CB60E_ARATH
ID CB60E_ARATH Reviewed; 599 AA.
AC F4IPM3; F4IPM4; Q9ZQ37;
DT 27-MAY-2015, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 66.
DE RecName: Full=Calmodulin-binding protein 60 E {ECO:0000303|PubMed:11782485};
GN Name=CBP60E {ECO:0000303|PubMed:11782485};
GN OrderedLocusNames=At2g24300 {ECO:0000312|Araport:AT2G24300};
GN ORFNames=T28I24.3 {ECO:0000312|EMBL:AAD18103.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11782485; DOI=10.1074/jbc.m111626200;
RA Reddy V.S., Ali G.S., Reddy A.S.N.;
RT "Genes encoding calmodulin-binding proteins in the Arabidopsis genome.";
RL J. Biol. Chem. 277:9840-9852(2002).
CC -!- FUNCTION: Transcription activator that binds DNA in a sequence-specific
CC manner, likely 5'-GAAATTTTGG-3', to promote the expression of target
CC genes. {ECO:0000250|UniProtKB:F4K2R6}.
CC -!- SUBUNIT: Interacts with calmodulin (CaM).
CC {ECO:0000250|UniProtKB:F4K2R6}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9C9T2}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=F4IPM3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4IPM3-2; Sequence=VSP_057664;
CC Name=3;
CC IsoId=F4IPM3-3; Sequence=VSP_057665;
CC -!- SIMILARITY: Belongs to the plant ACBP60 protein family. {ECO:0000305}.
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DR EMBL; AC006403; AAD18103.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07559.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07560.1; -; Genomic_DNA.
DR EMBL; BT011245; AAR92281.1; -; mRNA.
DR EMBL; BT012547; AAS99691.1; -; mRNA.
DR PIR; A84635; A84635.
DR RefSeq; NP_180007.2; NM_127992.3. [F4IPM3-3]
DR RefSeq; NP_973527.1; NM_201798.1. [F4IPM3-1]
DR AlphaFoldDB; F4IPM3; -.
DR PaxDb; F4IPM3; -.
DR PRIDE; F4IPM3; -.
DR ProteomicsDB; 239096; -. [F4IPM3-1]
DR EnsemblPlants; AT2G24300.1; AT2G24300.1; AT2G24300. [F4IPM3-3]
DR EnsemblPlants; AT2G24300.2; AT2G24300.2; AT2G24300. [F4IPM3-1]
DR GeneID; 816966; -.
DR Gramene; AT2G24300.1; AT2G24300.1; AT2G24300. [F4IPM3-3]
DR Gramene; AT2G24300.2; AT2G24300.2; AT2G24300. [F4IPM3-1]
DR KEGG; ath:AT2G24300; -.
DR Araport; AT2G24300; -.
DR TAIR; locus:2061112; AT2G24300.
DR eggNOG; ENOG502R2A5; Eukaryota.
DR HOGENOM; CLU_031504_3_0_1; -.
DR InParanoid; F4IPM3; -.
DR OMA; FEGHEVK; -.
DR PhylomeDB; F4IPM3; -.
DR PRO; PR:F4IPM3; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; F4IPM3; baseline and differential.
DR Genevisible; F4IPM3; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0080142; P:regulation of salicylic acid biosynthetic process; IBA:GO_Central.
DR InterPro; IPR012416; CBP60.
DR PANTHER; PTHR31713; PTHR31713; 1.
DR Pfam; PF07887; Calmodulin_bind; 1.
PE 2: Evidence at transcript level;
KW Activator; Alternative splicing; Calmodulin-binding; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..599
FT /note="Calmodulin-binding protein 60 E"
FT /id="PRO_0000433049"
FT REGION 1..80
FT /note="Calmodulin-binding"
FT /evidence="ECO:0000250|UniProtKB:F4K2R6"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 150..273
FT /note="DNA-binding"
FT /evidence="ECO:0000250|UniProtKB:F4K2R6"
FT VAR_SEQ 1..96
FT /note="Missing (in isoform 2)"
FT /id="VSP_057664"
FT VAR_SEQ 1..54
FT /note="MNKRGYECSQEDTDKLPESKRQKVPALASVIVEAVKVDSLQRLCSSLEPLFR
FT RI -> MVWTNTQ (in isoform 3)"
FT /id="VSP_057665"
SQ SEQUENCE 599 AA; 67969 MW; F2F8755F2B997428 CRC64;
MNKRGYECSQ EDTDKLPESK RQKVPALASV IVEAVKVDSL QRLCSSLEPL FRRIVSEEVE
RALSRLGNAK LTSRSPEPKR IQDRNGRNLQ LHFRTRMPPH LFTGGKVEGE RGSAIHVVLI
DANTGNVVQT GEESASKLNV VVLEGDFNDE DDEDWTREHF ESFEVKEREG KRPILTGDTQ
IVLKEGVGTL GELTFTDNSS WIRSRKFRLG VKPASGYGDS FCIREAKTEP FAVKDHRGEL
YKKHYPPAVH DEVWRLDRIA KDGVLHKKLL KANIVTVEDF LRLLVKDPQK LRNLLGSGMS
NRMWENTVEH AKTCVLGGKL YVFYTDQTHA TGVVFNHIYE FRGLITNGQF LSLESLNHDQ
KISADILVKL AYENWHKAIE YDGKLLNCLP VAEKEIKSLL EPKMVSAQTA PNHQQLHNQN
NRQTVQGHQN AITYSPVPQP IDYPQFAQQH CNQLLPSFPC NVQDYNRSME SSNDSSSYNG
EDWCPPRAAG QGLEDIFSEE IRLRSSEMLE TDDMQRLLKT FGIGVNTVGT QGGFGQTDES
CYGYSIPYQA QIDNTYRRER NRGSGKAVVG WLKLKAALRW GIFIRKKAAE RRPQIVEID
