ID   CBAA_COMTE              Reviewed;         432 AA.
AC   Q44256; O08105;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=3-chlorobenzoate-3,4-dioxygenase oxygenase subunit;
DE            EC=1.14.-.-;
GN   Name=cbaA;
OS   Comamonas testosteroni (Pseudomonas testosteroni).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Comamonas.
OX   NCBI_TaxID=285;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BR60 / Isolate Bloody Run creek; TRANSPOSON=Tn5271;
RX   PubMed=7704279; DOI=10.1099/13500872-141-2-485;
RA   Nakatsu C.H., Straus N.A., Wyndham R.C.;
RT   "The nucleotide sequence of the Tn5271 3-chlorobenzoate 3,4-dioxygenase
RT   genes (cbaAB) unites the class IA oxygenases in a single lineage.";
RL   Microbiology 141:485-495(1995).
CC   -!- COFACTOR:
CC       Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135; Evidence={ECO:0000305};
CC       Note=Binds 1 [2Fe-2S] cluster. {ECO:0000305};
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000305};
CC       Note=Binds 1 Fe cation. {ECO:0000305};
CC   -!- SUBUNIT: This dioxygenase system consists of two proteins: an oxygenase
CC       and an oxygenase reductase.
CC   -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating dioxygenase
CC       alpha subunit family. {ECO:0000305}.
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DR   EMBL; U18133; AAC45716.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q44256; -.
DR   SMR; Q44256; -.
DR   BioCyc; MetaCyc:MON-14756; -.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0051213; F:dioxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 2.102.10.10; -; 1.
DR   InterPro; IPR045623; LigXa_C.
DR   InterPro; IPR017941; Rieske_2Fe-2S.
DR   InterPro; IPR036922; Rieske_2Fe-2S_sf.
DR   InterPro; IPR015881; Ring-hydroxy_dOase_2Fe2S_BS.
DR   Pfam; PF19301; LigXa_C; 1.
DR   Pfam; PF00355; Rieske; 1.
DR   SUPFAM; SSF50022; SSF50022; 1.
DR   PROSITE; PS51296; RIESKE; 1.
DR   PROSITE; PS00570; RING_HYDROXYL_ALPHA; 1.
PE   3: Inferred from homology;
KW   2Fe-2S; Aromatic hydrocarbons catabolism; Dioxygenase; Iron; Iron-sulfur;
KW   Metal-binding; NAD; Oxidoreductase.
FT   CHAIN           1..432
FT                   /note="3-chlorobenzoate-3,4-dioxygenase oxygenase subunit"
FT                   /id="PRO_0000085050"
FT   DOMAIN          27..133
FT                   /note="Rieske"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT   BINDING         69
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT   BINDING         71
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT   BINDING         88
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT   BINDING         91
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT   BINDING         180
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   BINDING         185
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   432 AA;  48927 MW;  216CF50FE14EBEE2 CRC64;
     MALTKDNEDL VRVGRGTPMG GLMREYWIPA LKSTELEAGG SPVRLLLLGE KLVAFREPSG
     AVGVMDSRCP HRGVSLFMGR VEEGGLRCVY HGWKFSAEGK CVDMPSVRPE DEFKNSVRVA
     RYPVKEMAGV VWVYMGTRKV LPELPRLEVL NLPENEVDVI CLQRKSNWLQ NLEGEIDTSH
     FNFLHVGGLH ADEVPDDHPL KYTAQVAPQY LVKETALGTC YAAQVPAEED HTYTRFAHFL
     FPFWALIPQA DIAQNILARA WVPMDDEHTM MFFFRWTGSK AKRLDTPLKS GSPMPGVTLT
     DMKYKENTTD WYGRWQPLGD ESNDWLIDRD LQKVGRVFSG IYGIHAQDQA MTDSMGPIID
     HGLEQLAPTD LMIVRTRRRI LKALRAHEAN GTLPPGVDEA DQYFTPRSGY YLTPKSVDWE
     TAYEQRIEGL VR