YD306_YEAST
ID YD306_YEAST Reviewed; 478 AA.
AC Q06640; D6VST5;
DT 17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=F-box protein YDR306C;
GN OrderedLocusNames=YDR306C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP INTERACTION WITH SKP1, RECONSTITUTION OF THE SCF(YDR306C) COMPLEX, AND
RP UBIQUITINATION.
RX PubMed=14747994; DOI=10.1002/prot.10620;
RA Kus B.M., Caldon C.E., Andorn-Broza R., Edwards A.M.;
RT "Functional interaction of 13 yeast SCF complexes with a set of yeast E2
RT enzymes in vitro.";
RL Proteins 54:455-467(2004).
CC -!- FUNCTION: Substrate recognition component of a SCF (SKP1-CUL1-F-box
CC protein) E3 ubiquitin-protein ligase complex which mediates the
CC ubiquitination and subsequent proteasomal degradation of target
CC proteins. Probably recognizes and binds to phosphorylated target
CC proteins (By similarity). {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Interacts with SKP1. Component of the probable SCF(YDR306C)
CC complex containing CDC53, SKP1, RBX1 and YDR306C.
CC {ECO:0000269|PubMed:14747994}.
CC -!- INTERACTION:
CC Q06640; P52286: SKP1; NbExp=3; IntAct=EBI-34097, EBI-4090;
CC -!- DOMAIN: The F-box domain contains an unusual insert of 18 residues
CC (131-148).
CC -!- PTM: Autoubiquitinated by the E3 ubiquitin ligase complex in
CC conjunction with the E2 enzyme CDC34. {ECO:0000269|PubMed:14747994}.
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DR EMBL; U28374; AAB64742.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA12145.1; -; Genomic_DNA.
DR PIR; S61192; S61192.
DR RefSeq; NP_010592.1; NM_001180614.1.
DR AlphaFoldDB; Q06640; -.
DR SMR; Q06640; -.
DR BioGRID; 32358; 62.
DR DIP; DIP-5370N; -.
DR IntAct; Q06640; 15.
DR MINT; Q06640; -.
DR STRING; 4932.YDR306C; -.
DR PaxDb; Q06640; -.
DR PRIDE; Q06640; -.
DR EnsemblFungi; YDR306C_mRNA; YDR306C; YDR306C.
DR GeneID; 851900; -.
DR KEGG; sce:YDR306C; -.
DR SGD; S000002714; YDR306C.
DR VEuPathDB; FungiDB:YDR306C; -.
DR eggNOG; ENOG502QSAP; Eukaryota.
DR HOGENOM; CLU_042679_1_0_1; -.
DR InParanoid; Q06640; -.
DR OMA; HACCLVS; -.
DR BioCyc; YEAST:G3O-29865-MON; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q06640; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q06640; protein.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; IDA:SGD.
DR GO; GO:0030674; F:protein-macromolecule adaptor activity; ISA:SGD.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; ISA:SGD.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR032675; LRR_dom_sf.
PE 1: Evidence at protein level;
KW Reference proteome; Ubl conjugation; Ubl conjugation pathway.
FT CHAIN 1..478
FT /note="F-box protein YDR306C"
FT /id="PRO_0000253805"
FT DOMAIN 112..173
FT /note="F-box"
FT REGION 1..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 67..101
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 478 AA; 54437 MW; F0F69FBE96FD0155 CRC64;
MANKSRPKKI KAPYRKYVAG EGFSSTRNDN KAKEFTITIP EDAELIETPQ GSYYYDETND
TIVKLTRLSN EKKDKKGRKQ SPSSSSTSSS KGEKNGKVIE SEEARMHSVS VKMVLPWEIQ
HRIIHYLDIP EKEEKLNKTA NGKKTTTGIN MNYLLVCRNW YAMCLPKLYY APALTSKNFN
GFVDTIIINK KKNLGHYVFE LNLSTILQSG RNSFVSKLLR RCCSNLTKFI APQTSFGYAP
LISLKSCHDL KFLDLGLVSE TVKLKELFSA IKNFTKLTHL SFPRSSIDCQ GFQDIQWPQN
LRYLKLSGGI TNEFVIDTKW PTTITTLEFS YCPQITELSI YSLLSQIGDN LKHLFFHYPM
PSLAENSLDH VFTYCANLIS LQLMVDYCSK WCFSEFMLSK LVEYDRPLKT LYLECSGSLG
LASKIHPDDL TIAILESRLP CLKNICVSPK LGWNMKSDEV ADLVVSLEDQ DGSLYLNY
