YELF_DROME
ID YELF_DROME Reviewed; 429 AA.
AC Q9VG09; Q86NV9;
DT 11-MAY-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=L-dopachrome tautomerase yellow-f {ECO:0000305};
DE EC=5.3.3.12 {ECO:0000269|PubMed:12164780};
DE Flags: Precursor;
GN Name=yellow-f {ECO:0000312|FlyBase:FBgn0041710};
GN ORFNames=CG18550 {ECO:0000312|FlyBase:FBgn0041710};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAO39616.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAO39616.1};
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K., Yu C., Lewis S.E., Rubin G.M., Celniker S.;
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, CATALYTIC ACTIVITY,
RP BIOPHYSICOCHEMICAL PROPERTIES, PATHWAY, SUBCELLULAR LOCATION, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=12164780; DOI=10.1042/bj20020272;
RA Han Q., Fang J., Ding H., Johnson J.K., Christensen B.M., Li J.;
RT "Identification of Drosophila melanogaster yellow-f and yellow-f2 proteins
RT as dopachrome-conversion enzymes.";
RL Biochem. J. 368:333-340(2002).
CC -!- FUNCTION: Tautomerization of L-dopachrome with decarboxylation to give
CC 5,6-dihydroxyindole (DHI). Also catalyzes the tautomerization of the
CC methyl ester of L-dopachrome and dopamine chrome. May play a role in
CC melanization reactions during late pupal and adult stages. May play a
CC role in melanization reactions during larval and early pupal stages.
CC {ECO:0000269|PubMed:12164780}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-dopachrome = 5,6-dihydroxyindole-2-carboxylate;
CC Xref=Rhea:RHEA:13041, ChEBI:CHEBI:16875, ChEBI:CHEBI:57509;
CC EC=5.3.3.12; Evidence={ECO:0000269|PubMed:12164780};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.33 mM for L-dopachrome {ECO:0000269|PubMed:12164780};
CC KM=0.29 mM for dopamine chrome {ECO:0000269|PubMed:12164780};
CC Vmax=137 umol/min/mg enzyme with L-dopachrome as substrate
CC {ECO:0000269|PubMed:12164780};
CC Vmax=32 umol/min/mg enzyme with dopamine chrome as substrate
CC {ECO:0000269|PubMed:12164780};
CC -!- PATHWAY: Pigment biosynthesis; melanin biosynthesis.
CC {ECO:0000269|PubMed:12164780}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:12164780}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=1;
CC IsoId=Q9VG09-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9VG09-2; Sequence=VSP_058327;
CC -!- DEVELOPMENTAL STAGE: Expressed in larvae and pupae, with highest levels
CC in later larval and early pupal stages. Very low expression in 1-day-
CC old adults. {ECO:0000269|PubMed:12164780}.
CC -!- MISCELLANEOUS: [Isoform 2]: Incomplete sequence. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the major royal jelly protein family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAO39616.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE014297; AAF54884.1; -; Genomic_DNA.
DR EMBL; BT003613; AAO39616.1; ALT_INIT; mRNA.
DR RefSeq; NP_524335.1; NM_079611.2. [Q9VG09-1]
DR AlphaFoldDB; Q9VG09; -.
DR SMR; Q9VG09; -.
DR STRING; 7227.FBpp0082191; -.
DR GlyGen; Q9VG09; 2 sites.
DR PaxDb; Q9VG09; -.
DR PRIDE; Q9VG09; -.
DR DNASU; 41595; -.
DR EnsemblMetazoa; FBtr0082723; FBpp0082191; FBgn0041710. [Q9VG09-1]
DR GeneID; 41595; -.
DR KEGG; dme:Dmel_CG18550; -.
DR UCSC; CG18550-RA; d. melanogaster. [Q9VG09-1]
DR CTD; 41595; -.
DR FlyBase; FBgn0041710; yellow-f.
DR VEuPathDB; VectorBase:FBgn0041710; -.
DR eggNOG; ENOG502SKJJ; Eukaryota.
DR GeneTree; ENSGT00530000064224; -.
DR InParanoid; Q9VG09; -.
DR PhylomeDB; Q9VG09; -.
DR UniPathway; UPA00785; -.
DR BioGRID-ORCS; 41595; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 41595; -.
DR PRO; PR:Q9VG09; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0041710; Expressed in embryonic/larval hemocyte (Drosophila) and 13 other tissues.
DR ExpressionAtlas; Q9VG09; baseline and differential.
DR Genevisible; Q9VG09; DM.
DR GO; GO:0005576; C:extracellular region; IDA:FlyBase.
DR GO; GO:0004167; F:dopachrome isomerase activity; IDA:FlyBase.
DR GO; GO:0106417; F:dopaminechrome tautomerase activity; IDA:FlyBase.
DR GO; GO:0048066; P:developmental pigmentation; TAS:FlyBase.
DR GO; GO:0042435; P:indole-containing compound biosynthetic process; IDA:FlyBase.
DR GO; GO:0006583; P:melanin biosynthetic process from tyrosine; IDA:FlyBase.
DR Gene3D; 2.120.10.30; -; 1.
DR InterPro; IPR011042; 6-blade_b-propeller_TolB-like.
DR InterPro; IPR017996; Royal_jelly/protein_yellow.
DR PANTHER; PTHR10009; PTHR10009; 1.
DR Pfam; PF03022; MRJP; 1.
PE 1: Evidence at protein level;
KW Alternative initiation; Glycoprotein; Isomerase; Melanin biosynthesis;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..429
FT /note="L-dopachrome tautomerase yellow-f"
FT /id="PRO_5004334647"
FT CARBOHYD 133
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 372
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT VAR_SEQ 1
FT /note="M -> SVTSGFSARKGERPEFYKM (in isoform 2)"
FT /id="VSP_058327"
SQ SEQUENCE 429 AA; 49108 MW; F51C270FC6DB7664 CRC64;
MLSLDVLLLC AISGFQLLIS ADGDPMIEVF KWKQLDFYNR GDGYKDLWNR ICIPDSHFYN
SRKCLGSSSS GSFIQYNNVP QGVTHFRGRL FVTVPRRQPG IPSTLNYIDL AKDGWSQSPH
LRAYPNLAVN QYNASEQNLV SVYRTSVDVC GRLWFVDTGM LEFPNNRQQI RHPSIWVIDL
ANDRLLKRFE IPQSIVEIGR GLASITIDVG ARRCNDAYAY IPDLVNRRLH VYHLRSDRIW
SFEHSFFNFD PLSDNLNIGG QTFRWDDGIF SATLGSYKPD GSRDVFFHPM ASTNEFVVSN
RVLQQEFNAA RSDHGDDFHL LGTRGPSTQS TMHKYDPRTG VIFFAEVQKS GVGCWKTSKP
FSTENHGSVY SNSSEMIYPS DLTIDEEGYI WVMSNSMPIF VYSKLDVEKY NFRIWRQSTL
LAKRGTVCE
