ID   YEZG_BACSU              Reviewed;         151 AA.
AC   C0H3X4; O30580;
DT   24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=Immunity protein YezG;
GN   Name=yezG; Synonyms=yeeE; OrderedLocusNames=BSU06811;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Borriss R., Schroeter R.;
RT   "The 55-58 degree segment of the Bacillus subtilis chromosome, a region
RT   spanning from the purA gene cluster to the cotJ operon.";
RL   Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   INDENTIFICATION OF TOXIN-IMMUNITY PAIR, AND INTERACTION WITH YEEF.
RC   STRAIN=168;
RX   PubMed=22200572; DOI=10.1016/j.febslet.2011.12.020;
RA   Holberger L.E., Garza-Sanchez F., Lamoureux J., Low D.A., Hayes C.S.;
RT   "A novel family of toxin/antitoxin proteins in Bacillus species.";
RL   FEBS Lett. 586:132-136(2012).
RN   [4]
RP   FUNCTION AS AN IMMUNITY PROTEIN, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / NRRL NRS-744 / VKM B-501;
RX   PubMed=34280190; DOI=10.1371/journal.pgen.1009682;
RA   Kobayashi K.;
RT   "Diverse LXG toxin and antitoxin systems specifically mediate intraspecies
RT   competition in Bacillus subtilis biofilms.";
RL   PLoS Genet. 17:e1009682-e1009682(2021).
CC   -!- FUNCTION: Immunity component of one of 6 LXG toxin-immunity modules in
CC       this strain. They promote kin selection, mediate competition in
CC       biofilms, and drive spatial segregation of different strains,
CC       indicating that LXG toxins may help avoid warfare between strains in
CC       biofilms. Mediates intercellular competition during biofilm formation;
CC       disruption of the operon disadvantages the bacteria, but overexpression
CC       of the cognate immunity protein restores growth in competition with
CC       wild-type. In situ neutralizes the toxic effect of cognate toxin YeeF
CC       (PubMed:34280190). Probably neutralizes the ability to inhibit growth
CC       of cognate toxin YeeF. Probably does not have immunity protein activity
CC       on other LXG toxins (Probable). {ECO:0000269|PubMed:34280190,
CC       ECO:0000305|PubMed:22200572}.
CC   -!- SUBUNIT: Interacts with cognate toxin YeeF but not with non-cognate
CC       toxin YobL. The interaction probably inhibits the toxic activity of
CC       YeeF (PubMed:22200572). May bind with a stoichiometry of 2:2 to YeeF
CC       (By similarity). {ECO:0000250|UniProtKB:E0TU95,
CC       ECO:0000269|PubMed:22200572}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- INDUCTION: Expressed on rich and minimal solid media likely in early
CC       stationary phase; dependent on DegSU. Not expressed in liquid LB, but
CC       only under conditions that promote biofilm formation.
CC       {ECO:0000269|PubMed:34280190}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the yeeF-yezG operon has no visible
CC       growth phenotype, however it is out-competed by wild-type cells.
CC       {ECO:0000269|PubMed:34280190}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB66478.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AF012532; AAB66478.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AL009126; CAX52579.1; -; Genomic_DNA.
DR   RefSeq; WP_010886434.1; NZ_JNCM01000032.1.
DR   RefSeq; YP_003097692.1; NC_000964.3.
DR   AlphaFoldDB; C0H3X4; -.
DR   SMR; C0H3X4; -.
DR   IntAct; C0H3X4; 1.
DR   MINT; C0H3X4; -.
DR   STRING; 224308.BSU06811; -.
DR   PRIDE; C0H3X4; -.
DR   EnsemblBacteria; CAX52579; CAX52579; BSU_06811.
DR   GeneID; 8303066; -.
DR   KEGG; bsu:BSU06811; -.
DR   PATRIC; fig|224308.43.peg.718; -.
DR   eggNOG; ENOG5032YNG; Bacteria.
DR   OMA; YMNISRD; -.
DR   PhylomeDB; C0H3X4; -.
DR   BioCyc; BSUB:BSU06811-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   InterPro; IPR006728; EsaG-like.
DR   InterPro; IPR036170; Prob_YezG_sf.
DR   Pfam; PF04634; DUF600; 1.
DR   SUPFAM; SSF160424; SSF160424; 1.
DR   TIGRFAMs; TIGR01741; staph_tand_hypo; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Cytoplasm; Reference proteome.
FT   CHAIN           1..151
FT                   /note="Immunity protein YezG"
FT                   /id="PRO_0000389483"
FT   COILED          62..90
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   151 AA;  18022 MW;  66324815F2ED89B7 CRC64;
     MQDLYQLIGE KLNDIIPGEW TKIYLYAEVL DDSTMVLFHF RTPENNQIIY SQDIPSHYNV
     SKDIFKTLLR ELRELFEELR TEHRNNNDDV WTNLTLTLDR SGEFQLDYNY DDILASELDG
     YERIAIWEYK NLGILPEDED DKEFVISYLG L