YD089_YEAST
ID YD089_YEAST Reviewed; 869 AA.
AC P38966; D6VS76; Q03192;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Uncharacterized protein YDR089W;
GN OrderedLocusNames=YDR089W; ORFNames=D4495, YD8554.22;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-241; SER-316; SER-327 AND
RP SER-668, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-238; SER-316; SER-324;
RP SER-327; SER-609; SER-612; SER-668 AND SER-760, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- MISCELLANEOUS: Present with 172 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; X82086; CAA57618.1; -; Genomic_DNA.
DR EMBL; Z46796; CAA86811.1; -; Genomic_DNA.
DR EMBL; Z74385; CAA98910.1; -; Genomic_DNA.
DR EMBL; Z50111; CAA90448.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11936.1; -; Genomic_DNA.
DR PIR; S49844; S49844.
DR RefSeq; NP_010374.1; NM_001180397.1.
DR AlphaFoldDB; P38966; -.
DR SMR; P38966; -.
DR BioGRID; 32146; 74.
DR STRING; 4932.YDR089W; -.
DR iPTMnet; P38966; -.
DR MaxQB; P38966; -.
DR PaxDb; P38966; -.
DR PRIDE; P38966; -.
DR EnsemblFungi; YDR089W_mRNA; YDR089W; YDR089W.
DR GeneID; 851663; -.
DR KEGG; sce:YDR089W; -.
DR SGD; S000002496; YDR089W.
DR VEuPathDB; FungiDB:YDR089W; -.
DR eggNOG; ENOG502QSRA; Eukaryota.
DR HOGENOM; CLU_016933_0_0_1; -.
DR InParanoid; P38966; -.
DR OMA; IRYWNEF; -.
DR BioCyc; YEAST:G3O-29694-MON; -.
DR PRO; PR:P38966; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; P38966; protein.
DR GO; GO:0000329; C:fungal-type vacuole membrane; HDA:SGD.
DR GO; GO:0071627; C:integral component of fungal-type vacuolar membrane; IDA:SGD.
DR GO; GO:0033254; C:vacuolar transporter chaperone complex; IPI:SGD.
DR GO; GO:0030643; P:cellular phosphate ion homeostasis; IMP:SGD.
DR GO; GO:0016237; P:lysosomal microautophagy; IBA:GO_Central.
DR GO; GO:0006799; P:polyphosphate biosynthetic process; IMP:SGD.
DR GO; GO:0006797; P:polyphosphate metabolic process; IBA:GO_Central.
DR GO; GO:0007034; P:vacuolar transport; IBA:GO_Central.
DR GO; GO:0042144; P:vacuole fusion, non-autophagic; IBA:GO_Central.
DR InterPro; IPR004331; SPX_dom.
DR PROSITE; PS51382; SPX; 1.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..869
FT /note="Uncharacterized protein YDR089W"
FT /id="PRO_0000202595"
FT DOMAIN 1..154
FT /note="SPX"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00714"
FT REGION 205..242
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 599..629
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 219..242
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 238
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 241
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 316
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956,
FT ECO:0007744|PubMed:19779198"
FT MOD_RES 324
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 327
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956,
FT ECO:0007744|PubMed:19779198"
FT MOD_RES 609
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 612
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 668
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956,
FT ECO:0007744|PubMed:19779198"
FT MOD_RES 760
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
SQ SEQUENCE 869 AA; 98712 MW; FA7E0BA84C32601E CRC64;
MKFEDRILNK SIPEWKFYNI NYEKLKVAIK KVTAYDYDNP NDSGMEKLLN QCSVAFDQEF
QNVNLFVSLK IKEISTRILS VESSIIDFSK GLNKTSRNRF NLRKLKIINA HVDDCNFELQ
LLSRFLIIQR IALRKLFKKL LNEFPQDSEN PLTASEYVTS IRNSESLRNG HEGISFMKLD
LDPYLLEVSL IVDVLHDLEN KLEDATEPAV EQRSLNRSDE SAHTSSSPEA NNSSLPASPR
SIPLLSNKKT SKMIDSSLEF DTALIDKAEN LGRFLLSSED IEGLKFMLLN IGFRIIDDSI
ISTSKEILDT TDNINSAGNK SIRSAKSFND LQHTLSLSKQ KNILPSAVQS NEKYVSISIL
DTVGNEGSPL LLTDDNINQH PNMIVSSTAE DTCIVMCHVG GLRNHVVTND LLLRDVKNIL
SAMRSGNDTK NISALINSLD PSPISKIALE WIQSHRLKTI EPKLDFKRTR FISADNGDIY
LIALDESITI GNVSTLPFPI LEIKKLSRSS GLSQTAINED NKFKQLMKSV VTNEFQCSLI
PPDLTTWKIC LELVHSNELQ NDLFQLLLRD QYKLNSDDSL SPDEFFQLGK DRLEEEFDLT
GPINNSQGSV DSGRRVRIHK KSKQSDNETK KKPIRYWNEF DEQEEDNLDN AFYIDTNGSR
STTDNEESLL LRNSPPDYGF ILFSRNFINR TYDFCEKLRN LIRHDKKTSP DLFQNSKHPH
CSSTNYGSVA SFGSQSTSAS YDDVQRYLQY QQQDIEDSQS IYEYRHDEVV TFLYLSALLT
SCIMASVCLG IVLSLFRGQS NNEIDLEIQN ILIAIIIISL LVSLILICAC LLLLFSRFTL
APIWHYVGCF TMFFSVTGTV CYGMIEIFF
