ID   YEGI_ECOLI              Reviewed;         648 AA.
AC   P76393; Q2MAX9;
DT   16-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Protein kinase YegI {ECO:0000305};
DE            EC=2.7.-.- {ECO:0000255|PROSITE-ProRule:PRU00159};
GN   Name=yegI; OrderedLocusNames=b2070, JW2055;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [3]
RP   FUNCTION, AND PHOSPHORYLATION.
RX   PubMed=29967116; DOI=10.1128/jb.00225-18;
RA   Rajagopalan K., Dworkin J.;
RT   "Identification and biochemical characterization of a novel PP2C-like
RT   Ser/Thr phosphatase in E. coli.";
RL   J. Bacteriol. 200:E00225-E00225(2018).
CC   -!- FUNCTION: Probable serine/threonine kinase.
CC       {ECO:0000269|PubMed:29967116}.
CC   -!- PTM: Autophosphorylated. Dephosphorylated by PphC.
CC       {ECO:0000269|PubMed:29967116}.
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DR   EMBL; U00096; AAC75131.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76577.1; -; Genomic_DNA.
DR   PIR; E64973; E64973.
DR   RefSeq; NP_416574.1; NC_000913.3.
DR   RefSeq; WP_000856081.1; NZ_LN832404.1.
DR   AlphaFoldDB; P76393; -.
DR   BioGRID; 4259694; 5.
DR   STRING; 511145.b2070; -.
DR   PaxDb; P76393; -.
DR   PRIDE; P76393; -.
DR   EnsemblBacteria; AAC75131; AAC75131; b2070.
DR   EnsemblBacteria; BAE76577; BAE76577; BAE76577.
DR   GeneID; 947159; -.
DR   KEGG; ecj:JW2055; -.
DR   KEGG; eco:b2070; -.
DR   PATRIC; fig|511145.12.peg.2147; -.
DR   EchoBASE; EB3805; -.
DR   eggNOG; COG4248; Bacteria.
DR   HOGENOM; CLU_022304_0_0_6; -.
DR   InParanoid; P76393; -.
DR   OMA; LADCPWC; -.
DR   BioCyc; EcoCyc:G7109-MON; -.
DR   PRO; PR:P76393; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004672; F:protein kinase activity; IEA:InterPro.
DR   GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR000719; Prot_kinase_dom.
DR   InterPro; IPR016960; YegI-like.
DR   PIRSF; PIRSF030823; UCP030823_PK_HhH; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
DR   PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Kinase; Nucleotide-binding; Phosphoprotein;
KW   Reference proteome; Transferase.
FT   CHAIN           1..648
FT                   /note="Protein kinase YegI"
FT                   /id="PRO_0000169123"
FT   DOMAIN          15..302
FT                   /note="Protein kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   ACT_SITE        143
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   BINDING         21..29
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   BINDING         41
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
SQ   SEQUENCE   648 AA;  71639 MW;  51142B51A6072975 CRC64;
     MKTNIKVFTS TGELTTLGRE LGKGGEGAVY DIEEFVDSVA KIYHTPPPAL KQDKLAFMAA
     TADAQLLNYV AWPQATLHGG RGGKVIGFMM PKVSGKEPIH MIYSPAHRRQ SYPHCAWDFL
     LYVARNIASS FATVHEHGHV VGDVNQNSFM VGRDSKVVLI DSDSFQINAN GTLHLCEVGV
     SHFTPPELQT LPSFVGFERT ENHDNFGLAL LIFHVLFGGR HPYSGVPLIS DAGNALETDI
     THFRYAYASD NQRRGLKPPP RSIPLSMLPS DVEAMFQQAF TESGVATGRP TAKAWVAALD
     SLRQQLKKCI VSAMHVYPAH LTDCPWCALD NQGVIYFIDL GEEVITTGGN FVLAKVWAMV
     MASVAPPALQ LPLPDHFQPT GRPLPLGLLR REYIILLEIA LSALSLLLCG LQAEPRYIIL
     VPVLAAIWII GSLTSKAYKA EVQQRREAFN RAKMDYDHLV RQIQQVGGLE GFIAKRTMLE
     KMKDEILGLP EEEKRALAAL HDTARERQKQ KFLEGFFIDV ASIPGVGPAR KAALRSFGIE
     TAADVTRRGV KQVKGFGDHL TQAVIDWKAS CERRFVFRPN EAITPADRQA VMAKMTAKRH
     RLESALTVGA TELQRFRLHA PARTMPLMEP LRQAAEKLAQ AQADLSRC