YDDG_ECOLI
ID YDDG_ECOLI Reviewed; 293 AA.
AC P46136; P76124; P77342;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 3.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Aromatic amino acid exporter YddG {ECO:0000305};
GN Name=yddG; OrderedLocusNames=b1473, JW1469;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-54.
RC STRAIN=K12;
RX PubMed=1834669; DOI=10.1016/s0021-9258(18)54583-x;
RA Berg B.L., Li J., Heider J., Stewart V.;
RT "Nitrate-inducible formate dehydrogenase in Escherichia coli K-12. I.
RT Nucleotide sequence of the fdnGHI operon and evidence that opal (UGA)
RT encodes selenocysteine.";
RL J. Biol. Chem. 266:22380-22385(1991).
RN [5]
RP IDENTIFICATION.
RX PubMed=7567469; DOI=10.1093/nar/23.17.3554;
RA Borodovsky M., McIninch J., Koonin E.V., Rudd K.E., Medigue C., Danchin A.;
RT "Detection of new genes in a bacterial genome using Markov models for three
RT gene classes.";
RL Nucleic Acids Res. 23:3554-3562(1995).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [7]
RP FUNCTION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=17784858; DOI=10.1111/j.1574-6968.2007.00894.x;
RA Doroshenko V., Airich L., Vitushkina M., Kolokolova A., Livshits V.,
RA Mashko S.;
RT "YddG from Escherichia coli promotes export of aromatic amino acids.";
RL FEMS Microbiol. Lett. 275:312-318(2007).
RN [8]
RP SUBCELLULAR LOCATION, AND TOPOLOGY.
RC STRAIN=K12;
RX PubMed=21042032; DOI=10.1159/000320699;
RA Airich L.G., Tsyrenzhapova I.S., Vorontsova O.V., Feofanov A.V.,
RA Doroshenko V.G., Mashko S.V.;
RT "Membrane topology analysis of the Escherichia coli aromatic amino acid
RT efflux protein YddG.";
RL J. Mol. Microbiol. Biotechnol. 19:189-197(2010).
RN [9]
RP FUNCTION.
RC STRAIN=K12;
RX PubMed=27281193; DOI=10.1038/nature17991;
RA Tsuchiya H., Doki S., Takemoto M., Ikuta T., Higuchi T., Fukui K.,
RA Usuda Y., Tabuchi E., Nagatoishi S., Tsumoto K., Nishizawa T., Ito K.,
RA Dohmae N., Ishitani R., Nureki O.;
RT "Structural basis for amino acid export by DMT superfamily transporter
RT YddG.";
RL Nature 534:417-420(2016).
CC -!- FUNCTION: Amino acid transporter with broad substrate specificity
CC (PubMed:17784858, PubMed:27281193). Can transport various amino acids,
CC including phenylalanine, tyrosine, tryptophan, L-threonine, L-
CC methionine, L-lysine, L-glutamate, L-valine and L-isoleucine
CC (PubMed:17784858, PubMed:27281193). Overexpression confers resistance
CC to phenylalanine and increases export of phenylalanine, tyrosine and
CC tryptophan (PubMed:17784858). {ECO:0000269|PubMed:17784858,
CC ECO:0000269|PubMed:27281193}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-phenylalanine(in) = L-phenylalanine(out);
CC Xref=Rhea:RHEA:27950, ChEBI:CHEBI:58095;
CC Evidence={ECO:0000305|PubMed:17784858};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-tyrosine(in) = L-tyrosine(out); Xref=Rhea:RHEA:68572,
CC ChEBI:CHEBI:58315; Evidence={ECO:0000305|PubMed:17784858};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-tryptophan(in) = L-tryptophan(out); Xref=Rhea:RHEA:70947,
CC ChEBI:CHEBI:57912; Evidence={ECO:0000305|PubMed:17784858};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-threonine(in) = L-threonine(out); Xref=Rhea:RHEA:35019,
CC ChEBI:CHEBI:57926; Evidence={ECO:0000305|PubMed:27281193};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-methionine(in) = L-methionine(out); Xref=Rhea:RHEA:70939,
CC ChEBI:CHEBI:57844; Evidence={ECO:0000305|PubMed:27281193};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-lysine(in) = L-lysine(out); Xref=Rhea:RHEA:70935,
CC ChEBI:CHEBI:32551; Evidence={ECO:0000305|PubMed:27281193};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-glutamate(out) = L-glutamate(in); Xref=Rhea:RHEA:66336,
CC ChEBI:CHEBI:29985; Evidence={ECO:0000305|PubMed:27281193};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-valine(in) = L-valine(out); Xref=Rhea:RHEA:29703,
CC ChEBI:CHEBI:57762; Evidence={ECO:0000305|PubMed:27281193};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-isoleucine(in) = L-isoleucine(out); Xref=Rhea:RHEA:70943,
CC ChEBI:CHEBI:58045; Evidence={ECO:0000305|PubMed:27281193};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:21042032}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:21042032}.
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. Aromatic amino acid/paraquat exporter (ArAA/P-E) (TC
CC 2.A.7.17) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA15122.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U00096; AAD13437.3; -; Genomic_DNA.
DR EMBL; AP009048; BAA15122.1; ALT_INIT; Genomic_DNA.
DR EMBL; M75029; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; D64900; D64900.
DR RefSeq; NP_415990.5; NC_000913.3.
DR RefSeq; WP_000198205.1; NZ_SSZK01000038.1.
DR AlphaFoldDB; P46136; -.
DR SMR; P46136; -.
DR BioGRID; 4260205; 15.
DR STRING; 511145.b1473; -.
DR TCDB; 2.A.7.17.2; the drug/metabolite transporter (dmt) superfamily.
DR PaxDb; P46136; -.
DR PRIDE; P46136; -.
DR EnsemblBacteria; AAD13437; AAD13437; b1473.
DR EnsemblBacteria; BAA15122; BAA15122; BAA15122.
DR GeneID; 945942; -.
DR KEGG; ecj:JW1469; -.
DR KEGG; eco:b1473; -.
DR PATRIC; fig|511145.12.peg.1539; -.
DR EchoBASE; EB2574; -.
DR eggNOG; COG0697; Bacteria.
DR HOGENOM; CLU_058959_1_1_6; -.
DR InParanoid; P46136; -.
DR OMA; VALWFHY; -.
DR PhylomeDB; P46136; -.
DR BioCyc; EcoCyc:EG12713-MON; -.
DR BioCyc; MetaCyc:EG12713-MON; -.
DR PRO; PR:P46136; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoCyc.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0015173; F:aromatic amino acid transmembrane transporter activity; IMP:EcoCyc.
DR GO; GO:0032973; P:amino acid export across plasma membrane; IDA:EcoCyc.
DR InterPro; IPR000620; EamA_dom.
DR Pfam; PF00892; EamA; 2.
PE 1: Evidence at protein level;
KW Amino-acid transport; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..293
FT /note="Aromatic amino acid exporter YddG"
FT /id="PRO_0000168943"
FT TOPO_DOM 1..6
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:21042032"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 28..33
FT /note="Periplasmic"
FT /evidence="ECO:0000269|PubMed:21042032"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 55..62
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:21042032"
FT TRANSMEM 63..83
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 84..92
FT /note="Periplasmic"
FT /evidence="ECO:0000269|PubMed:21042032"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 114..118
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:21042032"
FT TRANSMEM 119..139
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 140..155
FT /note="Periplasmic"
FT /evidence="ECO:0000269|PubMed:21042032"
FT TRANSMEM 156..176
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 177..182
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:21042032"
FT TRANSMEM 183..203
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 204..218
FT /note="Periplasmic"
FT /evidence="ECO:0000269|PubMed:21042032"
FT TRANSMEM 219..239
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 240..243
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:21042032"
FT TRANSMEM 244..264
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 265..267
FT /note="Periplasmic"
FT /evidence="ECO:0000269|PubMed:21042032"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 289..293
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:15919996,
FT ECO:0000269|PubMed:21042032"
FT DOMAIN 6..137
FT /note="EamA 1"
FT /evidence="ECO:0000255"
FT DOMAIN 158..285
FT /note="EamA 2"
FT /evidence="ECO:0000255"
SQ SEQUENCE 293 AA; 31539 MW; 45E02F367F3E562C CRC64;
MTRQKATLIG LIAIVLWSTM VGLIRGVSEG LGPVGGAAAI YSLSGLLLIF TVGFPRIRQI
PKGYLLAGSL LFVSYEICLA LSLGYAATHH QAIEVGMVNY LWPSLTILFA ILFNGQKTNW
LIVPGLLLAL VGVCWVLGGD NGLHYDEIIN NITTSPLSYF LAFIGAFIWA AYCTVTNKYA
RGFNGITVFV LLTGASLWVY YFLTPQPEMI FSTPVMIKLI SAAFTLGFAY AAWNVGILHG
NVTIMAVGSY FTPVLSSALA AVLLSAPLSF SFWQGALMVC GGSLLCWLAT RRG
