YCV1_YEAST
ID YCV1_YEAST Reviewed; 631 AA.
AC P25639; D6VR66; P25363; Q8NIL7;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 27-JUN-2003, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Uncharacterized membrane protein YCR061W;
DE Flags: Precursor;
GN OrderedLocusNames=YCR061W; ORFNames=YCR062W, YCR61W, YCR62W, YCR904;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=1574125; DOI=10.1038/357038a0;
RA Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M.,
RA Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G.,
RA Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A.,
RA Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M., Carcano C.,
RA Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M.,
RA Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C.,
RA Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F.,
RA Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C.,
RA Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E.,
RA Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P.,
RA Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J.,
RA Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P.,
RA Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M.,
RA Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P.,
RA Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G.,
RA Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E.,
RA Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F.,
RA Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L.,
RA Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J.,
RA Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M.,
RA Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A.,
RA Richterich P., Roberts A.B., Rodriguez F., Sanz E.,
RA Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J.,
RA Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I.,
RA Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M.,
RA Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M.,
RA Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D.,
RA Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K.,
RA Sgouros J.G.;
RT "The complete DNA sequence of yeast chromosome III.";
RL Nature 357:38-46(1992).
RN [2]
RP SEQUENCE REVISION.
RA Valles G., Volckaerts G.;
RL Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 512-631.
RX PubMed=1561837; DOI=10.1002/yea.320080209;
RA Benit P., Chanet R., Fabre F., Faye G., Fukuhara H., Sor F.;
RT "Sequence of the sup61-RAD18 region on chromosome III of Saccharomyces
RT cerevisiae.";
RL Yeast 8:147-153(1992).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 512-631.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [7]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-219, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-219, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
RN [10]
RP UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-250, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22106047; DOI=10.1002/pmic.201100166;
RA Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.;
RT "Sites of ubiquitin attachment in Saccharomyces cerevisiae.";
RL Proteomics 12:236-240(2012).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:14562095}; Multi-
CC pass membrane protein {ECO:0000269|PubMed:14562095}. Note=Localizes to
CC cytoplasmic punctate structures.
CC -!- SIMILARITY: To S.pombe SpBC3B8.06. {ECO:0000305}.
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DR EMBL; X59720; CAC42986.1; -; Genomic_DNA.
DR EMBL; AY693356; AAT93375.1; -; Genomic_DNA.
DR EMBL; BK006937; DAA07535.1; -; Genomic_DNA.
DR PIR; S19476; S19476.
DR PIR; S19477; S19477.
DR RefSeq; NP_009987.2; NM_001178772.1.
DR AlphaFoldDB; P25639; -.
DR SMR; P25639; -.
DR BioGRID; 31038; 163.
DR DIP; DIP-8245N; -.
DR IntAct; P25639; 22.
DR MINT; P25639; -.
DR STRING; 4932.YCR061W; -.
DR iPTMnet; P25639; -.
DR PaxDb; P25639; -.
DR PRIDE; P25639; -.
DR EnsemblFungi; YCR061W_mRNA; YCR061W; YCR061W.
DR GeneID; 850425; -.
DR KEGG; sce:YCR061W; -.
DR SGD; S000000657; YCR061W.
DR VEuPathDB; FungiDB:YCR061W; -.
DR eggNOG; ENOG502QW3E; Eukaryota.
DR GeneTree; ENSGT00940000176688; -.
DR HOGENOM; CLU_012543_1_0_1; -.
DR InParanoid; P25639; -.
DR OMA; VSISIMF; -.
DR BioCyc; YEAST:G3O-29366-MON; -.
DR PRO; PR:P25639; -.
DR Proteomes; UP000002311; Chromosome III.
DR RNAct; P25639; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008361; P:regulation of cell size; HMP:SGD.
DR InterPro; IPR018825; DUF2427.
DR InterPro; IPR018827; Uncharacterised_YCR061W_C.
DR Pfam; PF10348; DUF2427; 1.
DR Pfam; PF10355; Ytp1; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Isopeptide bond; Membrane; Phosphoprotein;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix;
KW Ubl conjugation.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..631
FT /note="Uncharacterized membrane protein YCR061W"
FT /id="PRO_0000014313"
FT TOPO_DOM 21..105
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 127..131
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 153..170
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 192..322
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 323..343
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 344..351
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 352..372
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 373..407
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 408..428
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 429..451
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 452..472
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 473..529
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 530..550
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 551..598
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 599..619
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 620..631
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 216..274
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 216..249
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 250..271
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 219
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956,
FT ECO:0007744|PubMed:19779198"
FT CARBOHYD 87
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 98
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CROSSLNK 250
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0007744|PubMed:22106047"
SQ SEQUENCE 631 AA; 70922 MW; 24B33124635FB508 CRC64;
MVRFVSILSL FGCAATLVTA HDDMDMDMDM DMDMDMNIDT TTSQSIDVSS TASIVPVPHE
PKHLHGLPIL QSPSLTPAER LYWENYNTTT YFTTQAGNRS ALRYHIITLL LVAFVLYPVS
LALSAARSRW YLPLLFVNLC ICISSVMALS VFKNTFPEED WYAHNIYGTT SVLLLVFMLV
HFFAAVLSVP VSLASKKEYR PVDTIPLNDL ESTPVMVNSA RGSPSPSSNR DTLFSLSSDT
TTATATNNNK RRRAEGEDEG DNTSNHDTLR DEDYDNDDDE IASIEAPPLL PQDIPVFRIL
FTNTKYQMLA AHLSCVANVV FHMLTYPLFM YIFVDLIIGF AVGNLLGKGI RIFNLLAHWI
KGGVFFTLGV VSLARYCGFA AKYGWAWNNI SFTSQLTQTR SSNLLFRFAP AGTFTMEFVE
SFLIFFYGST NIFLEHLAGN GGAWTAKDLQ HVSIAFMFIG TGLCGLLTEY KLNHWRFEHA
RKRPQTDVVA ATPGYSPNPF PAFTIFWTGI LMSQHAQSSQ FSTTIHTQWG YLLSYGSFFR
LLTFLILFLV PNTNSAASKP FTELITSFCL LCGGLVFMES TDQSIEAMEY RGFTPMFTFN
LSVGFVSLLM AWEMILFIWK DWLIKTRKTS L
