YELF2_DROME
ID YELF2_DROME Reviewed; 452 AA.
AC Q9VG08; Q95SK7;
DT 11-MAY-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 2.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=L-dopachrome tautomerase yellow-f2 {ECO:0000305};
DE EC=5.3.3.12 {ECO:0000269|PubMed:12164780};
DE Flags: Precursor;
GN Name=yellow-f2 {ECO:0000312|FlyBase:FBgn0038105};
GN ORFNames=CG8063 {ECO:0000312|FlyBase:FBgn0038105};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAL28276.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, CATALYTIC ACTIVITY,
RP BIOPHYSICOCHEMICAL PROPERTIES, PATHWAY, SUBCELLULAR LOCATION, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=12164780; DOI=10.1042/bj20020272;
RA Han Q., Fang J., Ding H., Johnson J.K., Christensen B.M., Li J.;
RT "Identification of Drosophila melanogaster yellow-f and yellow-f2 proteins
RT as dopachrome-conversion enzymes.";
RL Biochem. J. 368:333-340(2002).
CC -!- FUNCTION: Tautomerization of L-dopachrome with decarboxylation to give
CC 5,6-dihydroxyindole (DHI). Also catalyzes the tautomerization of the
CC methyl ester of L-dopachrome and dopamine chrome. May play a role in
CC melanization reactions during late pupal and adult stages.
CC {ECO:0000269|PubMed:12164780}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-dopachrome = 5,6-dihydroxyindole-2-carboxylate;
CC Xref=Rhea:RHEA:13041, ChEBI:CHEBI:16875, ChEBI:CHEBI:57509;
CC EC=5.3.3.12; Evidence={ECO:0000269|PubMed:12164780};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.11 mM for L-dopachrome {ECO:0000269|PubMed:12164780};
CC KM=0.31 mM for dopamine chrome {ECO:0000269|PubMed:12164780};
CC Vmax=123 umol/min/mg enzyme with L-dopachrome as substrate
CC {ECO:0000269|PubMed:12164780};
CC Vmax=52 umol/min/mg enzyme with dopamine chrome as substrate
CC {ECO:0000269|PubMed:12164780};
CC -!- PATHWAY: Pigment biosynthesis; melanin biosynthesis.
CC {ECO:0000269|PubMed:12164780}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:12164780}.
CC -!- DEVELOPMENTAL STAGE: Expressed at all stages with highest levels in
CC late-stage pupae and adults. {ECO:0000269|PubMed:12164780}.
CC -!- SIMILARITY: Belongs to the major royal jelly protein family.
CC {ECO:0000305}.
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DR EMBL; AE014297; AAF54885.2; -; Genomic_DNA.
DR EMBL; AY060728; AAL28276.1; -; mRNA.
DR RefSeq; NP_650247.1; NM_141990.3.
DR AlphaFoldDB; Q9VG08; -.
DR SMR; Q9VG08; -.
DR STRING; 7227.FBpp0082190; -.
DR GlyGen; Q9VG08; 2 sites.
DR PaxDb; Q9VG08; -.
DR PRIDE; Q9VG08; -.
DR DNASU; 41596; -.
DR EnsemblMetazoa; FBtr0082722; FBpp0082190; FBgn0038105.
DR GeneID; 41596; -.
DR KEGG; dme:Dmel_CG8063; -.
DR UCSC; CG8063-RA; d. melanogaster.
DR CTD; 41596; -.
DR FlyBase; FBgn0038105; yellow-f2.
DR VEuPathDB; VectorBase:FBgn0038105; -.
DR eggNOG; ENOG502SKJJ; Eukaryota.
DR GeneTree; ENSGT00530000064224; -.
DR HOGENOM; CLU_031076_2_0_1; -.
DR InParanoid; Q9VG08; -.
DR OMA; VMTNSMP; -.
DR OrthoDB; 940689at2759; -.
DR PhylomeDB; Q9VG08; -.
DR UniPathway; UPA00785; -.
DR BioGRID-ORCS; 41596; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 41596; -.
DR PRO; PR:Q9VG08; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0038105; Expressed in capitellum (Drosophila) and 13 other tissues.
DR GO; GO:0005576; C:extracellular region; IDA:FlyBase.
DR GO; GO:0004167; F:dopachrome isomerase activity; IDA:FlyBase.
DR GO; GO:0106417; F:dopaminechrome tautomerase activity; IDA:FlyBase.
DR GO; GO:0048066; P:developmental pigmentation; TAS:FlyBase.
DR GO; GO:0042435; P:indole-containing compound biosynthetic process; IDA:FlyBase.
DR GO; GO:0006583; P:melanin biosynthetic process from tyrosine; TAS:FlyBase.
DR Gene3D; 2.120.10.30; -; 1.
DR InterPro; IPR011042; 6-blade_b-propeller_TolB-like.
DR InterPro; IPR017996; Royal_jelly/protein_yellow.
DR PANTHER; PTHR10009; PTHR10009; 1.
DR Pfam; PF03022; MRJP; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Isomerase; Melanin biosynthesis; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..452
FT /note="L-dopachrome tautomerase yellow-f2"
FT /id="PRO_5006971588"
FT CARBOHYD 93
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 156
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 452 AA; 51029 MW; 261F3F68A56EA4F0 CRC64;
MLSPQFLILS LISGLQLLSL ADADPMIEVF RWKQMDFYNR GDGHLSSGGR KDRPSFSAPV
VFPGKYSRRK RKIMTSRDTP VVVSSRADSD DPNASYIPYN NVPMGATHFR GRLFVTMPRR
RVGIPSTLNY IDLAEDGSNR SPKLRAYPNF ALNQFNASAE NLVSVYRTSV DACQRLWFID
TGMLEYPNNR QQIRRPSIWV VDLATDQVLK RFDVPESIAE TGRGLASITV DVKAGQCGDA
YAYIPDLVYR RLYVYHLRND RIWSFEHNYF NFDPLSGDLS IGGQTFRWDD GIFSITLGAQ
KLDGSRDAYF HPMASTNEFV VSNRVLQQES NAARSDHGND FRVLGSRGPS TQSTMHAYDP
GTGVIFFDEI QRNGVGCWKT SKPISAENYG SVDSNAEDMI YPSDLSIDED GTIWVMSNSM
PIFIYSTLDT SIYNFRIWKQ KASLAKRGTV CE
