YEHF_ECOLI
ID YEHF_ECOLI Reviewed; 274 AA.
AC P33345; A0A385XJW5; P76427; P76428; P76429;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2017, sequence version 2.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Protein YehF;
GN Name=yehF; Synonyms=dinO, molR {ECO:0000303|PubMed:2156810}, sosF;
GN OrderedLocusNames=b2115; ORFNames=b2116/b2117, b4499;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / BHB2600;
RA Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K.,
RA Church G.M.;
RT "Automated multiplex sequencing of the E.coli genome.";
RL Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NOMENCLATURE.
RC STRAIN=K12 / BW545;
RX PubMed=2156810; DOI=10.1128/jb.172.4.2079-2087.1990;
RA Lee J.H., Wendt J.C., Shanmugam K.T.;
RT "Identification of a new gene, molR, essential for utilization of molybdate
RT by Escherichia coli.";
RL J. Bacteriol. 172:2079-2087(1990).
RN [4]
RP CHARACTERIZATION.
RA Healy F., Grunden A., Ray R.M., Shanmugam K.T.;
RL Unpublished observations (FEB-1994).
RN [5]
RP INDUCTION BY LEXA.
RC STRAIN=K12 / RW118;
RX PubMed=10760155; DOI=10.1046/j.1365-2958.2000.01826.x;
RA Fernandez De Henestrosa A.R., Ogi T., Aoyagi S., Chafin D., Hayes J.J.,
RA Ohmori H., Woodgate R.;
RT "Identification of additional genes belonging to the LexA regulon in
RT Escherichia coli.";
RL Mol. Microbiol. 35:1560-1572(2000).
RN [6]
RP POTENTIAL INVOLVEMENT IN SELENATE REDUCTION.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=12480890; DOI=10.1099/00221287-148-12-3865;
RA Bebien M., Kirsch J., Mejean V., Vermeglio A.;
RT "Involvement of a putative molybdenum enzyme in the reduction of selenate
RT by Escherichia coli.";
RL Microbiology 148:3865-3872(2002).
CC -!- FUNCTION: Has been implicated in selenate reduction; a mini-Tn10
CC insertion mutant in 'molR', (which was mapped to 47.3 centisomes i.e.
CC this locus), is defective in the reduction of selenate.
CC {ECO:0000269|PubMed:12480890}.
CC -!- INDUCTION: Repressed by LexA, induced by DNA damage.
CC {ECO:0000269|PubMed:10760155}.
CC -!- CAUTION: Was originally thought to be involved in the regulation of
CC molybdate metabolism, which gave rise to the name molR, however the
CC locus in PubMed:2156810 was mapped at 65 centisomes (minutes) on the
CC E.coli chromosome, while this locus maps to 47.3 centisomes
CC (PubMed:2156810). This locus gives rise to a transcript that could
CC encode the 'interrupted' N-terminal 274 amino acid gene product of
CC yehF. The protein it encodes (shown here) is missing the C-terminus
CC compared to orthologs. {ECO:0000269|PubMed:2156810, ECO:0000305}.
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DR EMBL; U00007; AAA60477.1; -; Genomic_DNA.
DR EMBL; U00096; AYC08227.1; -; Genomic_DNA.
DR AlphaFoldDB; P33345; -.
DR SMR; P33345; -.
DR IntAct; P33345; 8.
DR PRIDE; P33345; -.
DR EnsemblBacteria; AYC08227; AYC08227; b2115.
DR EchoBASE; EB1935; -.
DR BioCyc; EcoCyc:EG11992-MON; -.
DR Proteomes; UP000000625; Chromosome.
DR InterPro; IPR036930; WGR_dom_sf.
DR InterPro; IPR008893; WGR_domain.
DR Pfam; PF05406; WGR; 1.
DR SMART; SM00773; WGR; 1.
DR SUPFAM; SSF142921; SSF142921; 1.
DR PROSITE; PS51977; WGR; 1.
PE 1: Evidence at protein level;
KW Reference proteome.
FT CHAIN 1..274
FT /note="Protein YehF"
FT /id="PRO_0000096531"
FT DOMAIN 2..78
FT /note="WGR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01321"
SQ SEQUENCE 274 AA; 30613 MW; C6AF5B4064F68900 CRC64;
MRHFIYQDEK SHKFRAVEQQ GNELHISWGK VGTKGQSQIK SFSDAAAAAK AELKLIAEKV
KKGYVEQAKD NSLQPSQTVT GSLKVADLST IIQEQPSFVA ETRAPDKNTD AVLPWLAKDI
AVVFPPEVVH TTLSHRRFPG VPVQQADKLP QLRRLACSVS QRDNKTATFD FSACSLEWQN
TVAQAISQID GLKTTQLPSP VMAVLTALEM KCTRYKVRED VMDQIVQEGG LEYATDVIIH
LQQIDIEWDY ANNVIIILPS GIAPSYLEQY SRFE