ID   YEHF_ECOLI              Reviewed;         274 AA.
AC   P33345; A0A385XJW5; P76427; P76428; P76429;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2017, sequence version 2.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Protein YehF;
GN   Name=yehF; Synonyms=dinO, molR {ECO:0000303|PubMed:2156810}, sosF;
GN   OrderedLocusNames=b2115; ORFNames=b2116/b2117, b4499;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / BHB2600;
RA   Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K.,
RA   Church G.M.;
RT   "Automated multiplex sequencing of the E.coli genome.";
RL   Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NOMENCLATURE.
RC   STRAIN=K12 / BW545;
RX   PubMed=2156810; DOI=10.1128/jb.172.4.2079-2087.1990;
RA   Lee J.H., Wendt J.C., Shanmugam K.T.;
RT   "Identification of a new gene, molR, essential for utilization of molybdate
RT   by Escherichia coli.";
RL   J. Bacteriol. 172:2079-2087(1990).
RN   [4]
RP   CHARACTERIZATION.
RA   Healy F., Grunden A., Ray R.M., Shanmugam K.T.;
RL   Unpublished observations (FEB-1994).
RN   [5]
RP   INDUCTION BY LEXA.
RC   STRAIN=K12 / RW118;
RX   PubMed=10760155; DOI=10.1046/j.1365-2958.2000.01826.x;
RA   Fernandez De Henestrosa A.R., Ogi T., Aoyagi S., Chafin D., Hayes J.J.,
RA   Ohmori H., Woodgate R.;
RT   "Identification of additional genes belonging to the LexA regulon in
RT   Escherichia coli.";
RL   Mol. Microbiol. 35:1560-1572(2000).
RN   [6]
RP   POTENTIAL INVOLVEMENT IN SELENATE REDUCTION.
RC   STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX   PubMed=12480890; DOI=10.1099/00221287-148-12-3865;
RA   Bebien M., Kirsch J., Mejean V., Vermeglio A.;
RT   "Involvement of a putative molybdenum enzyme in the reduction of selenate
RT   by Escherichia coli.";
RL   Microbiology 148:3865-3872(2002).
CC   -!- FUNCTION: Has been implicated in selenate reduction; a mini-Tn10
CC       insertion mutant in 'molR', (which was mapped to 47.3 centisomes i.e.
CC       this locus), is defective in the reduction of selenate.
CC       {ECO:0000269|PubMed:12480890}.
CC   -!- INDUCTION: Repressed by LexA, induced by DNA damage.
CC       {ECO:0000269|PubMed:10760155}.
CC   -!- CAUTION: Was originally thought to be involved in the regulation of
CC       molybdate metabolism, which gave rise to the name molR, however the
CC       locus in PubMed:2156810 was mapped at 65 centisomes (minutes) on the
CC       E.coli chromosome, while this locus maps to 47.3 centisomes
CC       (PubMed:2156810). This locus gives rise to a transcript that could
CC       encode the 'interrupted' N-terminal 274 amino acid gene product of
CC       yehF. The protein it encodes (shown here) is missing the C-terminus
CC       compared to orthologs. {ECO:0000269|PubMed:2156810, ECO:0000305}.
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DR   EMBL; U00007; AAA60477.1; -; Genomic_DNA.
DR   EMBL; U00096; AYC08227.1; -; Genomic_DNA.
DR   AlphaFoldDB; P33345; -.
DR   SMR; P33345; -.
DR   IntAct; P33345; 8.
DR   PRIDE; P33345; -.
DR   EnsemblBacteria; AYC08227; AYC08227; b2115.
DR   EchoBASE; EB1935; -.
DR   BioCyc; EcoCyc:EG11992-MON; -.
DR   Proteomes; UP000000625; Chromosome.
DR   InterPro; IPR036930; WGR_dom_sf.
DR   InterPro; IPR008893; WGR_domain.
DR   Pfam; PF05406; WGR; 1.
DR   SMART; SM00773; WGR; 1.
DR   SUPFAM; SSF142921; SSF142921; 1.
DR   PROSITE; PS51977; WGR; 1.
PE   1: Evidence at protein level;
KW   Reference proteome.
FT   CHAIN           1..274
FT                   /note="Protein YehF"
FT                   /id="PRO_0000096531"
FT   DOMAIN          2..78
FT                   /note="WGR"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01321"
SQ   SEQUENCE   274 AA;  30613 MW;  C6AF5B4064F68900 CRC64;
     MRHFIYQDEK SHKFRAVEQQ GNELHISWGK VGTKGQSQIK SFSDAAAAAK AELKLIAEKV
     KKGYVEQAKD NSLQPSQTVT GSLKVADLST IIQEQPSFVA ETRAPDKNTD AVLPWLAKDI
     AVVFPPEVVH TTLSHRRFPG VPVQQADKLP QLRRLACSVS QRDNKTATFD FSACSLEWQN
     TVAQAISQID GLKTTQLPSP VMAVLTALEM KCTRYKVRED VMDQIVQEGG LEYATDVIIH
     LQQIDIEWDY ANNVIIILPS GIAPSYLEQY SRFE