YDFJ_ECOLI
ID YDFJ_ECOLI Reviewed; 427 AA.
AC P77228; A0A385XJK1; P76884;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Putative transporter YdfJ;
GN Name=ydfJ; OrderedLocusNames=b1543, JW1536; ORFNames=b4600;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP SUBCELLULAR LOCATION, AND TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [5]
RP POSSIBLE FUNCTION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=21744086; DOI=10.1007/s00232-011-9386-2;
RA Tang G., Jiang B., Huang Y., Fu M., Wu L., Wang R.;
RT "Identification of a novel bacterial K(+) channel.";
RL J. Membr. Biol. 242:153-164(2011).
CC -!- FUNCTION: When overexpressed in human HEK-293 cells forms an inward
CC rectifying potassium channel. {ECO:0000269|PubMed:21744086}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein. Note=When
CC overexpressed using vectors that provide a promoter and ribosome
CC binding site (PubMed:15919996). {ECO:0000269|PubMed:15919996}.
CC -!- MISCELLANEOUS: YdfJ may constitute the C-terminal portion of an
CC ancestral gene disrupted by an insertion of the cryptic prophage
CC Qin/Kim. The N-terminal fragment may be ynfP. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Metabolite:H+
CC Symporter (MHS) family (TC 2.A.1.6) family. {ECO:0000305}.
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DR EMBL; U00096; AYC08214.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15248.1; -; Genomic_DNA.
DR PIR; B64909; B64909.
DR RefSeq; WP_000347482.1; NZ_CP011343.2.
DR AlphaFoldDB; P77228; -.
DR SMR; P77228; -.
DR BioGRID; 4260215; 134.
DR DIP; DIP-11699N; -.
DR IntAct; P77228; 1.
DR STRING; 316407.1742544; -.
DR TCDB; 2.A.1.6.9; the major facilitator superfamily (mfs).
DR PRIDE; P77228; -.
DR EnsemblBacteria; AYC08214; AYC08214; b1543.
DR EnsemblBacteria; BAA15248; BAA15248; BAA15248.
DR KEGG; ecj:JW1536; -.
DR PATRIC; fig|83333.103.peg.2375; -.
DR EchoBASE; EB3583; -.
DR eggNOG; COG0477; Bacteria.
DR HOGENOM; CLU_001265_39_5_6; -.
DR InParanoid; P77228; -.
DR OMA; EMFGSRN; -.
DR PhylomeDB; P77228; -.
DR BioCyc; EcoCyc:B1543-MON; -.
DR PRO; PR:P77228; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0005267; F:potassium channel activity; IDA:EcoCyc.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004736; MHS_symport.
DR Pfam; PF07690; MFS_1; 1.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00883; 2A0106; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..427
FT /note="Putative transporter YdfJ"
FT /id="PRO_0000050482"
FT TOPO_DOM 1..7
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 8..28
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 29..49
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 50..74
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 75..95
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 96..97
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 119..132
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 154..157
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..178
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 179..225
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..246
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 247..251
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 273..279
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 280..300
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 301..307
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 329..351
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 352..372
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 373
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..394
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 395..427
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255, ECO:0000269|PubMed:15919996"
SQ SEQUENCE 427 AA; 46267 MW; A15C00B8E2DCBD9B CRC64;
MDFQLYSLGA ALVFHEIFFP ESSTAMALIL AMGTYGAGYV ARIVGAFIFG KMGDRIGRKK
VLFITITMMG ICTTLIGVLP TYAQIGVFAP ILLVTLRIIQ GLGAGAEISG AGTMLAEYAP
KGKRGIISSF VAMGTNCGTL SATAIWAFMF FILSKEELLA WGWRIPFLAS VVVMVFAIWL
RMNLKESPVF EKVNDSNQPT AKPAPAGSMF QSKSFWLATG LRFGQAGNSG LIQTFLAGYL
VQTLLFNKAI PTDALMISSI LGFMTIPFLG WLSDKIGRRI PYIIMNTSAI VLAWPMLSII
VDKSYAPSTI MVALIVIHNC AVLGLFALEN ITMAEMFGCK NRFTRMAISK EIGGLIASGF
GPILAGIFCT MTESWYPIAI MIMAYSVIGL ISALKMPEVK DRDLSALEDA AEDQPRVVRA
AQPSRSL
