YENC1_YERET
ID YENC1_YERET Reviewed; 974 AA.
AC B6A881;
DT 05-DEC-2018, integrated into UniProtKB/Swiss-Prot.
DT 25-NOV-2008, sequence version 1.
DT 25-MAY-2022, entry version 31.
DE RecName: Full=Toxin subunit YenC1 {ECO:0000303|PubMed:21278295};
GN Name=yenC1 {ECO:0000303|PubMed:21278295};
OS Yersinia entomophaga.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Yersinia.
OX NCBI_TaxID=935293;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-9, IDENTIFICATION
RP BY MASS SPECTROMETRY, FUNCTION, ACTIVITY REGULATION, SUBUNIT, SUBCELLULAR
RP LOCATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC BAA-1678 / DSM 22339 / MH96;
RX PubMed=21278295; DOI=10.1128/jb.01044-10;
RA Hurst M.R., Jones S.A., Binglin T., Harper L.A., Jackson T.A., Glare T.R.;
RT "The main virulence determinant of Yersinia entomophaga MH96 is a broad-
RT host-range toxin complex active against insects.";
RL J. Bacteriol. 193:1966-1980(2011).
RN [2]
RP FUNCTION, AND SUBUNIT.
RC STRAIN=ATCC BAA-1678 / DSM 22339 / MH96;
RX PubMed=22158901; DOI=10.1073/pnas.1111155108;
RA Landsberg M.J., Jones S.A., Rothnagel R., Busby J.N., Marshall S.D.,
RA Simpson R.M., Lott J.S., Hankamer B., Hurst M.R.;
RT "3D structure of the Yersinia entomophaga toxin complex and implications
RT for insecticidal activity.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:20544-20549(2011).
CC -!- FUNCTION: Part of an orally active toxin complex (TC) with strong
CC insecticidal effects on larvae of the Coleoptera Costelytra zealandica,
CC Acrossidius tasmania and Adoryphorus couloni and some Lepidoptera
CC larvae (PubMed:21278295). The TC has an endochitinase activity
CC (PubMed:21278295) (Probable). {ECO:0000269|PubMed:21278295,
CC ECO:0000305|PubMed:22158901}.
CC -!- ACTIVITY REGULATION: Toxin complex is secreted when grown at 25 degrees
CC Celsius or less; at higher temperatures the proteins are present
CC intracellularly but not secreted. {ECO:0000269|PubMed:21278295}.
CC -!- SUBUNIT: Semipurified toxin complex consists of at least YenA1-YenA2-
CC YenB-YenC1-YenC2-Chi1-Chi2 (PubMed:21278295). The Yen-TC:K9 subcomplex
CC is about 26 nm tall and 22 nm in diameter with 5-fold symmetry and 5
CC copies of YenA1, YenA2, Chi1 and Chi2; the chitinase subunits may be
CC solvent accessible on the exterior the complex (PubMed:22158901). The
CC Yen-TC:K9 subcomplex has no insecticidal activity (PubMed:22158901).
CC The native complex with additional YenB, YenC1 and YenC2 subunits is 16
CC nm taller and is insecticidal; the toxicity-conferring subunits are
CC present at about 1 copy each (PubMed:22158901).
CC {ECO:0000269|PubMed:21278295, ECO:0000269|PubMed:22158901}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:21278295}.
CC Note=Secreted when grown at 25 degrees Celsius or less, but not when
CC grown at 30 or 37 degrees Celsius. {ECO:0000269|PubMed:21278295}.
CC -!- DISRUPTION PHENOTYPE: A double yenC1-yenC2 deletion is no longer
CC pathogenic in C.zealandica larvae. {ECO:0000269|PubMed:21278295}.
CC -!- SIMILARITY: Belongs to the RHS family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DQ400808; ABG33865.1; -; Genomic_DNA.
DR RefSeq; WP_064513233.1; NZ_MWTM01000006.1.
DR AlphaFoldDB; B6A881; -.
DR SMR; B6A881; -.
DR PATRIC; fig|935293.3.peg.810; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR CDD; cd16834; CNF1-like; 1.
DR Gene3D; 3.60.100.10; -; 1.
DR InterPro; IPR008430; CNF_Rho-act.
DR InterPro; IPR037040; CNF_Rho-act_sf.
DR InterPro; IPR011324; Cytotoxic_necrot_fac-like_cat.
DR InterPro; IPR022385; Rhs_assc_core.
DR InterPro; IPR041508; TcC-like_repeat.
DR Pfam; PF05785; CNF1; 1.
DR Pfam; PF18807; TTc_toxin_rep; 1.
DR SUPFAM; SSF64438; SSF64438; 1.
DR TIGRFAMs; TIGR03696; Rhs_assc_core; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Repeat; Secreted; Virulence.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:21278295"
FT CHAIN 2..974
FT /note="Toxin subunit YenC1"
FT /id="PRO_0000445774"
FT REPEAT 165..179
FT /note="RHS 1"
FT /evidence="ECO:0000250|UniProtKB:B6A882"
FT REPEAT 290..304
FT /note="RHS 2"
FT /evidence="ECO:0000250|UniProtKB:B6A882"
FT REPEAT 322..336
FT /note="RHS 3"
FT /evidence="ECO:0000250|UniProtKB:B6A882"
FT REPEAT 354..368
FT /note="RHS 4"
FT /evidence="ECO:0000250|UniProtKB:B6A882"
FT REPEAT 398..412
FT /note="RHS 5"
FT /evidence="ECO:0000250|UniProtKB:B6A882"
FT REPEAT 490..504
FT /note="RHS 6"
FT /evidence="ECO:0000250|UniProtKB:B6A882"
FT REPEAT 570..584
FT /note="RHS 7"
FT /evidence="ECO:0000250|UniProtKB:B6A882"
FT REPEAT 596..610
FT /note="RHS 8"
FT /evidence="ECO:0000250|UniProtKB:B6A882"
FT REPEAT 630..644
FT /note="RHS 9"
FT /evidence="ECO:0000250|UniProtKB:B6A882"
FT REGION 600..680
FT /note="RHS-repeat associated core domain"
FT /evidence="ECO:0000305"
FT REGION 699..940
FT /note="Cytotoxic necrotising factor domain"
FT /evidence="ECO:0000305"
FT CONFLICT 5
FT /note="D -> L (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000269|PubMed:21278295"
FT CONFLICT 9
FT /note="H -> T (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000269|PubMed:21278295"
SQ SEQUENCE 974 AA; 108756 MW; 932136906BAB959C CRC64;
MNQFDSALHQ GTPGVSVLDN RGHVIRELRY YRHPDTPQEI AERIAFHQYD SYGFISQSID
PRLAERRKQD SSVKPNLSYF TALSGEVLRT DGVDAGTIFS LNDIAARPAI SISATGVSHT
WQYEGENRPG RVLSRSEREK DREERIIERF YWAGSDASQK ANNLAGQCLR HYNSAGLNQT
LSIALTGTPI SACFQPLLES AEPEWQGTNE SAWLELLTPE IFTTYNRADA NGETLVQTDA
MGNIQRLAYD VAGFLKSSWL SLKGGQEQII VKSLTYSAAG QKLQEEHGNG VLTTYSYEAE
TQRLIGIRTE RPAGHLSGAR VFQDLRYTYD PVGNVLRITN DAEATRFWRN QKVVPENTYI
YDTLYQLVSA NGREMANIPQ QSSQLPTLSP IDNNAYTNYI RNYHYDSAGN LMQIRHTSAA
ANNSYTTNIT VSKYSNRAVL SSLTDDVDKV EAFFDAAGRQ NQLLPGQTLS WNARGELAKV
TPVARDGQES DSETYRYDAN SQRVSKMAIQ QSNNNTQTRR VLYLAGLERR TIHQGNTLFE
TLLVVKIGEA GRAQVQAMHW ELGQPTEVAN DELRYSYDNL IGSSGLEVDG TGQLISQEEY
YPYGGTAVWM ARSQREASDK AYGYSGKERD ATGLYYYGFR YYQPWAGRWL SADPAGTIDG
LNLFRMVRNN PIVLHDPDGL APGFFERISS FRKKDTLTIS SLKGTGPFYT RSESEIDIDF
LFSRQDRDKD FPPQNHKELS AEDRREVLEV SSGENITSAN KSSKWYAGTH WETKPLKNNT
DLVVLHNGVQ GAAGININLN DIKPGRSVLV TAGTLTGCTM ITGVKGNNFY ALHAGTGTPS
ENWVTGEHGV TDNFRMLNKL IPDAGIDLNP EAVNDSLLTI LDYFDNGTIA YNGKKGSEIH
RDADNILNYR TTGYENTVGV SFSLLTKDKN GEVSASTLLE LGELKPHKKH RTRGQFGMTE
LKYEARKNTV VKLR
