ID   YDEN_BACSU              Reviewed;         190 AA.
AC   P96671; Q797H7;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Putative hydrolase YdeN;
DE            EC=3.-.-.-;
GN   Name=ydeN; OrderedLocusNames=BSU05260;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.;
RT   "A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus
RT   subtilis genome.";
RL   Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS), AND PUTATIVE ACTIVE SITE.
RX   PubMed=15159570; DOI=10.1107/s0907444904007188;
RA   Janda I., Devedjiev Y., Cooper D., Chruszcz M., Derewenda U., Gabrys A.,
RA   Minor W., Joachimiak A., Derewenda Z.S.;
RT   "Harvesting the high-hanging fruit: the structure of the YdeN gene product
RT   from Bacillus subtilis at 1.8 Angstroms resolution.";
RL   Acta Crystallogr. D 60:1101-1107(2004).
CC   -!- SIMILARITY: Belongs to the RBBP9 family. {ECO:0000305}.
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DR   EMBL; AB001488; BAA19361.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12333.1; -; Genomic_DNA.
DR   PIR; H69778; H69778.
DR   RefSeq; NP_388407.1; NC_000964.3.
DR   RefSeq; WP_003234219.1; NZ_JNCM01000031.1.
DR   PDB; 1UXO; X-ray; 1.80 A; A=2-190.
DR   PDBsum; 1UXO; -.
DR   AlphaFoldDB; P96671; -.
DR   SMR; P96671; -.
DR   STRING; 224308.BSU05260; -.
DR   ESTHER; bacsu-YDEN; Hydrolase_RBBP9_YdeN.
DR   PaxDb; P96671; -.
DR   PRIDE; P96671; -.
DR   DNASU; 939908; -.
DR   EnsemblBacteria; CAB12333; CAB12333; BSU_05260.
DR   GeneID; 939908; -.
DR   KEGG; bsu:BSU05260; -.
DR   PATRIC; fig|224308.179.peg.561; -.
DR   eggNOG; COG3545; Bacteria.
DR   InParanoid; P96671; -.
DR   OMA; NHWFPWL; -.
DR   PhylomeDB; P96671; -.
DR   BioCyc; BSUB:BSU05260-MON; -.
DR   EvolutionaryTrace; P96671; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR010662; Hydrolase_RBBP9/YdeN.
DR   Pfam; PF06821; Ser_hydrolase; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Hydrolase; Reference proteome.
FT   CHAIN           1..190
FT                   /note="Putative hydrolase YdeN"
FT                   /id="PRO_0000360180"
FT   ACT_SITE        71
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        137
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        164
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255"
FT   STRAND          4..8
FT                   /evidence="ECO:0007829|PDB:1UXO"
FT   HELIX           20..29
FT                   /evidence="ECO:0007829|PDB:1UXO"
FT   STRAND          33..37
FT                   /evidence="ECO:0007829|PDB:1UXO"
FT   HELIX           47..55
FT                   /evidence="ECO:0007829|PDB:1UXO"
FT   HELIX           56..60
FT                   /evidence="ECO:0007829|PDB:1UXO"
FT   STRAND          65..70
FT                   /evidence="ECO:0007829|PDB:1UXO"
FT   HELIX           73..82
FT                   /evidence="ECO:0007829|PDB:1UXO"
FT   STRAND          90..96
FT                   /evidence="ECO:0007829|PDB:1UXO"
FT   HELIX           107..112
FT                   /evidence="ECO:0007829|PDB:1UXO"
FT   HELIX           119..125
FT                   /evidence="ECO:0007829|PDB:1UXO"
FT   STRAND          126..134
FT                   /evidence="ECO:0007829|PDB:1UXO"
FT   STRAND          138..140
FT                   /evidence="ECO:0007829|PDB:1UXO"
FT   HELIX           142..151
FT                   /evidence="ECO:0007829|PDB:1UXO"
FT   STRAND          155..159
FT                   /evidence="ECO:0007829|PDB:1UXO"
FT   HELIX           167..169
FT                   /evidence="ECO:0007829|PDB:1UXO"
FT   HELIX           175..186
FT                   /evidence="ECO:0007829|PDB:1UXO"
SQ   SEQUENCE   190 AA;  21722 MW;  B4D290FDDCD80362 CRC64;
     MTKQVYIIHG YRASSTNHWF PWLKKRLLAD GVQADILNMP NPLQPRLEDW LDTLSLYQHT
     LHENTYLVAH SLGCPAILRF LEHLQLRKQL GGIILVSGFA KSLPTLQMLD EFTQGSFDHQ
     KIIESAKHRA VIASKDDQIV PFSFSKDLAQ QIDAALYEVQ HGGHFLEDEG FTSLPIVYDV
     LTSYFSKETR