YEA4_YEAST
ID YEA4_YEAST Reviewed; 342 AA.
AC P40004; D3DLP3;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=UDP-N-acetylglucosamine transporter YEA4;
GN Name=YEA4; OrderedLocusNames=YEL004W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=10788474; DOI=10.1074/jbc.275.18.13580;
RA Roy S.K., Chiba Y., Takeuchi M., Jigami Y.;
RT "Characterization of Yeast Yea4p, a uridine diphosphate-N-acetylglucosamine
RT transporter localized in the endoplasmic reticulum and required for chitin
RT synthesis.";
RL J. Biol. Chem. 275:13580-13587(2000).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: Sugar transporter that specifically mediates the transport of
CC UDP-N-acetylglucosamine (UDP-GlcNAc) and is required for cell wall
CC chitin synthesis. {ECO:0000269|PubMed:10788474}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum
CC {ECO:0000269|PubMed:10788474}. Endoplasmic reticulum membrane
CC {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the nucleotide-sugar transporter family. SLC35B
CC subfamily. {ECO:0000305}.
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DR EMBL; U18530; AAB64481.1; -; Genomic_DNA.
DR EMBL; BK006939; DAA07647.1; -; Genomic_DNA.
DR PIR; S50455; S50455.
DR RefSeq; NP_010912.1; NM_001178819.1.
DR AlphaFoldDB; P40004; -.
DR SMR; P40004; -.
DR BioGRID; 36727; 44.
DR DIP; DIP-8273N; -.
DR IntAct; P40004; 1.
DR MINT; P40004; -.
DR STRING; 4932.YEL004W; -.
DR TCDB; 2.A.7.10.5; the drug/metabolite transporter (dmt) superfamily.
DR PaxDb; P40004; -.
DR EnsemblFungi; YEL004W_mRNA; YEL004W; YEL004W.
DR GeneID; 856714; -.
DR KEGG; sce:YEL004W; -.
DR SGD; S000000730; YEA4.
DR VEuPathDB; FungiDB:YEL004W; -.
DR eggNOG; KOG1583; Eukaryota.
DR GeneTree; ENSGT00390000002915; -.
DR HOGENOM; CLU_033007_1_1_1; -.
DR InParanoid; P40004; -.
DR OMA; NYAFDFN; -.
DR BioCyc; YEAST:G3O-30133-MON; -.
DR Reactome; R-SCE-727802; Transport of nucleotide sugars.
DR PRO; PR:P40004; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P40004; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:SGD.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0030173; C:integral component of Golgi membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005462; F:UDP-N-acetylglucosamine transmembrane transporter activity; IMP:SGD.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0034221; P:fungal-type cell wall chitin biosynthetic process; IMP:SGD.
DR GO; GO:0055085; P:transmembrane transport; IMP:SGD.
DR GO; GO:0015786; P:UDP-glucose transmembrane transport; IMP:SGD.
DR GO; GO:1990569; P:UDP-N-acetylglucosamine transmembrane transport; IMP:SGD.
DR InterPro; IPR013657; UAA.
DR PANTHER; PTHR10778; PTHR10778; 1.
DR Pfam; PF08449; UAA; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Membrane; Reference proteome; Sugar transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..342
FT /note="UDP-N-acetylglucosamine transporter YEA4"
FT /id="PRO_0000213361"
FT TOPO_DOM 1..6
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 28..35
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 57..61
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 83..96
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 97..117
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 118..123
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 145..168
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..189
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 190..253
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 254..274
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 275..307
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 329..342
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 342 AA; 39266 MW; 39C68338A56E2D53 CRC64;
MWNSLKAFAL VFGGCCSNVI TFETLMSNET GSINNLITFC QFLFVTCQGL PEFLDVHQPF
PYFKPLKTPL HVYVITVVLF YISSTTNNNV FKYNISIPIH IVFRCFGTVI TMFTCWLLNG
RKYTKIQILS TLFLTIGAII ASLFKDADFR YQDLKLQAWK IGSDQSVDLT FIFGICILVL
SSFTSSLLSA YNERTYQKYG KHWKENIFYS HFLSLPLFLF SRKQLIHEYR VMRKSERILC
SNFGGKILVP REETLLLFNV LTQYFCVKGV NILASKTNAL TLSITLLVRK FISLLLSVRL
FDNNLSYTGY IGVYLVFFGA FIYSLGSIHP RQNDKGAIKK SK
