YERB_BACSU
ID YERB_BACSU Reviewed; 331 AA.
AC O34968; O30567; Q799E9;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Putative lipoprotein YerB;
DE Flags: Precursor;
GN Name=yerB; Synonyms=yecC; OrderedLocusNames=BSU06570;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969499; DOI=10.1099/13500872-142-11-3027;
RA Borriss R., Porwollik S., Schroeter R.;
RT "The 52 degrees-55 degrees segment of the Bacillus subtilis chromosome: a
RT region devoted to purine uptake and metabolism, and containing the genes
RT cotA, gabP and guaA and the pur gene cluster within a 34960 bp nucleotide
RT sequence.";
RL Microbiology 142:3027-3031(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9701819; DOI=10.1046/j.1365-2958.1998.00927.x;
RA Petit M.-A., Dervyn E., Rose M., Entian K.-D., McGovern S., Ehrlich S.D.,
RA Bruand C.;
RT "PcrA is an essential DNA helicase of Bacillus subtilis fulfilling
RT functions both in repair and rolling-circle replication.";
RL Mol. Microbiol. 29:261-273(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP INTERACTION WITH PCRA, AND DISRUPTION PHENOTYPE.
RX PubMed=12073041; DOI=10.1007/s00438-002-0670-9;
RA Noirot-Gros M.-F., Soultanas P., Wigley D.B., Ehrlich S.D., Noirot P.,
RA Petit M.-A.;
RT "The beta-propeller protein YxaL increases the processivity of the PcrA
RT helicase.";
RL Mol. Genet. Genomics 267:391-400(2002).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-97 AND SER-103, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=168;
RX PubMed=17218307; DOI=10.1074/mcp.m600464-mcp200;
RA Macek B., Mijakovic I., Olsen J.V., Gnad F., Kumar C., Jensen P.R.,
RA Mann M.;
RT "The serine/threonine/tyrosine phosphoproteome of the model bacterium
RT Bacillus subtilis.";
RL Mol. Cell. Proteomics 6:697-707(2007).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 21-331.
RG Northeast structural genomics consortium (NESG);
RT "Crystal structure of yerB protein from Bacillus subtilis.";
RL Submitted (MAY-2007) to the PDB data bank.
CC -!- SUBUNIT: Interacts with PcrA. The interaction is not essential for cell
CC viability or repair of UV-induced lesions.
CC {ECO:0000269|PubMed:12073041}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC ProRule:PRU00303}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC -!- DISRUPTION PHENOTYPE: No PcrA-related phenotype.
CC {ECO:0000269|PubMed:12073041}.
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DR EMBL; AF011544; AAB72189.1; -; Genomic_DNA.
DR EMBL; Y15254; CAA75548.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12477.1; -; Genomic_DNA.
DR PIR; A69794; A69794.
DR RefSeq; NP_388539.1; NC_000964.3.
DR RefSeq; WP_003233927.1; NZ_JNCM01000032.1.
DR PDB; 2PSB; X-ray; 2.10 A; A=21-331.
DR PDBsum; 2PSB; -.
DR AlphaFoldDB; O34968; -.
DR SMR; O34968; -.
DR IntAct; O34968; 1.
DR STRING; 224308.BSU06570; -.
DR iPTMnet; O34968; -.
DR PaxDb; O34968; -.
DR PRIDE; O34968; -.
DR DNASU; 936050; -.
DR EnsemblBacteria; CAB12477; CAB12477; BSU_06570.
DR GeneID; 936050; -.
DR KEGG; bsu:BSU06570; -.
DR PATRIC; fig|224308.179.peg.714; -.
DR eggNOG; COG1470; Bacteria.
DR InParanoid; O34968; -.
DR OMA; PGKTWIN; -.
DR PhylomeDB; O34968; -.
DR BioCyc; BSUB:BSU06570-MON; -.
DR EvolutionaryTrace; O34968; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR Gene3D; 3.50.90.10; -; 1.
DR InterPro; IPR035328; DUF3048_C.
DR InterPro; IPR021416; DUF3048_N.
DR InterPro; IPR023158; YerB-like_sf.
DR Pfam; PF11258; DUF3048; 1.
DR Pfam; PF17479; DUF3048_C; 1.
DR SUPFAM; SSF159774; SSF159774; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Lipoprotein; Membrane; Palmitate;
KW Phosphoprotein; Reference proteome; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 20..331
FT /note="Putative lipoprotein YerB"
FT /id="PRO_0000364085"
FT MOD_RES 97
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:17218307"
FT MOD_RES 103
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:17218307"
FT LIPID 20
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 20
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CONFLICT 2
FT /note="K -> E (in Ref. 1; AAB72189)"
FT /evidence="ECO:0000305"
FT TURN 36..38
FT /evidence="ECO:0007829|PDB:2PSB"
FT HELIX 44..48
FT /evidence="ECO:0007829|PDB:2PSB"
FT STRAND 52..56
FT /evidence="ECO:0007829|PDB:2PSB"
FT HELIX 60..62
FT /evidence="ECO:0007829|PDB:2PSB"
FT STRAND 72..78
FT /evidence="ECO:0007829|PDB:2PSB"
FT STRAND 84..93
FT /evidence="ECO:0007829|PDB:2PSB"
FT STRAND 96..99
FT /evidence="ECO:0007829|PDB:2PSB"
FT HELIX 106..113
FT /evidence="ECO:0007829|PDB:2PSB"
FT TURN 114..116
FT /evidence="ECO:0007829|PDB:2PSB"
FT STRAND 118..122
FT /evidence="ECO:0007829|PDB:2PSB"
FT HELIX 126..133
FT /evidence="ECO:0007829|PDB:2PSB"
FT STRAND 139..141
FT /evidence="ECO:0007829|PDB:2PSB"
FT HELIX 142..145
FT /evidence="ECO:0007829|PDB:2PSB"
FT TURN 147..149
FT /evidence="ECO:0007829|PDB:2PSB"
FT STRAND 150..152
FT /evidence="ECO:0007829|PDB:2PSB"
FT STRAND 154..156
FT /evidence="ECO:0007829|PDB:2PSB"
FT STRAND 162..165
FT /evidence="ECO:0007829|PDB:2PSB"
FT HELIX 166..176
FT /evidence="ECO:0007829|PDB:2PSB"
FT STRAND 202..206
FT /evidence="ECO:0007829|PDB:2PSB"
FT STRAND 215..221
FT /evidence="ECO:0007829|PDB:2PSB"
FT TURN 222..225
FT /evidence="ECO:0007829|PDB:2PSB"
FT STRAND 226..231
FT /evidence="ECO:0007829|PDB:2PSB"
FT TURN 239..241
FT /evidence="ECO:0007829|PDB:2PSB"
FT STRAND 242..244
FT /evidence="ECO:0007829|PDB:2PSB"
FT STRAND 247..254
FT /evidence="ECO:0007829|PDB:2PSB"
FT STRAND 256..259
FT /evidence="ECO:0007829|PDB:2PSB"
FT STRAND 261..263
FT /evidence="ECO:0007829|PDB:2PSB"
FT STRAND 266..269
FT /evidence="ECO:0007829|PDB:2PSB"
FT STRAND 274..280
FT /evidence="ECO:0007829|PDB:2PSB"
FT STRAND 283..292
FT /evidence="ECO:0007829|PDB:2PSB"
FT STRAND 295..300
FT /evidence="ECO:0007829|PDB:2PSB"
FT STRAND 312..318
FT /evidence="ECO:0007829|PDB:2PSB"
FT HELIX 320..322
FT /evidence="ECO:0007829|PDB:2PSB"
FT STRAND 323..326
FT /evidence="ECO:0007829|PDB:2PSB"
SQ SEQUENCE 331 AA; 36969 MW; CD2D795F959E15FC CRC64;
MKKWMTVCAL CFVFFLLVSC QQKDAVPDTA KKLKAPLTGL KTEQKVTERR PVAVVVNNHP
KARPQSGLSK ADIVIEALAE GQITRFLAIF QSQMPETVGP VRSAREYFVT LSNGFDSIFV
HHGWSPGAKK QLESGAADYM NGLDFDGSLF WRADFSKPPH NSYTSYDYIK KAAEQKGYKL
KQETNPLLFQ TSDAKPANES YNVRVDYGTN NVTNLVEYNY DKKAEFYTRS SDGVITTDRE
TGKPVAMQNI FIVEASHHII DQDGRRDIDL ESGGKGLLFQ HGNVIETDWK QVNGRIVPVK
DGKWLPFVPG KTWINIVPDL DAASISKGEG V
