ID   YEZG_BACSH              Reviewed;         161 AA.
AC   E0TU95;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2010, sequence version 1.
DT   03-AUG-2022, entry version 47.
DE   RecName: Full=Immunity protein YezG {ECO:0000303|PubMed:32117125};
GN   Name=yezG {ECO:0000303|PubMed:32117125};
GN   OrderedLocusNames=BSUW23_03450 {ECO:0000312|EMBL:ADM36746.1};
OS   Bacillus spizizenii (strain ATCC 23059 / NRRL B-14472 / W23) (Bacillus
OS   subtilis subsp. spizizenii).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=655816;
RN   [1] {ECO:0000312|EMBL:ADM36746.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 23059 / NRRL B-14472 / W23;
RX   PubMed=21527469; DOI=10.1099/mic.0.048520-0;
RA   Zeigler D.R.;
RT   "The genome sequence of Bacillus subtilis subsp. spizizenii W23: insights
RT   into speciation within the B. subtilis complex and into the history of B.
RT   subtilis genetics.";
RL   Microbiology 157:2033-2041(2011).
RN   [2]
RP   FUNCTION AS AN IMMUNITY PROTEIN, SUBUNIT, SUBCELLULAR LOCATION, AND
RP   EXPRESSION IN E.COLI.
RX   PubMed=32117125; DOI=10.3389/fmicb.2020.00095;
RA   Kaundal S., Deep A., Kaur G., Thakur K.G.;
RT   "Molecular and Biochemical Characterization of YeeF/YezG, a Polymorphic
RT   Toxin-Immunity Protein Pair From Bacillus subtilis.";
RL   Front. Microbiol. 11:95-95(2020).
CC   -!- FUNCTION: Immunity component of an LXG toxin-immunity module. These
CC       modules promote kin selection, mediate competition in biofilms, and
CC       drive spatial segregation of different strains, indicating that LXG
CC       toxins may help avoid warfare between strains in biofilms. Neutralizes
CC       the toxic abilities of cognate toxin YeeF upon expression in E.coli and
CC       in vitro. {ECO:0000269|PubMed:32117125}.
CC   -!- SUBUNIT: Monomer (PubMed:32117125). Interacts with the C-terminus of
CC       cognate toxin YeeF, probably with 2:2 stoichiometry. The second YezG
CC       molecules binds with lower affinity (Probable).
CC       {ECO:0000269|PubMed:32117125, ECO:0000305|PubMed:32117125}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|PubMed:32117125}.
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DR   EMBL; CP002183; ADM36746.1; -; Genomic_DNA.
DR   SASBDB; E0TU95; -.
DR   EnsemblBacteria; ADM36746; ADM36746; BSUW23_03450.
DR   KEGG; bss:BSUW23_03450; -.
DR   HOGENOM; CLU_107164_0_0_9; -.
DR   OMA; DIFMDLW; -.
DR   Proteomes; UP000002233; Chromosome.
DR   InterPro; IPR006728; EsaG-like.
DR   InterPro; IPR036170; Prob_YezG_sf.
DR   Pfam; PF04634; DUF600; 1.
DR   SUPFAM; SSF160424; SSF160424; 1.
DR   TIGRFAMs; TIGR01741; staph_tand_hypo; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm.
FT   CHAIN           1..161
FT                   /note="Immunity protein YezG"
FT                   /id="PRO_0000456173"
SQ   SEQUENCE   161 AA;  19234 MW;  93CE1489414100F1 CRC64;
     MDTPKMGDLY QRIANQINEM IPSEWENVYL YAEILDDSSE VYFYFNIPGK NEFLYSHNIP
     EHFNVSEDIY DDLLIELQES FEELREEYEK NNPETWTNLT LKLDRTGQFS IDYNYEDVIA
     SELNGSQRKA VWVYKNLGLM PKRKTVRDFL EDYIKTNEGK I