YENA2_YERET
ID YENA2_YERET Reviewed; 1364 AA.
AC B6A878;
DT 05-DEC-2018, integrated into UniProtKB/Swiss-Prot.
DT 25-NOV-2008, sequence version 1.
DT 25-MAY-2022, entry version 31.
DE RecName: Full=Toxin subunit YenA2 {ECO:0000303|PubMed:21278295};
GN Name=yenA2 {ECO:0000303|PubMed:21278295};
OS Yersinia entomophaga.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Yersinia.
OX NCBI_TaxID=935293;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-10 AND 768-786,
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, ACTIVITY REGULATION,
RP SUBUNIT, SUBCELLULAR LOCATION, CLEAVAGE, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC BAA-1678 / DSM 22339 / MH96;
RX PubMed=21278295; DOI=10.1128/jb.01044-10;
RA Hurst M.R., Jones S.A., Binglin T., Harper L.A., Jackson T.A., Glare T.R.;
RT "The main virulence determinant of Yersinia entomophaga MH96 is a broad-
RT host-range toxin complex active against insects.";
RL J. Bacteriol. 193:1966-1980(2011).
RN [2]
RP ELECTRON MICROSCOPY (17.0 ANGSTROMS) OF YEN-TC:K9 COMPLEX, FUNCTION, AND
RP SUBUNIT.
RC STRAIN=ATCC BAA-1678 / DSM 22339 / MH96;
RX PubMed=22158901; DOI=10.1073/pnas.1111155108;
RA Landsberg M.J., Jones S.A., Rothnagel R., Busby J.N., Marshall S.D.,
RA Simpson R.M., Lott J.S., Hankamer B., Hurst M.R.;
RT "3D structure of the Yersinia entomophaga toxin complex and implications
RT for insecticidal activity.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:20544-20549(2011).
CC -!- FUNCTION: Part of an orally active toxin complex (TC) with strong
CC insecticidal effects on larvae of the Coleoptera Costelytra zealandica,
CC Acrossidius tasmania and Adoryphorus couloni and some Lepidoptera
CC larvae (PubMed:21278295, PubMed:22158901). The TC has an endochitinase
CC activity (PubMed:21278295) (Probable). {ECO:0000269|PubMed:21278295,
CC ECO:0000269|PubMed:22158901, ECO:0000305|PubMed:22158901}.
CC -!- SUBUNIT: Semipurified toxin complex consists of at least YenA1-YenA2-
CC YenB-YenC1-YenC2-Chi1-Chi2 (PubMed:21278295). The Yen-TC:K9 subcomplex
CC is about 26 nm tall and 22 nm in diameter with 5-fold symmetry and 5
CC copies of YenA1, YenA2, Chi1 and Chi2; the chitinase subunits may be
CC solvent accessible on the exterior the complex (PubMed:22158901). The
CC Yen-TC:K9 subcomplex has no insecticidal activity (PubMed:22158901).
CC The native complex with additional YenB, YenC1 and YenC2 subunits is 16
CC nm taller and is insecticidal; the toxicity-conferring subunits are
CC present at about 1 copy each (PubMed:22158901).
CC {ECO:0000269|PubMed:21278295, ECO:0000269|PubMed:22158901}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:21278295}.
CC Note=Secreted when grown at 25 degrees Celsius or less, but not when
CC grown at 30 or 37 degrees Celsius. {ECO:0000269|PubMed:21278295}.
CC -!- PTM: The isolated toxin complex includes 3 peptides starting between
CC residues 768 and 778 of this protein, which might be physiologically
CC relevant. {ECO:0000305|PubMed:21278295}.
CC -!- DISRUPTION PHENOTYPE: Cells with a disrupted yenA1-yenA2-chi2-yenB-
CC yenC1-yenC2 locus are no longer pathogenic in C.zealandica larvae.
CC {ECO:0000269|PubMed:21278295}.
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DR EMBL; DQ400808; ABG33868.1; -; Genomic_DNA.
DR RefSeq; WP_064513227.1; NZ_MWTM01000006.1.
DR PDB; 6OGD; EM; 4.40 A; B/E/H/K/N=1-1364.
DR PDBsum; 6OGD; -.
DR AlphaFoldDB; B6A878; -.
DR SMR; B6A878; -.
DR DIP; DIP-60374N; -.
DR IntAct; B6A878; 1.
DR STRING; 935293.PL78_03750; -.
DR PATRIC; fig|935293.3.peg.807; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR InterPro; IPR041079; Neuraminidase-like.
DR InterPro; IPR040840; TcA_TcB_BD.
DR Pfam; PF18413; Neuraminidase; 1.
DR Pfam; PF18276; TcA_TcB_BD; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Coiled coil; Direct protein sequencing; Secreted; Virulence.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:21278295"
FT CHAIN 2..1364
FT /note="Toxin subunit YenA2"
FT /id="PRO_0000445772"
FT COILED 1025..1080
FT /evidence="ECO:0000255"
SQ SEQUENCE 1364 AA; 156177 MW; F7704F923D77DC20 CRC64;
MSNSIEAKLQ EDLRDALVDY YLGQIVPNSK DFTNLRSTIK NVDDLYDHLL LDTQVSAKVI
TSRLSLVTQS VQQYINRIAL NLEPGLSINQ QEATDWEEFA NRYGYWAANQ QLRMFPEIYV
DPTLRLTKTE FFFQLESALN QGKLTDDVAQ KAVLGYLNNF EEVSNLEIIA GYQDGIDIEN
DKTYFVARTR MQPYRYFWRS LDASQRNANS QELYPTAWSE WKAISVPLEN VANGIVRPIM
MDNRLYISWF EVAEEKETDS DGNIIVSGRY RTKIRLAHLG FDGVWSSGTT LREEVLADQM
EEMIAVVDRM EDEPRLALVA FKEMSESWDV VFSYICDSML IESSNLPTTT HPPKPGDGDK
GLSDLDDYGA NLVWFYLHET ANGGKAEYKQ LILYPVIINR DWPIELDKTH QGDFGTVDDF
TLNSNYTGDE LSLYLQSSST YKYDFSKSKN IIYGIWKEDA NNNRCWLNYK LLTPEDYEPQ
INATLVMCDK GDVNIITGFS LPNGGVDAGG KIKVTLRVGK KLRDKFQIKQ FSQTQYLQFP
EASSADVWYI GKQIRLNTLF AKELIGKASR SLDLVLSWET QNSRLEEAIL GGAAELIDLD
GANGIYFWEL FFHMPFMVSW RFNVEQRYED ANRWVKYLFN PFECEDEPAL LLGKPPYWNS
RPLVDEPFKG YSLTQPSDPD AIAASDPIHY RKAVFNFLTK NIIDQGDMEY RKLQPSARTL
ARLSYSTASS LLGRRPDVQL TSFWQPLTLE DASYKTDSEI RAIEMQSQPL TFEPVVHDQT
MSAVDNDIFM YPMNNELRGL WDRIENRIYN LRHNLTLDGK EINMDLYDSS ISPRGLMKQR
YQRVVTARNA SKMNFKVPNY RFEPMLNRSK SGVETLIQFG STLLSLLERK DSLSFDAYQM
IQSGDLYRFS IDLQQQDIDI NKASLEALQV SKQSAQDRYD HFKELYDENI SSTEQKVIEL
QSQAANSLLM AQGMRTAAAA LDVIPNIYGL AVGGSHWGAP LNAAAEIIMI KYQADSSKSE
SLSVSESYRR RRQEWELQYK QAEWEVNSVE QQINLQNMQI KAANKRLEQV EAQQQQAMAL
LDYFSERFTN ESLYTWLISQ LSSLYLQAYD AVLSLCLSAE ASLLYELNLG EQSFVGGGGW
NDLYQGLMAG ETLKLALMRM ERVYVEQNSR RQEITKTISL KALLGESWPA ELNKLKQKTP
INFNLEEQIF VEDYQELYQR RIKSVSVSLP MLVGPYEDVC AQLTQTSSSY STRADLKTVE
NMLTKRTFAD TPHLVRSIQP NQQISLSTGV NDSGLFMLNF DDERFLPFEG SGVDSSWRLQ
FTNLKQNLDS LNDVILHVKY TAAIGSSTFS QGVRKILANI NNDE
