YEHX_ECOLI
ID YEHX_ECOLI Reviewed; 308 AA.
AC P33360; Q2MAV0;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Glycine betaine uptake system ATP-binding protein YehX {ECO:0000305};
DE EC=7.4.2.- {ECO:0000305};
GN Name=yehX; OrderedLocusNames=b2129, JW2117;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / BHB2600;
RA Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K.,
RA Church G.M.;
RT "Automated multiplex sequencing of the E.coli genome.";
RL Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP INDUCTION.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=15251200; DOI=10.1016/j.femsle.2004.05.046;
RA Checroun C., Gutierrez C.;
RT "Sigma(s)-dependent regulation of yehZYXW, which encodes a putative
RT osmoprotectant ABC transporter of Escherichia coli.";
RL FEMS Microbiol. Lett. 236:221-226(2004).
RN [5]
RP FUNCTION IN GLYCINE BETAINE UPTAKE.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=26325238; DOI=10.1021/acs.biochem.5b00274;
RA Lang S., Cressatti M., Mendoza K.E., Coumoundouros C.N., Plater S.M.,
RA Culham D.E., Kimber M.S., Wood J.M.;
RT "YehZYXW of Escherichia coli is a low-affinity, non-osmoregulatory betaine-
RT specific ABC transporter.";
RL Biochemistry 54:5735-5747(2015).
CC -!- FUNCTION: Part of an ABC transporter complex involved in low-affinity
CC glycine betaine uptake. Probably responsible for energy coupling to the
CC transport system. {ECO:0000269|PubMed:26325238}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (YehX),
CC two transmembrane proteins (YehW and YehY) and a solute-binding protein
CC (YehZ). {ECO:0000305}.
CC -!- INDUCTION: Expression is sigma S-dependent. Induced by both osmotic
CC shock and entry into stationary phase. {ECO:0000269|PubMed:15251200}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. {ECO:0000305}.
CC -!- CAUTION: Was originally thought to be part of an osmoprotectant uptake
CC system (PubMed:15251200). However, it was shown later that the complex
CC does not mediate osmotic stress protection (PubMed:26325238).
CC {ECO:0000305|PubMed:15251200, ECO:0000305|PubMed:26325238}.
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DR EMBL; U00007; AAA60492.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75190.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76606.1; -; Genomic_DNA.
DR PIR; H64980; H64980.
DR RefSeq; NP_416633.1; NC_000913.3.
DR RefSeq; WP_000569315.1; NZ_LN832404.1.
DR AlphaFoldDB; P33360; -.
DR SMR; P33360; -.
DR BioGRID; 4260448; 33.
DR BioGRID; 851001; 1.
DR ComplexPortal; CPX-4449; Low affinity betaine ABC transporter complex.
DR DIP; DIP-11912N; -.
DR IntAct; P33360; 8.
DR STRING; 511145.b2129; -.
DR TCDB; 3.A.1.12.15; the atp-binding cassette (abc) superfamily.
DR jPOST; P33360; -.
DR PaxDb; P33360; -.
DR PRIDE; P33360; -.
DR EnsemblBacteria; AAC75190; AAC75190; b2129.
DR EnsemblBacteria; BAE76606; BAE76606; BAE76606.
DR GeneID; 946659; -.
DR KEGG; ecj:JW2117; -.
DR KEGG; eco:b2129; -.
DR PATRIC; fig|1411691.4.peg.115; -.
DR EchoBASE; EB1948; -.
DR eggNOG; COG1125; Bacteria.
DR HOGENOM; CLU_000604_1_5_6; -.
DR InParanoid; P33360; -.
DR OMA; DKICIMN; -.
DR PhylomeDB; P33360; -.
DR BioCyc; EcoCyc:YEHX-MON; -.
DR BioCyc; MetaCyc:YEHX-MON; -.
DR PRO; PR:P33360; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; IC:ComplexPortal.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR GO; GO:0015838; P:amino-acid betaine transport; IC:ComplexPortal.
DR GO; GO:0031460; P:glycine betaine transport; IDA:EcoCyc.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR046342; CBS_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF54631; SSF54631; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; ATP-binding; Nucleotide-binding; Reference proteome;
KW Translocase; Transport.
FT CHAIN 1..308
FT /note="Glycine betaine uptake system ATP-binding protein
FT YehX"
FT /id="PRO_0000093167"
FT DOMAIN 2..235
FT /note="ABC transporter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT BINDING 34..41
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
SQ SEQUENCE 308 AA; 34425 MW; 037C7C84E4EFB690 CRC64;
MIEFSHVSKL FGAQKAVNDL NLNFQEGSFS VLIGTSGSGK STTLKMINRL VEHDSGEIRF
AGEEIRSLPV LELRRRMGYA IQSIGLFPHW SVAQNIATVP QLQKWSRARI DDRIDELMAL
LGLESNLRER YPHQLSGGQQ QRVGVARALA ADPQVLLMDE PFGALDPVTR GALQQEMTRI
HRLLGRTIVL VTHDIDEALR LAEHLVLMDH GEVVQQGNPL TMLTRPANDF VRQFFGRSEL
GVRLLSLRSV ADYVRREERA DGEALAEEMT LRDALSLFVA RGCEVLPVVN MQGQPCGTLH
FQDLLVEA
