CB5D1_MOUSE
ID CB5D1_MOUSE Reviewed; 228 AA.
AC Q5NCY3; Q5NCY4;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Cytochrome b5 domain-containing protein 1;
GN Name=Cyb5d1; Synonyms=Gm740;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5NCY3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5NCY3-2; Sequence=VSP_029825, VSP_029826;
CC -!- SIMILARITY: Belongs to the cytochrome b5 family. {ECO:0000305}.
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DR EMBL; AL596125; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC099556; AAH99556.1; -; mRNA.
DR CCDS; CCDS24892.1; -. [Q5NCY3-1]
DR RefSeq; NP_001038990.1; NM_001045525.1. [Q5NCY3-1]
DR RefSeq; XP_011247375.1; XM_011249073.2. [Q5NCY3-1]
DR AlphaFoldDB; Q5NCY3; -.
DR SMR; Q5NCY3; -.
DR STRING; 10090.ENSMUSP00000059709; -.
DR iPTMnet; Q5NCY3; -.
DR PhosphoSitePlus; Q5NCY3; -.
DR PaxDb; Q5NCY3; -.
DR PRIDE; Q5NCY3; -.
DR ProteomicsDB; 279928; -. [Q5NCY3-1]
DR ProteomicsDB; 279929; -. [Q5NCY3-2]
DR Antibodypedia; 12269; 88 antibodies from 16 providers.
DR Ensembl; ENSMUST00000051620; ENSMUSP00000059709; ENSMUSG00000044795. [Q5NCY3-1]
DR Ensembl; ENSMUST00000108660; ENSMUSP00000104300; ENSMUSG00000044795. [Q5NCY3-2]
DR GeneID; 327951; -.
DR KEGG; mmu:327951; -.
DR UCSC; uc007jpz.1; mouse. [Q5NCY3-1]
DR CTD; 124637; -.
DR MGI; MGI:2685586; Cyb5d1.
DR VEuPathDB; HostDB:ENSMUSG00000044795; -.
DR eggNOG; KOG0537; Eukaryota.
DR GeneTree; ENSGT00440000037582; -.
DR HOGENOM; CLU_131019_0_0_1; -.
DR InParanoid; Q5NCY3; -.
DR OMA; DLTHFFH; -.
DR OrthoDB; 1534331at2759; -.
DR PhylomeDB; Q5NCY3; -.
DR TreeFam; TF324861; -.
DR BioGRID-ORCS; 327951; 2 hits in 73 CRISPR screens.
DR PRO; PR:Q5NCY3; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q5NCY3; protein.
DR Bgee; ENSMUSG00000044795; Expressed in ear vesicle and 235 other tissues.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 3.10.120.10; -; 1.
DR InterPro; IPR001199; Cyt_B5-like_heme/steroid-bd.
DR InterPro; IPR036400; Cyt_B5-like_heme/steroid_sf.
DR Pfam; PF00173; Cyt-b5; 1.
DR SMART; SM01117; Cyt-b5; 1.
DR SUPFAM; SSF55856; SSF55856; 1.
DR PROSITE; PS50255; CYTOCHROME_B5_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Heme; Iron; Metal-binding; Reference proteome.
FT CHAIN 1..228
FT /note="Cytochrome b5 domain-containing protein 1"
FT /id="PRO_0000312316"
FT DOMAIN 17..100
FT /note="Cytochrome b5 heme-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00279"
FT BINDING 83
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00279"
FT VAR_SEQ 153..175
FT /note="VGAQESMWEILHRYLPYNAHAAS -> LHMEIRWEEFEHGSNPGRKRDPG
FT (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_029825"
FT VAR_SEQ 176..228
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_029826"
SQ SEQUENCE 228 AA; 26719 MW; A0A4A6E86A5AC591 CRC64;
MPRRGLVAGP DLDNFQRRYF TPSEVAEHNQ LEDLWVSYLG FVYNLTPLVE EFKGDLLLKP
ILEVAGQDIS HWFDPQTRDI RKHIDPLTGC MRYRTPRGRF VHIPPPLPRS DWANDFGVPW
WKGANYQVGR LSARTRNIRI INTLATQEHT LQVGAQESMW EILHRYLPYN AHAASYTWKY
DGKNLNMDQT LEENGIRDEE EEFDYLNMDG KLHTPAILLY FNDDLTEL
