequently found in many
CC cell types. {ECO:0000269|PubMed:7623847}.
CC -!- MISCELLANEOUS: 'Cabeza' means 'head' in Spanish.
CC -!- SIMILARITY: Belongs to the RRM TET family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM11116.1; Type=Erroneous translation; Note=Wrong choice of frame.; Evidence={ECO:0000305};
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DR EMBL; U13178; AAA86955.1; -; mRNA.
DR EMBL; L37083; AAC41563.1; -; mRNA.
DR EMBL; AE014298; AAN09389.1; -; Genomic_DNA.
DR EMBL; BT004875; AAO45231.1; -; mRNA.
DR EMBL; M15765; AAA70425.1; -; mRNA.
DR EMBL; AY094763; AAM11116.1; ALT_SEQ; mRNA.
DR PIR; S54729; S54729.
DR RefSeq; NP_523365.2; NM_078641.4.
DR AlphaFoldDB; Q27294; -.
DR SMR; Q27294; -.
DR BioGRID; 58928; 15.
DR IntAct; Q27294; 18.
DR STRING; 7227.FBpp0073996; -.
DR PaxDb; Q27294; -.
DR PRIDE; Q27294; -.
DR DNASU; 32587; -.
DR EnsemblMetazoa; FBtr0074217; FBpp0073996; FBgn0285954.
DR GeneID; 32587; -.
DR KEGG; dme:Dmel_CG3606; -.
DR CTD; 32587; -.
DR FlyBase; FBgn0285954; caz.
DR VEuPathDB; VectorBase:FBgn0285954; -.
DR eggNOG; KOG1995; Eukaryota.
DR GeneTree; ENSGT00940000169684; -.
DR InParanoid; Q27294; -.
DR OMA; DSYNKGP; -.
DR OrthoDB; 1539664at2759; -.
DR PhylomeDB; Q27294; -.
DR Reactome; R-DME-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-DME-6804756; Regulation of TP53 Activity through Phosphorylation.
DR Reactome; R-DME-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-DME-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-DME-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-DME-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-DME-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR SignaLink; Q27294; -.
DR BioGRID-ORCS; 32587; 0 hits in 3 CRISPR screens.
DR ChiTaRS; caz; fly.
DR GenomeRNAi; 32587; -.
DR PRO; PR:Q27294; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0285954; Expressed in eye disc (Drosophila) and 22 other tissues.
DR ExpressionAtlas; Q27294; baseline and differential.
DR Genevisible; Q27294; DM.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; HDA:FlyBase.
DR GO; GO:0005654; C:nucleoplasm; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0005669; C:transcription factor TFIID complex; ISS:FlyBase.
DR GO; GO:0003682; F:chromatin binding; IDA:FlyBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003729; F:mRNA binding; ISS:FlyBase.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0008344; P:adult locomotory behavior; IMP:FlyBase.
DR GO; GO:0048749; P:compound eye development; IMP:FlyBase.
DR GO; GO:0008345; P:larval locomotory behavior; IMP:FlyBase.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IC:FlyBase.
DR GO; GO:0045887; P:positive regulation of synaptic assembly at neuromuscular junction; IMP:FlyBase.
DR GO; GO:0051124; P:synaptic assembly at neuromuscular junction; IMP:FlyBase.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; ISS:FlyBase.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR034870; TET_fam.
DR InterPro; IPR001876; Znf_RanBP2.
DR InterPro; IPR036443; Znf_RanBP2_sf.
DR PANTHER; PTHR23238; PTHR23238; 1.
DR Pfam; PF00076; RRM_1; 1.
DR Pfam; PF00641; zf-RanBP; 1.
DR SMART; SM00360; RRM; 1.
DR SMART; SM00547; ZnF_RBZ; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR SUPFAM; SSF90209; SSF90209; 1.
DR PROSITE; PS50102; RRM; 1.
DR PROSITE; PS01358; ZF_RANBP2_1; 1.
DR PROSITE; PS50199; ZF_RANBP2_2; 1.
PE 2: Evidence at transcript level;
KW Metal-binding; Nucleus; Reference proteome; RNA-binding; Zinc; Zinc-finger.
FT CHAIN 1..399
FT /note="RNA-binding protein cabeza"
FT /id="PRO_0000081498"
FT DOMAIN 120..206
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT ZN_FING 275..304
FT /note="RanBP2-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00322"
FT REGION 1..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 209..277
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 300..399
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 16..38
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 39..41
FT /note="PNY -> LFI (in Ref. 6; AAA70425)"
FT /evidence="ECO:0000305"
FT CONFLICT 92
FT /note="H -> P (in Ref. 1; AAA86955, 2; AAC41563 and 6;
FT AAA70425)"
FT /evidence="ECO:0000305"
FT CONFLICT 109
FT /note="Missing (in Ref. 1; AAA86955, 2; AAC41563 and 6;
FT AAA70425)"
FT /evidence="ECO:0000305"
FT CONFLICT 253
FT /note="G -> GGNGGGG (in Ref. 1; AAA86955, 2; AAC41563 and
FT 6; AAA70425)"
FT /evidence="ECO:0000305"
FT CONFLICT 278
FT /note="D -> E (in Ref. 6; AAA70425)"
FT /evidence="ECO:0000305"
FT CONFLICT 384..393
FT /note="DGGPMRNDGG -> MVDQEKRWS (in Ref. 6; AAA70425)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 399 AA; 38839 MW; 3001FC96BDD6FDCA CRC64;
MERGGYGGGS GQGYNNFAVP PPNYQQMPNK TGNYNEPPPN YGKQGGGYDS GSGHRGSGGS
GNGGGGGGSW NDRGGNSYGN GGASKDSYNK GHGGYSGGGG GGGGGGGGGS GGNDMITQED
TIFVSGMDPS TTEQDIETHF GAIGIIKKDK RTMKPKIWLY KNKETGASKG EATVTYDDTN
AAQSAIEWFD GRDFNGNAIK VSLAQRQNNW NKGGGGGGGG GGRGGFGGRR GGGGGGGGGG
GGGGRFDRGG GGGGGRYDRG GGGGGGGGGG NVQPRDGDWK CNSCNNTNFA WRNECNRCKT
PKGDDEGSSG GGGGGGYGGG GGGGGYDRGN DRGSGGGGYH NRDRGGNSQG GGGGGGGGGG
YSRFNDNNGG GRGGRGGGGG NRRDGGPMRN DGGMRSRPY
