YEBT_ECOLI
ID YEBT_ECOLI Reviewed; 877 AA.
AC P76272; P97190; P97191;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Intermembrane transport protein YebT {ECO:0000305};
GN Name=yebT; OrderedLocusNames=b1834, JW1823;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION, AND SUBUNIT.
RC STRAIN=K12;
RX PubMed=28388411; DOI=10.1016/j.cell.2017.03.019;
RA Ekiert D.C., Bhabha G., Isom G.L., Greenan G., Ovchinnikov S.,
RA Henderson I.R., Cox J.S., Vale R.D.;
RT "Architectures of lipid transport systems for the bacterial outer
RT membrane.";
RL Cell 169:273-285(2017).
RN [5]
RP FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TOPOLOGY, AND INDUCTION.
RX PubMed=27795327; DOI=10.1128/jb.00606-16;
RA Nakayama T., Zhang-Akiyama Q.M.;
RT "pqiABC and yebST, putative mce operons of Escherichia coli, encode
RT transport pathways and contribute to membrane integrity.";
RL J. Bacteriol. 199:E00606-E00606(2017).
CC -!- FUNCTION: Component of a transport pathway that contributes to membrane
CC integrity (PubMed:27795327). May directly span the intermembrane space,
CC facilitating the transport of substrates across the periplasm
CC (Probable). {ECO:0000269|PubMed:27795327, ECO:0000305|PubMed:28388411}.
CC -!- SUBUNIT: Homohexamer (PubMed:28388411). May form a complex with YebS
CC (Probable). {ECO:0000269|PubMed:28388411, ECO:0000305|PubMed:27795327}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:27795327}; Single-pass membrane protein
CC {ECO:0000255}; Periplasmic side {ECO:0000269|PubMed:27795327}.
CC -!- INDUCTION: Induced by a defect in lipopolysaccharide molecules.
CC {ECO:0000269|PubMed:27795327}.
CC -!- SIMILARITY: Belongs to the PqiB family. {ECO:0000305}.
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DR EMBL; U00096; AAC74904.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA15647.1; -; Genomic_DNA.
DR PIR; B64945; B64945.
DR RefSeq; NP_416348.2; NC_000913.3.
DR RefSeq; WP_001326728.1; NZ_SSZK01000001.1.
DR PDB; 6KZ3; EM; 3.10 A; A/B/C/D/E/F=46-509.
DR PDB; 6KZ4; EM; 3.00 A; A/B/C/D/E/F=512-877.
DR PDB; 6V0C; EM; 3.46 A; A/B/C/D/E/F=46-877.
DR PDB; 6V0D; EM; 3.49 A; A/B/C/D/E/F=46-877.
DR PDB; 6V0E; EM; 3.06 A; A/B/C/D/E/F=46-877.
DR PDB; 6V0F; EM; 2.96 A; A/B/C/D/E/F=46-877.
DR PDB; 6V0G; EM; 3.03 A; A/B/C/D/E/F=46-877.
DR PDB; 6V0H; EM; 3.60 A; A/B/C/D/E/F=46-877.
DR PDB; 6V0I; EM; 3.43 A; A/B/C/D/E/F=46-877.
DR PDB; 6V0J; EM; 3.78 A; A/B/C/D/E/F=46-877.
DR PDB; 6VCI; X-ray; 2.15 A; A/C=159-383.
DR PDB; 7SEE; EM; 3.20 A; A/B/C/D/E/F=42-877.
DR PDB; 7SEF; EM; 3.55 A; A/B/C/D/E/F=42-877.
DR PDBsum; 6KZ3; -.
DR PDBsum; 6KZ4; -.
DR PDBsum; 6V0C; -.
DR PDBsum; 6V0D; -.
DR PDBsum; 6V0E; -.
DR PDBsum; 6V0F; -.
DR PDBsum; 6V0G; -.
DR PDBsum; 6V0H; -.
DR PDBsum; 6V0I; -.
DR PDBsum; 6V0J; -.
DR PDBsum; 6VCI; -.
DR PDBsum; 7SEE; -.
DR PDBsum; 7SEF; -.
DR AlphaFoldDB; P76272; -.
DR SMR; P76272; -.
DR BioGRID; 4259157; 10.
DR BioGRID; 850709; 1.
DR DIP; DIP-11815N; -.
DR IntAct; P76272; 6.
DR STRING; 511145.b1834; -.
DR TCDB; 9.A.69.1.2; the intermembrane phospholipid translocase (impl-t) family.
DR jPOST; P76272; -.
DR PaxDb; P76272; -.
DR PRIDE; P76272; -.
DR EnsemblBacteria; AAC74904; AAC74904; b1834.
DR EnsemblBacteria; BAA15647; BAA15647; BAA15647.
DR GeneID; 946352; -.
DR KEGG; ecj:JW1823; -.
DR KEGG; eco:b1834; -.
DR PATRIC; fig|511145.12.peg.1912; -.
DR EchoBASE; EB3776; -.
DR eggNOG; COG3008; Bacteria.
DR HOGENOM; CLU_015836_0_0_6; -.
DR InParanoid; P76272; -.
DR OMA; RFWNISG; -.
DR PhylomeDB; P76272; -.
DR BioCyc; EcoCyc:G7007-MON; -.
DR PRO; PR:P76272; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoCyc.
DR GO; GO:0030288; C:outer membrane-bounded periplasmic space; IDA:EcoCyc.
DR GO; GO:0042802; F:identical protein binding; IDA:EcoCyc.
DR GO; GO:0120009; P:intermembrane lipid transfer; IDA:EcoCyc.
DR InterPro; IPR003399; Mce/MlaD.
DR Pfam; PF02470; MlaD; 6.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Repeat; Transmembrane; Transmembrane helix.
FT CHAIN 1..877
FT /note="Intermembrane transport protein YebT"
FT /id="PRO_0000169055"
FT TOPO_DOM 1..19
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:27795327"
FT TRANSMEM 20..40
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 41..877
FT /note="Periplasmic"
FT /evidence="ECO:0000269|PubMed:27795327"
FT REGION 46..137
FT /note="MCE/MlaD 1"
FT /evidence="ECO:0000305"
FT REGION 163..220
FT /note="MCE/MlaD 2"
FT /evidence="ECO:0000305"
FT REGION 280..351
FT /note="MCE/MlaD 3"
FT /evidence="ECO:0000305"
FT REGION 394..455
FT /note="MCE/MlaD 4"
FT /evidence="ECO:0000305"
FT REGION 636..724
FT /note="MCE/MlaD 5"
FT /evidence="ECO:0000305"
FT REGION 747..810
FT /note="MCE/MlaD 6"
FT /evidence="ECO:0000305"
FT STRAND 49..54
FT /evidence="ECO:0007829|PDB:6V0G"
FT STRAND 61..63
FT /evidence="ECO:0007829|PDB:6V0G"
FT STRAND 65..68
FT /evidence="ECO:0007829|PDB:6V0G"
FT STRAND 71..82
FT /evidence="ECO:0007829|PDB:6V0G"
FT HELIX 84..86
FT /evidence="ECO:0007829|PDB:6V0G"
FT STRAND 87..94
FT /evidence="ECO:0007829|PDB:6V0G"
FT HELIX 96..101
FT /evidence="ECO:0007829|PDB:6V0G"
FT STRAND 107..111
FT /evidence="ECO:0007829|PDB:6V0G"
FT HELIX 117..121
FT /evidence="ECO:0007829|PDB:6V0G"
FT TURN 124..126
FT /evidence="ECO:0007829|PDB:6V0G"
FT STRAND 131..135
FT /evidence="ECO:0007829|PDB:6V0G"
FT STRAND 143..146
FT /evidence="ECO:0007829|PDB:6V0G"
FT STRAND 148..150
FT /evidence="ECO:0007829|PDB:6V0G"
FT STRAND 161..168
FT /evidence="ECO:0007829|PDB:6VCI"
FT STRAND 178..181
FT /evidence="ECO:0007829|PDB:6VCI"
FT STRAND 184..194
FT /evidence="ECO:0007829|PDB:6VCI"
FT STRAND 196..207
FT /evidence="ECO:0007829|PDB:6VCI"
FT HELIX 209..211
FT /evidence="ECO:0007829|PDB:6KZ3"
FT HELIX 212..214
FT /evidence="ECO:0007829|PDB:6VCI"
FT STRAND 221..227
FT /evidence="ECO:0007829|PDB:6VCI"
FT STRAND 237..240
FT /evidence="ECO:0007829|PDB:6V0F"
FT HELIX 244..246
FT /evidence="ECO:0007829|PDB:6VCI"
FT STRAND 247..253
FT /evidence="ECO:0007829|PDB:6VCI"
FT STRAND 257..259
FT /evidence="ECO:0007829|PDB:6KZ3"
FT STRAND 267..273
FT /evidence="ECO:0007829|PDB:6VCI"
FT HELIX 274..277
FT /evidence="ECO:0007829|PDB:6VCI"
FT STRAND 281..288
FT /evidence="ECO:0007829|PDB:6VCI"
FT TURN 295..297
FT /evidence="ECO:0007829|PDB:6VCI"
FT STRAND 298..302
FT /evidence="ECO:0007829|PDB:6VCI"
FT STRAND 305..315
FT /evidence="ECO:0007829|PDB:6VCI"
FT TURN 317..319
FT /evidence="ECO:0007829|PDB:6V0F"
FT STRAND 321..327
FT /evidence="ECO:0007829|PDB:6VCI"
FT HELIX 329..334
FT /evidence="ECO:0007829|PDB:6VCI"
FT STRAND 336..338
FT /evidence="ECO:0007829|PDB:6VCI"
FT STRAND 340..344
FT /evidence="ECO:0007829|PDB:6VCI"
FT STRAND 350..352
FT /evidence="ECO:0007829|PDB:6V0F"
FT HELIX 357..360
FT /evidence="ECO:0007829|PDB:6VCI"
FT STRAND 363..367
FT /evidence="ECO:0007829|PDB:6VCI"
FT STRAND 371..373
FT /evidence="ECO:0007829|PDB:6VCI"
FT STRAND 375..381
FT /evidence="ECO:0007829|PDB:6VCI"
FT HELIX 382..385
FT /evidence="ECO:0007829|PDB:6V0F"
FT TURN 386..388
FT /evidence="ECO:0007829|PDB:6V0F"
FT STRAND 389..391
FT /evidence="ECO:0007829|PDB:6V0F"
FT STRAND 393..401
FT /evidence="ECO:0007829|PDB:6V0F"
FT STRAND 410..413
FT /evidence="ECO:0007829|PDB:6V0F"
FT STRAND 416..426
FT /evidence="ECO:0007829|PDB:6V0F"
FT STRAND 431..437
FT /evidence="ECO:0007829|PDB:6V0F"
FT HELIX 439..444
FT /evidence="ECO:0007829|PDB:6V0F"
FT STRAND 449..453
FT /evidence="ECO:0007829|PDB:6V0F"
FT STRAND 458..460
FT /evidence="ECO:0007829|PDB:6V0I"
FT STRAND 467..470
FT /evidence="ECO:0007829|PDB:6V0I"
FT HELIX 473..478
FT /evidence="ECO:0007829|PDB:6V0F"
FT STRAND 481..485
FT /evidence="ECO:0007829|PDB:6V0F"
FT STRAND 493..496
FT /evidence="ECO:0007829|PDB:6V0F"
FT STRAND 498..500
FT /evidence="ECO:0007829|PDB:6V0F"
FT HELIX 501..505
FT /evidence="ECO:0007829|PDB:6V0F"
FT HELIX 506..508
FT /evidence="ECO:0007829|PDB:6KZ3"
FT STRAND 510..512
FT /evidence="ECO:0007829|PDB:6V0C"
FT STRAND 516..523
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 525..527
FT /evidence="ECO:0007829|PDB:6V0D"
FT STRAND 532..548
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 550..559
FT /evidence="ECO:0007829|PDB:6KZ4"
FT HELIX 561..566
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 569..575
FT /evidence="ECO:0007829|PDB:6KZ4"
FT HELIX 597..600
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 602..607
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 612..619
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 623..626
FT /evidence="ECO:0007829|PDB:6KZ4"
FT HELIX 627..631
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 634..642
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 652..655
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 658..663
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 667..669
FT /evidence="ECO:0007829|PDB:6KZ4"
FT TURN 670..673
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 674..681
FT /evidence="ECO:0007829|PDB:6KZ4"
FT TURN 683..688
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 689..691
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 695..699
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 702..704
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 707..709
FT /evidence="ECO:0007829|PDB:6KZ4"
FT HELIX 711..714
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 718..722
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 731..736
FT /evidence="ECO:0007829|PDB:6KZ4"
FT HELIX 742..744
FT /evidence="ECO:0007829|PDB:6V0E"
FT STRAND 745..747
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 749..755
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 765..768
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 771..780
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 783..786
FT /evidence="ECO:0007829|PDB:6V0E"
FT STRAND 787..794
FT /evidence="ECO:0007829|PDB:6KZ4"
FT HELIX 799..801
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 803..805
FT /evidence="ECO:0007829|PDB:6V0E"
FT STRAND 808..811
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 820..822
FT /evidence="ECO:0007829|PDB:6V0D"
FT HELIX 831..833
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 837..839
FT /evidence="ECO:0007829|PDB:6KZ4"
FT STRAND 857..860
FT /evidence="ECO:0007829|PDB:6KZ4"
FT HELIX 868..870
FT /evidence="ECO:0007829|PDB:6V0E"
SQ SEQUENCE 877 AA; 94970 MW; 3F4585F094D2D05A CRC64;
MSQETPASTT EAQIKNKRRI SPFWLLPFIA LMIASWLIWD SYQDRGNTVT IDFMSADGIV
PGRTPVRYQG VEVGTVQDIS LSDDLRKIEV KVSIKSDMKD ALREETQFWL VTPKASLAGV
SGLDALVGGN YIGMMPGKGK EQDHFVALDT QPKYRLDNGD LMIHLQAPDL GSLNSGSLVY
FRKIPVGKVY DYAINPNKQG VVIDVLIERR FTDLVKKGSR FWNVSGVDAN VSISGAKVKL
ESLAALVNGA IAFDSPEESK PAEAEDTFGL YEDLAHSQRG VIIKLELPSG AGLTADSTPL
MYQGLEVGQL TKLDLNPGGK VTGEMTVDPS VVTLLRENTR IELRNPKLSL SDANLSALLT
GKTFELVPGD GEPRKEFVVV PGEKALLHEP DVLTLTLTAP ESYGIDAGQP LILHGVQVGQ
VIDRKLTSKG VTFTVAIEPQ HRELVKGDSK FVVNSRVDVK VGLDGVEFLG ASASEWINGG
IRILPGDKGE MKASYPLYAN LEKALENSLS DLPTTTVSLS AETLPDVQAG SVVLYRKFEV
GEVITVRPRA NAFDIDLHIK PEYRNLLTSN SVFWAEGGAK VQLNGSGLTV QASPLSRALK
GAISFDNLSG ASASQRKGDK RILYASETAA RAVGGQITLH AFDAGKLAVG MPIRYLGIDI
GQIQTLDLIT ARNEVQAKAV LYPEYVQTFA RGGTRFSVVT PQISAAGVEH LDTILQPYIN
VEPGRGNPRR DFELQEATIT DSRYLDGLSI IVEAPEAGSL GIGTPVLFRG LEVGTVTGMT
LGTLSDRVMI AMRISKRYQH LVRNNSVFWL ASGYSLDFGL TGGVVKTGTF NQFIRGGIAF
ATPPGTPLAP KAQEGKHFLL QESEPKEWRE WGTALPK
