YEF5_YEAST
ID YEF5_YEAST Reviewed; 141 AA.
AC P32616;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Uncharacterized protein YEL045C;
GN OrderedLocusNames=YEL045C; ORFNames=SYGP-ORF33;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP INDUCTION.
RX PubMed=12453218; DOI=10.1046/j.1365-2958.2002.03246.x;
RA Serrano R., Ruiz A., Bernal D., Chambers J.R., Arino J.;
RT "The transcriptional response to alkaline pH in Saccharomyces cerevisiae:
RT evidence for calcium-mediated signalling.";
RL Mol. Microbiol. 46:1319-1333(2002).
RN [5]
RP DISRUPTION PHENOTYPE.
RX PubMed=14993228; DOI=10.1074/jbc.m313746200;
RA Serrano R., Bernal D., Simon E., Arino J.;
RT "Copper and iron are the limiting factors for growth of the yeast
RT Saccharomyces cerevisiae in an alkaline environment.";
RL J. Biol. Chem. 279:19698-19704(2004).
RN [6]
RP DISRUPTION PHENOTYPE.
RX PubMed=15060153; DOI=10.1128/mcb.24.8.3307-3323.2004;
RA Lawrence C.L., Botting C.H., Antrobus R., Coote P.J.;
RT "Evidence of a new role for the high-osmolarity glycerol mitogen-activated
RT protein kinase pathway in yeast: regulating adaptation to citric acid
RT stress.";
RL Mol. Cell. Biol. 24:3307-3323(2004).
RN [7]
RP DISRUPTION PHENOTYPE.
RX PubMed=15937126; DOI=10.1534/genetics.105.042812;
RA Sambade M., Alba M., Smardon A.M., West R.W., Kane P.M.;
RT "A genomic screen for yeast vacuolar membrane ATPase mutants.";
RL Genetics 170:1539-1551(2005).
CC -!- FUNCTION: Required for optimal growth under alkaline conditions.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Repressed after alkaline pH stress.
CC {ECO:0000269|PubMed:12453218}.
CC -!- DISRUPTION PHENOTYPE: Growth defect under alkaline conditions and
CC citric acid stress. {ECO:0000269|PubMed:14993228,
CC ECO:0000269|PubMed:15060153, ECO:0000269|PubMed:15937126}.
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DR EMBL; U18779; AAB64997.1; -; Genomic_DNA.
DR EMBL; AY558443; AAS56769.1; -; Genomic_DNA.
DR EMBL; BK006939; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; S30832; S30832.
DR AlphaFoldDB; P32616; -.
DR STRING; 4932.YEL045C; -.
DR PaxDb; P32616; -.
DR PRIDE; P32616; -.
DR EnsemblFungi; YEL045C_mRNA; YEL045C; YEL045C.
DR SGD; S000000771; YEL045C.
DR HOGENOM; CLU_1826406_0_0_1; -.
DR OMA; THAWPAM; -.
DR PRO; PR:P32616; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P32616; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
PE 2: Evidence at transcript level;
KW ATP-binding; Membrane; Nucleotide-binding; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..141
FT /note="Uncharacterized protein YEL045C"
FT /id="PRO_0000202606"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 67..87
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 15..22
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 141 AA; 16469 MW; F6604AC5343A5D5C CRC64;
MKCHAKRTLA FLATALPLSG KSRACTRTPQ SFASGFRAAA PFLFSRCFAL CITHCALFHL
PYSFTGFSFY FFVFFRLFLH WIYAFCICNS TYVYIYKQVL SLPVKSSMCP SRLSCSVWYR
VILTYIYVHC FRWLVRNHLL S
