ID   YENA1_YERET             Reviewed;        1164 AA.
AC   B6A877;
DT   05-DEC-2018, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 1.
DT   25-MAY-2022, entry version 27.
DE   RecName: Full=Toxin subunit YenA1 {ECO:0000303|PubMed:21278295};
GN   Name=yenA1 {ECO:0000303|PubMed:21278295};
OS   Yersinia entomophaga.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=935293;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION BY MASS SPECTROMETRY,
RP   FUNCTION, ACTIVITY REGULATION, SUBUNIT, SUBCELLULAR LOCATION, AND
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC BAA-1678 / DSM 22339 / MH96;
RX   PubMed=21278295; DOI=10.1128/jb.01044-10;
RA   Hurst M.R., Jones S.A., Binglin T., Harper L.A., Jackson T.A., Glare T.R.;
RT   "The main virulence determinant of Yersinia entomophaga MH96 is a broad-
RT   host-range toxin complex active against insects.";
RL   J. Bacteriol. 193:1966-1980(2011).
RN   [2]
RP   ELECTRON MICROSCOPY (17.0 ANGSTROMS) OF YEN-TC:K9 COMPLEX, FUNCTION, AND
RP   SUBUNIT.
RC   STRAIN=ATCC BAA-1678 / DSM 22339 / MH96;
RX   PubMed=22158901; DOI=10.1073/pnas.1111155108;
RA   Landsberg M.J., Jones S.A., Rothnagel R., Busby J.N., Marshall S.D.,
RA   Simpson R.M., Lott J.S., Hankamer B., Hurst M.R.;
RT   "3D structure of the Yersinia entomophaga toxin complex and implications
RT   for insecticidal activity.";
RL   Proc. Natl. Acad. Sci. U.S.A. 108:20544-20549(2011).
CC   -!- FUNCTION: Part of an orally active toxin complex (TC) with strong
CC       insecticidal effects on larvae of the Coleoptera Costelytra zealandica,
CC       Acrossidius tasmania and Adoryphorus couloni and some Lepidoptera
CC       larvae (PubMed:21278295, PubMed:22158901). The TC has an endochitinase
CC       activity (PubMed:21278295) (Probable). {ECO:0000269|PubMed:21278295,
CC       ECO:0000269|PubMed:22158901, ECO:0000305|PubMed:22158901}.
CC   -!- ACTIVITY REGULATION: Toxin complex is secreted when grown at 25 degrees
CC       Celsius or less; at higher temperatures the proteins are present
CC       intracellularly but not secreted. {ECO:0000269|PubMed:21278295}.
CC   -!- SUBUNIT: Semipurified toxin complex consists of at least YenA1, YenA2,
CC       YenB, YenC1, YenC2, Chi1 and Chi2 (PubMed:21278295). The Yen-TC:K9
CC       subcomplex is about 26 nm tall and 22 nm in diameter with 5-fold
CC       symmetry and 5 copies of YenA1, YenA2, Chi1 and Chi2; the chitinase
CC       subunits may be solvent accessible on the exterior the complex
CC       (PubMed:22158901). The Yen-TC:K9 subcomplex has no insecticidal
CC       activity (PubMed:22158901). The native complex with additional YenB,
CC       YenC1 and YenC2 subunits is 16 nm taller and is insecticidal; the
CC       toxicity-conferring subunits are present at about 1 copy each
CC       (PubMed:22158901). {ECO:0000269|PubMed:21278295,
CC       ECO:0000269|PubMed:22158901}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:21278295}.
CC       Note=Secreted when grown at 25 degrees Celsius or less, but not when
CC       grown at 30 or 37 degrees Celsius. {ECO:0000269|PubMed:21278295}.
CC   -!- DISRUPTION PHENOTYPE: Cells with a disrupted yenA1-yenA2-chi2-yenB-
CC       yenC1-yenC2 locus are no longer pathogenic in C.zealandica larvae.
CC       {ECO:0000269|PubMed:21278295}.
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DR   EMBL; DQ400808; ABG33869.1; -; Genomic_DNA.
DR   RefSeq; WP_064513225.1; NZ_MWTM01000006.1.
DR   PDB; 6OGD; EM; 4.40 A; A/D/G/J/M=1-1164.
DR   PDBsum; 6OGD; -.
DR   AlphaFoldDB; B6A877; -.
DR   SMR; B6A877; -.
DR   DIP; DIP-60373N; -.
DR   IntAct; B6A877; 1.
DR   STRING; 935293.PL78_03745; -.
DR   PATRIC; fig|935293.3.peg.806; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR018003; Insecticidal_toxin/plasmid_vir.
DR   Pfam; PF03538; VRP1; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Secreted; Virulence.
FT   CHAIN           1..1164
FT                   /note="Toxin subunit YenA1"
FT                   /id="PRO_0000445771"
FT   REGION          106..131
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        110..131
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1164 AA;  129805 MW;  CA633800C1765EBC CRC64;
     MDKYNNYSNV IKNKSSISPL LAAAAKIEPE ITVLSSASKS NRSQYSQSLA DTLLGLGYRS
     IFDIAKVSRQ RFIKRHDESL LGNGAVIFDK AVSMANQVLQ KYRKNRLEKS NSPLVPQTSS
     STDASSESQT NKLPEYNQLF PEPWDNFCRP GAIEALDSPA SYLLDLYKFI QSVELDGSNQ
     ARKLETRRAD IPKLSLDNDA LYKEVTALSI VNDVLSGSAR EYIDQSGQAD KAVNQILGDT
     HFPFTLPYSL PTQQINKGLG ASNIELGTVI QRVDPQFSWN TTQEKYNQVL LAYTQLSSEQ
     IALLSLPDVF TQNFLTQTEL SAGYLSASTT EILAEKDLSR HGYIVKAADN IKGPTQLVEH
     SDASYDVIEL TCTNQAKETI TVKLRGENII TYQRTKARMV PFDNSSPFSR QLKLTFVAED
     NPSLGNLDKG PYFANMDIYA AEWVRENVSS ETMVSRPFLT MTYRIAIAKA GASLEELQPE
     ADAFFINNFG LSAEDSSQLV KLVAFGDQTG SKAEEIESLL SCGENLPIVS PNVIFANPIF
     GSYFNDEPFP APYHFGGVYI NAHQRNAMTI IRAEGGREIQ SLSNFRLERL NRFIRLQRWL
     DLPSHQLDLL LTSVMQADAD NSQQEITEPV LKSLGLFRHL NLQYKITPEI FSSWLYQLTP
     FAVSGEIAFF DRIFNREQLF DQPFILDGGS FTYLDAKGSD AKSVKQLCAG LNISAVTFQF
     IAPLVQSALG LEAGTLVRSF EVVSSLYRLV SIPQTFGLST EDGLILMNIL TDEMGYLAKQ
     PAFDDKQTQD KDFLSIILKM EALSAWLTKN NLTPASLALL LGVTRLAVVP TNNMVTFFKG
     IANGLSENVC LTTDDFQRQE LEGADWWTLL STNQVIDDMG LVLDIHPVWG KSDEEMLMEK
     IQSIGVSNDN NTLSIIVQIL IQAKNAQENL LSQTISAEYG VERSVVPLQL RWLGSNVYSV
     LNQVLNNTPT DISSIVPKLS ELTYSLLIYT QLINSLKLNK EFIFLRLTQP NWLGLTQPKL
     STQLSLPEIY LITCYQDWVV NANKNEDSIH EYLEFANIKK TEAEKTLVDN SEKCAELLAE
     ILAWDAGEIL KAASLLGLNP PQATNVFEID WIRRLQTLSE KTMISTEYLW QMGDLTENSE
     FSLKEGVGEA VMAALKAQGD SDNV