CB60F_ARATH
ID CB60F_ARATH Reviewed; 562 AA.
AC F4JR57; O65550; Q8W454;
DT 27-MAY-2015, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 63.
DE RecName: Full=Calmodulin-binding protein 60 F {ECO:0000303|PubMed:11782485};
GN Name=CBP60F {ECO:0000303|PubMed:11782485};
GN OrderedLocusNames=At4g31000 {ECO:0000312|Araport:AT4G31000};
GN ORFNames=F6I18.90 {ECO:0000312|EMBL:CAA18193.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11782485; DOI=10.1074/jbc.m111626200;
RA Reddy V.S., Ali G.S., Reddy A.S.N.;
RT "Genes encoding calmodulin-binding proteins in the Arabidopsis genome.";
RL J. Biol. Chem. 277:9840-9852(2002).
CC -!- FUNCTION: Transcription activator that binds DNA in a sequence-specific
CC manner, likely 5'-GAAATTTTGG-3', to promote the expression of target
CC genes. {ECO:0000250|UniProtKB:F4K2R6}.
CC -!- SUBUNIT: Interacts with calmodulin (CaM).
CC {ECO:0000250|UniProtKB:F4K2R6}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9C9T2}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=Additional isoforms seem to exist. {ECO:0000305};
CC Name=1;
CC IsoId=F4JR57-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4JR57-2; Sequence=VSP_057666;
CC -!- SIMILARITY: Belongs to the plant ACBP60 protein family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA18193.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB79818.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL022198; CAA18193.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161578; CAB79818.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE85843.1; -; Genomic_DNA.
DR EMBL; AY062856; AAL32934.1; -; mRNA.
DR EMBL; AY114575; AAM47894.1; -; mRNA.
DR PIR; A85363; A85363.
DR RefSeq; NP_194829.2; NM_119250.3. [F4JR57-1]
DR AlphaFoldDB; F4JR57; -.
DR PaxDb; F4JR57; -.
DR PRIDE; F4JR57; -.
DR EnsemblPlants; AT4G31000.1; AT4G31000.1; AT4G31000. [F4JR57-1]
DR GeneID; 829227; -.
DR Gramene; AT4G31000.1; AT4G31000.1; AT4G31000. [F4JR57-1]
DR KEGG; ath:AT4G31000; -.
DR Araport; AT4G31000; -.
DR TAIR; locus:2126679; AT4G31000.
DR eggNOG; ENOG502R2A5; Eukaryota.
DR HOGENOM; CLU_031504_3_0_1; -.
DR InParanoid; F4JR57; -.
DR OMA; HHIREAK; -.
DR OrthoDB; 347274at2759; -.
DR PRO; PR:F4JR57; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; F4JR57; baseline and differential.
DR Genevisible; F4JR57; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0080142; P:regulation of salicylic acid biosynthetic process; IBA:GO_Central.
DR InterPro; IPR012416; CBP60.
DR PANTHER; PTHR31713; PTHR31713; 1.
DR Pfam; PF07887; Calmodulin_bind; 1.
PE 2: Evidence at transcript level;
KW Activator; Alternative splicing; Calmodulin-binding; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..562
FT /note="Calmodulin-binding protein 60 F"
FT /id="PRO_0000433050"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 5..84
FT /note="Calmodulin-binding"
FT /evidence="ECO:0000250|UniProtKB:F4K2R6"
FT REGION 154..273
FT /note="DNA-binding"
FT /evidence="ECO:0000250|UniProtKB:F4K2R6"
FT VAR_SEQ 1..284
FT /note="Missing (in isoform 2)"
FT /id="VSP_057666"
FT CONFLICT 240
FT /note="L -> V (in Ref. 1; CAA18193/CAB79818)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 562 AA; 63685 MW; 763168ECE68FE37D CRC64;
MENSMNNRGH GHNQEHADNL PESKRQKLPA LASVIVEAVK VDSLQRLCSS LEPLFRRIVS
EEVERAISRL ENSKSTSRST EPNKIQGLDG RNLQLRFRTR MPPHLFTGGK VEGEQGSAIH
VVLIDANTGN VIQTGEESMT KLNIVVLDGD FNDEDDKDWT REHFESFEVK EREGKRPILT
GDRHVIIKEG VGTLGKLTFT DNSSWIRSRK FRLGVKPATG FHIREAKTEP FAVKDHRGEL
YKKHYPPVLH DEVWRLDKIA KDGALHKKLL KSNIVTVEDF LQILMKDPQK LRSLLGSGMS
NRMWDNTVEH AKTCVLGGKL YAYYTDQTHQ TAVVFNHIYE FQGLIANGHF LSSESLNHDQ
KISADTLVKT AYENWHKVVE YGGKLLNCLP VAKKGIKSLP EPKMAQNHQT QQKALQCQQT
VNGYSSDPQH LIEYPFVQQP CYQLRDYTSM ESSSVSGSYN GGLEDIFTEE IRARSSEMLE
TDNMQRLLKT FGISGGFGNR DESIYGFSDQ YEAQIDKGYM REGGRGAGKA VVGWLKLKAA
LRWGIFIRKK AAERRPQIVE ID
