YDJA_ECOLI
ID YDJA_ECOLI Reviewed; 183 AA.
AC P0ACY1; P24250;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Putative NAD(P)H nitroreductase YdjA;
DE EC=1.-.-.-;
GN Name=ydjA; OrderedLocusNames=b1765, JW1754;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1650339; DOI=10.1128/jb.173.16.4983-4993.1991;
RA Sawers G., Heider J., Zehelein E., Boeck A.;
RT "Expression and operon structure of the sel genes of Escherichia coli and
RT identification of a third selenium-containing formate dehydrogenase
RT isoenzyme.";
RL J. Bacteriol. 173:4983-4993(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=B / BL21;
RX PubMed=10493123;
RX DOI=10.1002/(sici)1522-2683(19990801)20:11<2181::aid-elps2181>3.0.co;2-q;
RA Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B.;
RT "Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite
RT chromatography.";
RL Electrophoresis 20:2181-2195(1999).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH FMN, COFACTOR, AND
RP SUBUNIT.
RX PubMed=18241886; DOI=10.1016/j.jmb.2008.01.004;
RA Choi J.-W., Lee J., Nishi K., Kim Y.-S., Jung C.-H., Kim J.-S.;
RT "Crystal structure of a minimal nitroreductase, ydjA, from Escherichia coli
RT K12 with and without FMN cofactor.";
RL J. Mol. Biol. 377:258-267(2008).
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000269|PubMed:18241886};
CC Note=Binds 1 FMN per subunit. {ECO:0000269|PubMed:18241886};
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:18241886}.
CC -!- SIMILARITY: Belongs to the nitroreductase family. {ECO:0000305}.
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DR EMBL; M68961; AAA24245.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74835.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15556.1; -; Genomic_DNA.
DR PIR; A40360; A40360.
DR RefSeq; NP_416279.1; NC_000913.3.
DR RefSeq; WP_000339283.1; NZ_SSZK01000001.1.
DR PDB; 3BM1; X-ray; 2.00 A; A/B=1-183.
DR PDB; 3BM2; X-ray; 2.10 A; A/B=1-183.
DR PDBsum; 3BM1; -.
DR PDBsum; 3BM2; -.
DR AlphaFoldDB; P0ACY1; -.
DR SMR; P0ACY1; -.
DR BioGRID; 4259139; 163.
DR DIP; DIP-31841N; -.
DR IntAct; P0ACY1; 11.
DR STRING; 511145.b1765; -.
DR jPOST; P0ACY1; -.
DR PaxDb; P0ACY1; -.
DR PRIDE; P0ACY1; -.
DR EnsemblBacteria; AAC74835; AAC74835; b1765.
DR EnsemblBacteria; BAA15556; BAA15556; BAA15556.
DR GeneID; 66674340; -.
DR GeneID; 945964; -.
DR KEGG; ecj:JW1754; -.
DR KEGG; eco:b1765; -.
DR PATRIC; fig|1411691.4.peg.489; -.
DR EchoBASE; EB1124; -.
DR eggNOG; COG0778; Bacteria.
DR HOGENOM; CLU_070764_5_0_6; -.
DR InParanoid; P0ACY1; -.
DR OMA; IPEWEQV; -.
DR PhylomeDB; P0ACY1; -.
DR BioCyc; EcoCyc:EG11134-MON; -.
DR EvolutionaryTrace; P0ACY1; -.
DR PRO; PR:P0ACY1; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0010181; F:FMN binding; IDA:EcoCyc.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR CDD; cd02135; YdjA-like; 1.
DR Gene3D; 3.40.109.10; -; 1.
DR InterPro; IPR029479; Nitroreductase.
DR InterPro; IPR000415; Nitroreductase-like.
DR InterPro; IPR026021; YdjA-like.
DR Pfam; PF00881; Nitroreductase; 1.
DR PIRSF; PIRSF000232; YdjA; 1.
DR SUPFAM; SSF55469; SSF55469; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Flavoprotein; FMN; NAD; NADP; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..183
FT /note="Putative NAD(P)H nitroreductase YdjA"
FT /id="PRO_0000169002"
FT BINDING 10..12
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /ligand_note="ligand shared between dimeric partners"
FT /note="in other chain"
FT /evidence="ECO:0000269|PubMed:18241886"
FT BINDING 35
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /ligand_note="ligand shared between dimeric partners"
FT /evidence="ECO:0000269|PubMed:18241886"
FT BINDING 39
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /ligand_note="ligand shared between dimeric partners"
FT /evidence="ECO:0000269|PubMed:18241886"
FT BINDING 121..126
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000255"
FT BINDING 131..133
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /ligand_note="ligand shared between dimeric partners"
FT /note="in other chain"
FT /evidence="ECO:0000269|PubMed:18241886"
FT HELIX 3..9
FT /evidence="ECO:0007829|PDB:3BM1"
FT STRAND 16..18
FT /evidence="ECO:0007829|PDB:3BM1"
FT HELIX 23..33
FT /evidence="ECO:0007829|PDB:3BM1"
FT STRAND 46..51
FT /evidence="ECO:0007829|PDB:3BM1"
FT HELIX 53..69
FT /evidence="ECO:0007829|PDB:3BM1"
FT HELIX 73..81
FT /evidence="ECO:0007829|PDB:3BM1"
FT HELIX 82..85
FT /evidence="ECO:0007829|PDB:3BM1"
FT STRAND 86..94
FT /evidence="ECO:0007829|PDB:3BM1"
FT STRAND 100..102
FT /evidence="ECO:0007829|PDB:3BM1"
FT HELIX 104..123
FT /evidence="ECO:0007829|PDB:3BM1"
FT TURN 124..126
FT /evidence="ECO:0007829|PDB:3BM1"
FT STRAND 128..132
FT /evidence="ECO:0007829|PDB:3BM1"
FT HELIX 135..138
FT /evidence="ECO:0007829|PDB:3BM1"
FT HELIX 140..145
FT /evidence="ECO:0007829|PDB:3BM1"
FT STRAND 153..163
FT /evidence="ECO:0007829|PDB:3BM1"
FT HELIX 177..179
FT /evidence="ECO:0007829|PDB:3BM1"
FT STRAND 180..182
FT /evidence="ECO:0007829|PDB:3BM1"
SQ SEQUENCE 183 AA; 20059 MW; A69809EE378847CB CRC64;
MDALELLINR RSASRLAEPA PTGEQLQNIL RAGMRAPDHK SMQPWHFFVI EGEGRERFSA
VLEQGAIAAG SDDKAIDKAR NAPFRAPLII TVVAKCEENH KVPRWEQEMS AGCAVMAMQM
AAVAQGFGGI WRSGALTESP VVREAFGCRE QDKIVGFLYL GTPQLKASTS INVPDPTPFV
TYF
