CB25_TETTH
ID CB25_TETTH Reviewed; 218 AA.
AC P09226;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 2.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=25 kDa calcium-binding protein;
DE AltName: Full=TCBP-25;
DE Contains:
DE RecName: Full=10 kDa calcium-binding protein;
DE AltName: Full=TCBP-10;
OS Tetrahymena thermophila.
OC Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC Oligohymenophorea; Hymenostomatida; Tetrahymenina; Tetrahymenidae;
OC Tetrahymena.
OX NCBI_TaxID=5911;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=B1868;
RX PubMed=2808424; DOI=10.1016/s0021-9258(19)47300-6;
RA Takemasa T., Ohnishi K., Kobayashi T., Takagi T., Konishi K., Watanabe Y.;
RT "Cloning and sequencing of the gene for Tetrahymena calcium-binding 25-kDa
RT protein (TCBP-25).";
RL J. Biol. Chem. 264:19293-19301(1989).
RN [2]
RP PROTEIN SEQUENCE OF 117-218.
RX PubMed=3391173; DOI=10.1111/j.1432-1033.1988.tb14137.x;
RA Kobayashi T., Takagi T., Konishi K., Ohnishi K., Watanabe Y.;
RT "Amino acid sequence of a calcium-binding protein (TCBP-10) from
RT Tetrahymena.";
RL Eur. J. Biochem. 174:579-584(1988).
RN [3]
RP CALCIUM-BINDING.
RX PubMed=6643461; DOI=10.1016/s0021-9258(17)44012-9;
RA Ohnishi K., Watanabe Y.;
RT "Purification and some properties of a new Ca2+-binding protein (TCBP-10)
RT present in tetrahymena cilium.";
RL J. Biol. Chem. 258:13978-13985(1983).
CC -!- FUNCTION: Expected to play a crucial role in calcium-dependent
CC regulation of ciliary movement.
CC -!- MISCELLANEOUS: The 10 kDa protein binds one calcium ion.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA30112.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; J05110; AAA30113.1; ALT_SEQ; mRNA.
DR EMBL; J05109; AAA30112.1; ALT_INIT; Genomic_DNA.
DR PIR; A34445; A34445.
DR PDB; 2NCO; NMR; -; A=117-218.
DR PDB; 2NCP; NMR; -; A=117-218.
DR PDBsum; 2NCO; -.
DR PDBsum; 2NCP; -.
DR AlphaFoldDB; P09226; -.
DR SMR; P09226; -.
DR OMA; MITEAYK; -.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0030030; P:cell projection organization; IEA:UniProtKB-KW.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 4.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 3.
DR PROSITE; PS50222; EF_HAND_2; 4.
PE 1: Evidence at protein level;
KW 3D-structure; Calcium; Cilium biogenesis/degradation;
KW Cleavage on pair of basic residues; Direct protein sequencing;
KW Metal-binding; Repeat.
FT CHAIN 1..218
FT /note="25 kDa calcium-binding protein"
FT /id="PRO_0000004140"
FT CHAIN 117..218
FT /note="10 kDa calcium-binding protein"
FT /id="PRO_0000004141"
FT DOMAIN 24..59
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 66..101
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 128..163
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 171..206
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 37
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000305"
FT BINDING 43
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000305"
FT BINDING 48
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000305"
FT BINDING 79
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 81
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 83
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 90
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 141
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 143
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 145
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 147
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 152
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 184
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 186
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 188
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 190
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 195
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT CONFLICT 124
FT /note="P -> N (in Ref. 1; AAA30113)"
FT /evidence="ECO:0000305"
FT HELIX 124..140
FT /evidence="ECO:0007829|PDB:2NCO"
FT STRAND 145..148
FT /evidence="ECO:0007829|PDB:2NCO"
FT HELIX 151..164
FT /evidence="ECO:0007829|PDB:2NCO"
FT HELIX 173..182
FT /evidence="ECO:0007829|PDB:2NCO"
FT STRAND 186..189
FT /evidence="ECO:0007829|PDB:2NCO"
FT HELIX 193..205
FT /evidence="ECO:0007829|PDB:2NCO"
FT TURN 206..208
FT /evidence="ECO:0007829|PDB:2NCO"
SQ SEQUENCE 218 AA; 24703 MW; E5B8DF06E9E08C8E CRC64;
MAQYSQTLRS SGFTSTVGLT DIEGAKTVAR RIFENYDKGR KGRIENTDCV PMITEAYKSF
NSFFAPSSDD IKAYHRVLDR NGDGIVTYQD IEELCIRYLT GTTVQRTIVT EEKVKKSSKP
KYNPEVEAKL DVARRLFKRY DKDGSGQLQD DEIAGLLKDT YAEMGMSNFT PTKEDVKIWL
QMADTNSDGS VSLEEYEDLI IKSLQKAGIR VEKQSLVF
