ID   YEFM_ECOL6              Reviewed;          83 AA.
AC   P69347; P46147; P76371;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Antitoxin YefM;
GN   Name=yefM; OrderedLocusNames=c2545;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
RN   [2]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=23055930; DOI=10.1371/journal.ppat.1002954;
RA   Norton J.P., Mulvey M.A.;
RT   "Toxin-antitoxin systems are important for niche-specific colonization and
RT   stress resistance of uropathogenic Escherichia coli.";
RL   PLoS Pathog. 8:E1002954-E1002954(2012).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC       Antitoxin that counteracts the effect of the YoeB mRNA interferase.
CC       YefM binds to the promoter region of the yefM-yeoB operon to repress
CC       transcription, YeoB acts as a corepressor (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Forms a complex with YoeB which inhibits its toxin activity.
CC       {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the tomB-hha operon causes reduced
CC       infection of the mouse bladder but not of the kidney.
CC       {ECO:0000269|PubMed:23055930}.
CC   -!- SIMILARITY: Belongs to the phD/YefM antitoxin family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAN80999.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE014075; AAN80999.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_001259255.1; NC_004431.1.
DR   AlphaFoldDB; P69347; -.
DR   SMR; P69347; -.
DR   STRING; 199310.c2545; -.
DR   EnsemblBacteria; AAN80999; AAN80999; c2545.
DR   GeneID; 66674085; -.
DR   KEGG; ecc:c2545; -.
DR   eggNOG; COG2161; Bacteria.
DR   HOGENOM; CLU_155837_1_0_6; -.
DR   OMA; MRIVSFT; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR006442; Antitoxin_Phd/YefM.
DR   InterPro; IPR036165; YefM-like_sf.
DR   Pfam; PF02604; PhdYeFM_antitox; 1.
DR   SUPFAM; SSF143120; SSF143120; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Repressor; Toxin-antitoxin system; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..83
FT                   /note="Antitoxin YefM"
FT                   /id="PRO_0000213739"
SQ   SEQUENCE   83 AA;  9308 MW;  6D5C9A49DC8D8E8E CRC64;
     MRTISYSEAR QNLSATMMKA VEDHAPILIT RQNGEACVLM SLEEYNSLEE TAYLLRSPAN
     ARRLMDSIDS LKSGKGTEKD IIE