YEIL_ECOLI
ID YEIL_ECOLI Reviewed; 219 AA.
AC P0A9E9; P33023; Q2MAR6;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Regulatory protein YeiL;
GN Name=yeiL; Synonyms=nsr; OrderedLocusNames=b2163, JW2150;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / BHB2600;
RA Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K.,
RA Church G.M.;
RT "Automated multiplex sequencing of the E.coli genome.";
RL Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION, AND INDUCTION.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=11101674; DOI=10.1099/00221287-146-12-3157;
RA Anjum M.F., Green J., Guest J.R.;
RT "YeiL, the third member of the CRP-FNR family in Escherichia coli.";
RL Microbiology 146:3157-3170(2000).
CC -!- FUNCTION: Transcription regulator involved in mid-term, stationary-
CC phase viability under nitrogen starvation. Might control expression of
CC the salvage pathways or in some other way repress the recycling of
CC nucleobases to nucleic acids and enhance their use as general nitrogen
CC sources during nitrogen-limited growth. {ECO:0000269|PubMed:11101674}.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000305|PubMed:11101674};
CC Note=Binds 1 [4Fe-4S] cluster per subunit.
CC {ECO:0000305|PubMed:11101674};
CC -!- SUBUNIT: Homodimer. {ECO:0000305|PubMed:11101674}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|PubMed:11101674}.
CC -!- INDUCTION: In the stationary phase, aerobic expression is positively
CC autoregulated and significantly dependent on RpoS and Lrp. Its
CC transcription is directly or indirectly repressed by FNR.
CC {ECO:0000269|PubMed:11101674}.
CC -!- MISCELLANEOUS: Its activity might be modulated by a sensory iron-sulfur
CC cluster, by reversible dithiol-disulfide formation, or by both
CC mechanisms. It is not an oxygen-responsive regulator.
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DR EMBL; U00007; AAA60515.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75224.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76640.1; -; Genomic_DNA.
DR PIR; B64985; B64985.
DR RefSeq; NP_416668.3; NC_000913.3.
DR RefSeq; WP_001310375.1; NZ_JACEFS010000032.1.
DR AlphaFoldDB; P0A9E9; -.
DR SMR; P0A9E9; -.
DR BioGRID; 4260462; 27.
DR STRING; 511145.b2163; -.
DR PaxDb; P0A9E9; -.
DR PRIDE; P0A9E9; -.
DR EnsemblBacteria; AAC75224; AAC75224; b2163.
DR EnsemblBacteria; BAE76640; BAE76640; BAE76640.
DR GeneID; 946670; -.
DR KEGG; ecj:JW2150; -.
DR KEGG; eco:b2163; -.
DR PATRIC; fig|511145.12.peg.2248; -.
DR EchoBASE; EB1966; -.
DR eggNOG; COG0664; Bacteria.
DR HOGENOM; CLU_075053_11_1_6; -.
DR OMA; TSNVQVM; -.
DR PhylomeDB; P0A9E9; -.
DR BioCyc; EcoCyc:EG12031-MON; -.
DR PRO; PR:P0A9E9; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:transcription, DNA-templated; IEP:EcoCyc.
DR CDD; cd00038; CAP_ED; 1.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR018490; cNMP-bd-like.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR012318; HTH_CRP.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR Pfam; PF00027; cNMP_binding; 1.
DR SMART; SM00100; cNMP; 1.
DR SUPFAM; SSF51206; SSF51206; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
DR PROSITE; PS51063; HTH_CRP_2; 1.
PE 1: Evidence at protein level;
KW 4Fe-4S; Activator; Cytoplasm; DNA-binding; Iron; Iron-sulfur;
KW Metal-binding; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..219
FT /note="Regulatory protein YeiL"
FT /id="PRO_0000100182"
FT DOMAIN 136..199
FT /note="HTH crp-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00387"
FT DNA_BIND 158..181
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00387"
FT REGION 19..97
FT /note="Sensory domain"
FT /evidence="ECO:0000255"
FT REGION 111..131
FT /note="Dimer interface"
FT /evidence="ECO:0000255"
FT BINDING 68
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255"
FT BINDING 91
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255"
FT BINDING 93
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255"
FT BINDING 116
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255"
SQ SEQUENCE 219 AA; 25294 MW; 47720B07A9FD412D CRC64;
MSESAFKDCF LTDVSADTRL FHFLARDYIV QEGQQPSWLF YLTRGRARLY ATLANGRVSL
IDFFAAPCFI GEIELIDKDH EPRAVQAIEE CWCLALPMKH YRPLLLNDTL FLRKLCVTLS
HKNYRNIVSL TQNQSFPLVN RLAAFILLSQ EGDLYHEKHT QAAEYLGVSY RHLLYVLAQF
IHDGLLIKSK KGYLIKNRKQ LSGLALEMDP ENKFSGMMQ
