YEHB_ECOLI
ID YEHB_ECOLI Reviewed; 826 AA.
AC P33341;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Outer membrane usher protein YehB;
DE Flags: Precursor;
GN Name=yehB; OrderedLocusNames=b2109, JW2096;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / BHB2600;
RA Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K.,
RA Church G.M.;
RT "Automated multiplex sequencing of the E.coli genome.";
RL Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 307-826.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [5]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=20345943; DOI=10.1111/j.1462-2920.2010.02202.x;
RA Korea C.G., Badouraly R., Prevost M.C., Ghigo J.M., Beloin C.;
RT "Escherichia coli K-12 possesses multiple cryptic but functional chaperone-
RT usher fimbriae with distinct surface specificities.";
RL Environ. Microbiol. 12:1957-1977(2010).
CC -!- FUNCTION: Part of the yehABCD fimbrial operon. Could contribute to
CC adhesion to various surfaces in specific environmental niches. Probably
CC involved in the export and assembly of fimbrial subunits across the
CC outer membrane. {ECO:0000269|PubMed:20345943}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- INDUCTION: Expression is negatively regulated by H-NS and subjected to
CC cAMP receptor protein (CRP)-mediated catabolite repression.
CC {ECO:0000269|PubMed:20345943}.
CC -!- DISRUPTION PHENOTYPE: Deletion of the operon under classical laboratory
CC conditions does not result in any major effect on E.coli capacity to
CC form biofilms compared with the wild-type strain.
CC {ECO:0000269|PubMed:20345943}.
CC -!- MISCELLANEOUS: The operon is cryptic under classical laboratory
CC conditions, but is functional when constitutively expressed.
CC {ECO:0000305|PubMed:20345943}.
CC -!- SIMILARITY: Belongs to the fimbrial export usher family. {ECO:0000305}.
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DR EMBL; U00007; AAA60473.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75170.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15975.2; -; Genomic_DNA.
DR PIR; D64978; D64978.
DR RefSeq; NP_416612.1; NC_000913.3.
DR RefSeq; WP_000945454.1; NZ_LN832404.1.
DR AlphaFoldDB; P33341; -.
DR SMR; P33341; -.
DR BioGRID; 4260435; 265.
DR DIP; DIP-11896N; -.
DR IntAct; P33341; 2.
DR STRING; 511145.b2109; -.
DR TCDB; 1.B.11.3.11; the outer membrane fimbrial usher porin (fup) family.
DR PaxDb; P33341; -.
DR PRIDE; P33341; -.
DR EnsemblBacteria; AAC75170; AAC75170; b2109.
DR EnsemblBacteria; BAA15975; BAA15975; BAA15975.
DR GeneID; 946617; -.
DR KEGG; ecj:JW2096; -.
DR KEGG; eco:b2109; -.
DR PATRIC; fig|1411691.4.peg.138; -.
DR EchoBASE; EB1931; -.
DR eggNOG; COG3188; Bacteria.
DR HOGENOM; CLU_009120_1_1_6; -.
DR InParanoid; P33341; -.
DR OMA; YCTITFK; -.
DR PhylomeDB; P33341; -.
DR BioCyc; EcoCyc:EG11988-MON; -.
DR PRO; PR:P33341; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015473; F:fimbrial usher porin activity; IBA:GO_Central.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:EcoliWiki.
DR GO; GO:0009297; P:pilus assembly; IBA:GO_Central.
DR Gene3D; 2.60.40.2070; -; 1.
DR Gene3D; 2.60.40.2610; -; 1.
DR Gene3D; 3.10.20.410; -; 1.
DR InterPro; IPR000015; Fimb_usher.
DR InterPro; IPR018030; Fimbrial_membr_usher_CS.
DR InterPro; IPR042186; FimD_plug_dom.
DR InterPro; IPR025949; PapC-like_C.
DR InterPro; IPR043142; PapC-like_C_sf.
DR InterPro; IPR025885; PapC_N.
DR InterPro; IPR037224; PapC_N_sf.
DR PANTHER; PTHR30451; PTHR30451; 1.
DR Pfam; PF13953; PapC_C; 1.
DR Pfam; PF13954; PapC_N; 1.
DR Pfam; PF00577; Usher; 1.
DR SUPFAM; SSF141729; SSF141729; 1.
DR PROSITE; PS01151; FIMBRIAL_USHER; 1.
PE 2: Evidence at transcript level;
KW Cell outer membrane; Disulfide bond; Fimbrium biogenesis; Membrane;
KW Reference proteome; Signal; Transmembrane; Transmembrane beta strand;
KW Transport.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..826
FT /note="Outer membrane usher protein YehB"
FT /id="PRO_0000009331"
FT DISULFID 809..825
FT /evidence="ECO:0000255"
SQ SEQUENCE 826 AA; 92282 MW; 1FA541B1A21675CB CRC64;
MLRMTPLASA IVALLLGIEA YAAEETFDTH FMIGGMKDQQ VANIRLDDNQ PLPGQYDIDI
YVNKQWRGKY EIIVKDNPQE TCLSREVIKR LGINSDNFAS GKQCLTFEQL VQGGSYTWDI
GVFRLDFSVP QAWVEELESG YVPPENWERG INAFYTSYYL SQYYSDYKAS GNNKSTYVRF
NSGLNLLGWQ LHSDASFSKT NNNPGVWKSN TLYLERGFAQ LLGTLRVGDM YTSSDIFDSV
RFRGVRLFRD MQMLPNSKQN FTPRVQGIAQ SNALVTIEQN GFVVYQKEVP PGPFAITDLQ
LAGGGADLDV SVKEADGSVT TYLVPYAAVP NMLQPGVSKY DLAAGRSHIE GASKQSDFVQ
AGYQYGFNNL LTLYGGSMVA NNYYAFTLGA GWNTRIGAIS VDATKSHSKQ DNGDVFDGQS
YQIAYNKFVS QTSTRFGLAA WRYSSRDYRT FNDHVWANNK DNYRRDENDV YDIADYYQND
FGRKNSFSAN MSQSLPEGWG SVSLSTLWRD YWGRSGSSKD YQLSYSNNLR RISYTLAASQ
AYDENHHEEK RFNIFISIPF DWGDDVSTPR RQIYMSNSTT FDDQGFASNN TGLSGTVGSR
DQFNYGVNLS HQHQGNETTA GANLTWNAPV ATVNGSYSQS STYRQAGASV SGGIVAWSGG
VNLANRLSET FAVMNAPGIK DAYVNGQKYR TTNRNGVVIY DGMTPYRENH LMLDVSQSDS
EAELRGNRKI AAPYRGAVVL VNFDTDQRKP WFIKALRADG QSLTFGYEVN DIHGHNIGVV
GQGSQLFIRT NEVPPSVNVA IDKQQGLSCT ITFGKEIDES RNYICQ
